Catalog Number: SC-003
Manual & Protocol | Material Safety Data Sheets (MSDS)
Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells comprises optimized cytoplasmic extraction buffer, nuclear protein extraction buffer, and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plants, bacteria, yeast, and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.
Kit includes:
Items
Quantity
Cytoplasmic Extraction Buffer
25 ml
Nuclear Extraction Buffer
Protein Extraction Filter Cartridges
50 units
Collection Tubes with Caps
Huang, H., Zhu, X., Cheng, H., Kuang, X., Long, C., Deng, X., ... & Shen, H. 2, 3, 5, 6-Tetramethylpyrazine protects retinal photoreceptors against endoplasmic reticulum stress by modulating ATF4-mediated inhibition of PRP aggregation. Journal of Molecular Medicine, 1-20.
Fang, M., Wu, H. K., Pei, Y., Zhang, Y., Gao, X., He, Y., ... & Xiao, R. P. (2023). E3 ligase MG53 suppresses tumor growth by degrading cyclin D1. Signal Transduction and Targeted Therapy, 8(1), 263.
Duan, C., Liu, R., Kuang, L., Zhang, Z., Hou, D., Zheng, D., ... & Li, T. (2023). Activated Drp1 Initiates the Formation of Endoplasmic Reticulum‐Mitochondrial Contacts via Shrm4‐Mediated Actin Bundling. Advanced Science, 2304885.
Wang, X., Xu, J., Li, Q., Zhang, Y., Lin, Z., Zhai, X., ... & Zheng, Y. (2023). RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor. Cancer Letters, 216486.
Cui, S., Wang, Y., Chen, S., Fang, L., Jiang, Y., Pang, Z., ... & Jia, H. (2023). African swine fever virus E120R inhibited cGAS‐STING‐mediated IFN‐β and NF‐κB pathways. Animal Research and One Health.LY6K.
Zhu, L., Xie, Z., Yang, G., Zhou, G., Li, L., & Zhang, S. (2023). Stanniocalcin-1 Promotes PARP1-Dependent Cell Death via JNK Activation in Colitis. Advanced Science, 2304123.
Wang, Y., Zhao, M., Cui, J., Lian, H., Hao, Z., Lou, L., ... & Zhang, X. (2024). Ochratoxin A-enhanced glycolysis induces inflammatory responses in human gastric epithelium cells through mTOR/HIF-1α signaling pathway. Ecotoxicology and Environmental Safety, 270, 115868.
Fu, P., Zhu, R., Gao, W., & Gong, L. (2023). Effects of resistance training on alleviating hypoxia‐induced muscle atrophy: Focus on acetylation of FoxO1. Journal of Cellular and Molecular Medicine.
Macejova, D., Kollar, J., Bobal, P., Otevrel, J., Schuster, D., & Brtko, J. (2024). Triphenyltin isoselenocyanate: a novel nuclear retinoid X receptor ligand with antiproliferative and cytotoxic properties in cell lines derived from human breast cancer. Molecular and Cellular Biochemistry, 1-16.
Li, L. B., Yang, L. X., Liu, L., Liu, F. R., Li, A. H., Zhu, Y. L., ... & Zhao, X. G. (2024). Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer. Acta Pharmacologica Sinica, 1-17.
Nishiguch, Y., Fujiwara-Tani, R., Nukaga, S., Nishida, R., Ikemoto, A., Sasaki, R., ... & Kuniyasu, H. (2024). Pterostilbene Induces Apoptosis from Endoplasmic Reticulum Stress Synergistically with Anticancer Drugs That Deposit Iron in Mitochondria. International Journal of Molecular Sciences, 25(5), 2611.
Hou, D., Liu, R., Hao, S., Dou, Y., Chen, G., Liu, L., ... & Duan, C. (2024). Notoginsenoside R1 improves intestinal microvascular functioning in sepsis by targeting Drp1-mediated mitochondrial quality imbalance. Pharmaceutical Biology, 62(1), 250-260.
Yang, W., Wei, Y., Wang, T., Xu, Y., Jin, X., Qian, H., ... & He, S. (2024). Cytoplasmic localization of SETDB1‑induced Warburg effect via c‑MYC‑LDHA axis enhances migration and invasion in breast carcinoma. International Journal of Molecular Medicine, 53(4), 1-15.
