Minute™ Chloroplast Isolation kit for Mucilaginous Plants (20 preps)

 Manual & Protocol | MSDS

Many crop species across the plant family contain high levels of polysaccharides and polyphenols. The hydrocolloid mucilage is a complex mucopolysaccharide typically showing high viscosity when released in solution. Homogenates of high-mucopolysaccharide plant samples usually impart a slimy consistency that contaminates cell fractionation and organelle purification, such as chloroplast and nuclear isolation. This kit provides a simple, rapid, and efficient method for isolating chloroplasts from viscous plant homogenate. The protocol can be done in 30 minutes. 

Figure 1. Chloroplasts isolated from leaves of indicated plant species.

Strawberry                                                     Plum     

Sweet Potato                                         Water Spinach

Figure 2.  Morphologies of chloroplasts isolated from leaves of indicated plants under light microscopy.

Kit Components: