Minute™ Plant ER Enrichment Kit (20 preps)

 Manual & Protocol | MSDS

Isolation and enrichment of the plant endoplasmic reticulum (ER) are essential for elucidating ER-related cellular functions. In animal cells, the ER is closely associated with microtubules, while the Golgi apparatus clusters at the microtubule-organizing center. In plant cells, the ER is associated with actin microfilaments, and no microtubule-organizing center is found near the nucleus. These structural differences make plant ER much more difficult and time-consuming to isolate and enrich than ER from animal samples. The Minute™ Plant ER Enrichment Kit was specially designed for plants. It can provide one- to threefold enrichment of the ER fraction from plant leaf samples in about one hour. The enriched ER is essentially free of Golgi apparatus contamination and can be used in studies of plant protein trafficking.

A. Ponceau S Stained Blot; B. Western blot with Anti-ER marker antibody (BIP2, Agrisera, Vannas, Sweden. AS 09481). Lanes 1&2: B. napus; Lanes 3&4: N. tabacum; Lanes 5&6: A. thaliana. Lanes 1, 3 & 5: Total Protein; Lanes 2, 4 & 6: ER fractions.

Kit Components: