Cat# YN-058
Manual & Protocol | Material Safety Data Sheets (MSDS)
The Minute™ Yeast/Fungus Nuclear and Cytoplasmic Protein Extraction Kit offers a fast and reliable method to isolate both cytosolic and nuclear proteins from yeast and fungus. This easy-to-use kit allows you to separate these crucial cellular components in about 1h, streamlining your research workflow. The kit provides all the necessary buffers and disposable tools for an efficient protein extraction. Using this kit, you'll have access to both cytosolic and intact nuclei that can be used for nuclear protein and nucleic acid extraction.
Fig. 1. Isolation of cytosolic and nuclear fractions from S. cerevisiae.
A. Ponceau red stained protein profiles on nitrocellulose membrane.
B. Western blotting using anti-GAPDH and Histone3.
Lane 1, total cell lysate. Lane 2, Nuclear fraction. Lane 3, cytosolic fraction.
Fig. 2. Enhancement of Nuclear Signal by Western Blotting. Equal amounts of total cell lysate of S. cerevisiae (lane 1 and 2) and nuclear protein extract (lane 3 and 4) were separated on SDS-PAGE and probed with anti-histone3 antibody. Data provided by a third-party testing.
Fig. 3. Nuclei Isolated from S. cerevisiae. DAPI staining (Mag. 20 X)
Fig. 4. Nuclei Isolated from A. fumigatus Stained with DAPI. (Mag. 20 X).
(Courtesy of Sandeep Dartmouth School of Medicine.)
Kit includes:
Items
Quantity
Buffer A
20 ml
Buffer B
Buffer C
2 ml
1.5 ml Microfuge Tubes
20 units
Pestles for 1.5 ml Tubes
2 units
Protein Extraction Powder
5 grams
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