Comparison of Major Commercial Plant Nuclei Isolation Kits
Posted by Jorge Mogollon on
The nuclear proteome has recently gained importance in basic and applied research due to the fact that identification of novel nuclear proteins helps to understand protein functions and protein-protein/protein-nucleic acid interactions. Currently there just a few commercial kits available for isolation of plant nuclei. Following table is a side by side comparison of two major brands.
| Brand/Manufacturer | Leading Brand | MinuteTM (Invent Biotechnologies, Inc.) |
|---|---|---|
| Product Name | Plant Nuclei Isolation /Extraction Kit | MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit |
| Mechanism of Isolation | Solution-based | Spin column-based |
| Average sample size | 20 g | 150 mg |
| List of required buffers | Nuclei Isolation Buffer 4X (NIB) Percoll Sucrose 2.3 M TRITONX-100 10% Extraction Buffer Nuclei PURE Storage Buffer |
Buffer A Buffer B Buffer C |
| Liquid nitrogen | Required | Not required |
| Buffer dilution prior to experiment | Required | Not required |
| Fractions obtained | Nuclei | Cytosol Microsomal Chloroplast Nuclei |
| Purity of isolated Nuclei | Low to high | High |
| Protocol Time | >90 min | <60 min |