Minute™ Plant Lipid Raft Isolation Kit (20 preps)

 Manual & Protocol | MSDS

There is substantial evidence that supports the existence of detergent-resistant membrane (DRM) subdomains in both animal and plant membrane systems, which are also referred to as lipid rafts. Lipid rafts are shown to have an increased sphingolipid-to-protein ratio and higher cholesterol concentration. Lipid rafts are believed to play an important role in signal transduction and protein trafficking in eukaryotic cells. The traditional method for lipid raft isolation involves cold non-ionic detergent extraction followed by sucrose gradient ultracentrifugation. The protocol requires a large amount of starting material (hundreds of grams) and specialized equipment in addition to a lengthy protocol time. We have developed a rapid method for isolation of lipid rafts from plant tissues using only milligram amount of starting material, and the protocol can be completed in about one hour without using ultracentrifugation.

Western Blotting of Isolated Lipid Rafts. A. Ponceau red stained blot membrane. B. Western Blot. Samples: Leaves of Spinacia oleracea (1,2,3) and Blassica rapa (Lanes 4,5,6). Lanes 1 & 4, total tissue lysates. Lanes 2,3 & 4,5; isolated Lipid rafts.  Rabbit anti H+ATPase (plasma membrane marker), and cFBPase (cytosolic marker) were purchased from Agrisera (Vannas, Sweden).

Kit includes:

Reference (1)

1. Zhou, N., Li, X., Zheng, Z., Liu, J., Downie, J. A., & Xie, F. (2024). RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip. Nature Communications, 15(1), 1-16.