Minute™ Synaptosome Isolation Kit (50 preps) – Invent Biotechnologies Inc.

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Minute™ Synaptosome Isolation Kit (50 preps)

Cat # SY-052

  • $298.00



Manual & Protocol | Material Safety Data Sheets (MSDS)

The ability to isolate synaptosomes from neuronal tissues/cultured cells is essential for understanding the mechanisms of neurological diseases. Contained in synaptosomes are synaptic vesicles with diameters of 80-200 nm that play an important role in signal transmission. Traditionally, synaptosomes are isolated by density ultracentrifugation. The procedure is relatively tedious and time-consuming. A larger amount of starting material is usually required. MinuteTM synaptosome isolation kit provides a simple and rapid method for isolating synaptosomes from fresh/frozen neuronal tissues/cultured cells. The protocol can be completed in less than 1 h without using Dounce homogenizer and ultracentrifugation. The amount of starting material required (10-50 mg tissue) is only a fraction of that required by the traditional method. The buffers used are detergent-free and synaptosome associated proteins are isolated in native form.

 

Fig.1. Isolation of Synaptosomes from Fresh Mouse Cortex.

A, Ponceau S-stained blot. B, Western blot. Lane 1. Total tissue lysate;

Lane 2. Cytosolic fraction; Lane 3. Synaptosome fraction. Synaptosome

marker antibodies (GluN2B and Glu A2/A3/A4) are from Cell Signaling.

 

Kit Component:

Buffer A 25 ml
Buffer B 3 ml
Buffer C 25 ml
Plastic Rods 2
Filter Cartridges 50
2 ml Collection Tubes 50

 

 



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