Catalog Number: ER-035
Manual & Protocol | MSDS
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation. The whole protocol can be done in about 2 hours.
Quantity
Buffer A
20 ml
Buffer B
4 ml
Buffer C
Plastic Rods
2 units
Filter Cartridge
20 units
Collection Tubes
Minute™ Plasma Membrane/Protein Isolation and Cell Fractionation Kit (50 Preps)
Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)
Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)
Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)
Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)
