Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps)

Manual & Protocol | MSDS

Invent Biotechnologies MinuteTM mitochondria isolation kit is composed of optimized buffers and protein extraction filter cartridges with 2.0 mL collection tubes. The kit is designed to rapidly isolate intact native mitochondria from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation procedure is simple, easy, and user-friendly, with high yield. Unlike other commercial mitochondrial preparation kits, this kit offers a wide range of starting cells (5-40 million/sample). The buffers are detergent- and EDTA-free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in about 30 min.

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute™ Mitochondria Isolation Kit. 

 A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues, respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues, respectively.

 B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048, a nuclear envelope marker protein. abcam, Cambridge, MA). 

 C. Densitometry measurement of mitochondrial marker signals.

Kit includes: