Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

*Invent Biotechnologies, Inc. is the inventor of the spin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the technology. 

Adipocytes are the major energy storage sites in the body, and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into a single nucleus and cytosolic fraction is a common lab practice. However, when it comes to adipose tissue and adipocytes, separating these two fractions is quite challenging due to the low protein content and the presence of abundant lipid droplets. Methods developed by different labs are tedious and unpractical, requiring up to 50 grams of starting materials. This kit provides a quick and simple protocol for obtaining high-quality single nuclei and cytosol fractions from adipose tissue and adipocytes. Only milligrams of starting material are required, making it possible to isolate nuclei and extract proteins from biopsies and limited tissues. The intact nuclei isolated can be used in many applications such as scRNA-seq, ATAC-seq, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, and other applications.

For storage and transportation of isolated single nuclei, the following buffer is recommended:

WA-014: Minute™ Anti-Clumping Nuclei Storage Buffer 

NOTE: We also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:

NI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit

BN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

SN-047: Minute™ Single Nucleus Isolation Kit for Tissues/Cells

Kit includes:

1. Rajbhandari, P., Arneson, D., Feng, A. C., Ahn, I. S., Diamante, G., Zaghari, N., ... & Smale, S. T. (2019). Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes. eLife 2019;8:e49501.
2.    Felemban, A. H., Alshammari, G. M., Yagoub, A. E. A., Saleh, A., & Yahya, M. A. (2024). Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing Adipogenesis. Nutrients, 16(18), 3174.