Comparison of Major Commercial Plant Nuclei Isolation Kits – Invent Biotechnologies Inc.

Comparison of Major Commercial Plant Nuclei Isolation Kits

Posted by Jorge Mogollon on

The nuclear proteome has recently gained importance in basic and applied research due to the fact that identification of novel nuclear proteins helps to understand protein functions and protein-protein/protein-nucleic acid interactions. Currently there just a few commercial kits available for isolation of plant nuclei. Following table is a side by side comparison of two major brands. 

Brand/Manufacturer Leading Brand MinuteTM
(Invent Biotechnologies, Inc.)
Product Name Plant Nuclei Isolation /Extraction Kit MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit
Mechanism of Isolation Solution-based Spin column-based
Average sample size 20 g 150 mg
List of required buffers Nuclei Isolation Buffer 4X (NIB)
Percoll
Sucrose 2.3 M
TRITONX-100 10%
Extraction Buffer
Nuclei PURE Storage Buffer
Buffer A
Buffer B
Buffer C
Liquid nitrogen Required Not required
Buffer dilution prior to experiment Required Not required
Fractions obtained Nuclei Cytosol
Microsomal
Chloroplast
Nuclei
Purity of isolated Nuclei Low to high High
Protocol Time >90 min <60 min

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