Comparison of Major Commercial Plant Nuclei Isolation Kits
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The nuclear proteome has recently gained importance in basic and applied research due to the fact that identification of novel nuclear proteins helps to understand protein functions and protein-protein/protein-nucleic acid interactions. Currently there just a few commercial kits available for isolation of plant nuclei. Following table is a side by side comparison of two major brands.
| Brand/Manufacturer | Leading Brand | MinuteTM (Invent Biotechnologies, Inc.) |
|---|---|---|
| Product Name | Plant Nuclei Isolation /Extraction Kit | MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit |
| Mechanism of Isolation | Solution-based | Spin column-based |
| Average sample size | 20 g | 150 mg |
| List of required buffers | Nuclei Isolation Buffer 4X (NIB) Percoll Sucrose 2.3 M TRITONX-100 10% Extraction Buffer Nuclei PURE Storage Buffer |
Buffer A Buffer B Buffer C |
| Liquid nitrogen | Required | Not required |
| Buffer dilution prior to experiment | Required | Not required |
| Fractions obtained | Nuclei | Cytosol Microsomal Chloroplast Nuclei |
| Purity of isolated Nuclei | Low to high | High |
| Protocol Time | >90 min | <60 min |