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Minute™ Synaptosome Isolation Kit (50 preps)

Cat #: SY-052

Manual & Protocol | MSDS

The ability to isolate synaptosomes from neuronal tissues/cultured cells is essential for understanding the mechanisms of neurological diseases. Contained in synaptosomes are synaptic vesicles with a diameter of 80-200 nm that play an important role in signal transmission. Synaptosomes are traditionally isolated by density ultracentrifugation, which is relatively tedious and time-consuming and usually requires a larger amount of starting material. MinuteTM synaptosome isolation kit provides a simple and rapid method for isolating synaptosomes from fresh/frozen neuronal tissues/cultured cells. The protocol can be completed in less than 1 hour without using a Dounce homogenizer and ultracentrifugation. The amount of starting material required (10-50 mg tissue) is only a fraction of that required by the traditional methods. All buffers in the kit are detergent-free.  The synaptosome-associated proteins are isolated in native form.

 

Fig.1. Isolation of Synaptosomes from Fresh Mouse Cortex.

A, Ponceau S-stained blot. B, Western blot. Lane 1. Total tissue lysate;

Lane 2. Cytosolic fraction; Lane 3. Synaptosome fraction. Synaptosome

marker antibodies (GluN2B and Glu A2/A3/A4) are from Cell Signaling.

 

Kit Component:

Buffer A 25 ml
Buffer B 3 ml
Buffer C 25 ml
Plastic Rods 2 units
Filter Cartridges 50 units
2 ml Collection Tubes 50 units

 

 References (4)

  1.  Gu, J., Ke, P., Guo, H., Liu, J., Liu, Y., Tian, X., ... & Xiao, F. (2023). KCTD13-mediated ubiquitination and degradation of GluN1 regulates excitatory synaptic transmission and seizure susceptibility. Cell Death & Differentiation, 1-16.

  2. Ke, P., Gu, J., Liu, J., Liu, Y., Tian, X., Ma, Y., ... & Xiao, F. (2023). Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B. Cell Death Discovery, 9(1), 187.
  3. Han, X., Huang, S., Zhuang, Z., Zhang, X., Xie, M., Lou, N., ... & Chen, S. (2023). Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics. Redox Biology, 102996.

  4. Zong R, Zhang X, Dong X, Liu G, Zhang J,Gao Y, Zhang Z, Ma Y, Gao H and Gamper N (2024) Genetic deletion of zinc transporter ZnT3 induces progressive cognitive deficits in mice by impairing dendritic spine plasticity and glucose metabolism. Front. Mol. Neurosci. 17:1375925. doi: 10.3389/fnmol.2024.1375925