Li, Z., Mao, K., Liu, L., Xu, S., Zeng, M., Fu, Y., ... & Cheng, Y. (2024). Nuclear microRNA-mediated transcriptional control determines adult microglial homeostasis and brain function. Cell Reports, 43(3).
Wang, Z., Han, B., Qi, J., Cao, X., Gu, H., & Sun, J. (2024). Chuanzhitongluo capsule improves cognitive impairment in mice with chronic cerebral hypoperfusion via the cholinergic anti-inflammatory pathway. Experimental Gerontology, 189, 112407.
Hou, D., Liao, H., Hao, S., Liu, R., Huang, H., & Duan, C. (2024). Curcumin simultaneously improves mitochondrial dynamics and myocardial cell bioenergy after sepsis via the SIRT1-DRP1/PGC-1α pathway. Heliyon.
Fujiwara-Tani, R., Sasaki, T., Bhawal, U. K., Mori, S., Ogata, R., Sasaki, R., ... & Kuniyasu, H. (2024). Nuclear MAST4 Suppresses FOXO3 through Interaction with AKT3 and Induces Chemoresistance in Pancreatic Ductal Carcinoma. International Journal of Molecular Sciences, 25(7), 4056.
Li, X., Guan, W., Liu, H., Yuan, J., Wang, F., Guan, B., ... & Zhang, L. (2024). Targeting PNPO to suppress tumor growth via inhibiting autophagic flux and to reverse paclitaxel resistance in ovarian cancer. Apoptosis, 1-18.
Du, Y., Wu, M., Song, S., Bian, Y., & Shi, Y. (2024). TXNIP deficiency attenuates renal fibrosis by modulating mTORC1/TFEB-mediated autophagy in diabetic kidney disease. Renal Failure, 46(1), 2338933.
Wu, S., Huang, J., Li, Y., & Zhao, L. (2024). Comparative transcriptomics combined with physiological and functional analysis reveals the regulatory mechanism of rainbow trout (Oncorhynchus mykiss) under acute hypoxia stress. Ecotoxicology and Environmental Safety, 278, 116347.
Wang, L., Sun, W., Ren, G., Sun, Y., Xu, C., Song, Q., ... & Liu, Z. (2024). Deletion of Nrf2 induced severe oxidative stress and apoptosis in mice model of diabetic bladder dysfunction. International Urology and Nephrology, 1-10.
242. Wu, S., Huang, J., & Li, Y. (2024). A novel hypoxic lncRNA, LOC110520012 sponges miR-206-y to regulate angiogenesis and liver cell proliferation in rainbow trout (Oncorhynchus mykiss) by targeting vegfaa. Ecotoxicology and Environmental Safety, 280, 116554. (RNA)
243. Wang, Y., Chen, Y., Wu, J., & Shi, X. (2024). BMP1 Promotes Keloid by Inducing Fibroblast Inflammation and Fibrogenesis. Journal of Cellular Biochemistry, e30609.
244. Sun, T., Huang, J., Li, Y., Wu, S., Zhao, L., & Kang, Y. (2024). Identification and characterization of circular RNAs in the skin of rainbow trout (Oncorhynchus mykiss) infected with infectious hematopoietic necrosis virus. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 101277.
245. Wu, Y., Yang, Y., Qin, X., Zhang, Z., Ullah, M., Li, Y., & Zhang, Z. (2024). Unfolded proteins in the mitochondria activate HRI and shut-down of mitochondrial protein translation.
246. Wang, J., Wang, Y., Zhu, J., Wang, L., Huang, Y., Zhang, H., ... & Li, X. (2024). Promoter hypermethylation-induced downregulation of ITGA7 promotes colorectal cancer proliferation and migration by activating the PI3K/AKT/NF-κB pathway. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 119785.
247. Roy, M. E., Veilleux, C., Paquin, A., Gagnon, A., & Annabi, B. (2024). Transcriptional regulation of CYR61 and CTGF by LM98: a synthetic YAP-TEAD inhibitor that targets in-vitro vasculogenic mimicry in glioblastoma cells. Anti-Cancer Drugs, 10-1097.
248. Arthur, N. B., Christensen, K. A., Mannino, K., Ruzinova, M. B., Kumar, A., Gruszczynska, A., ... & Ferraro, F. (2024). Missense Mutations in Myc Box I Influence Nucleocytoplasmic Transport to Promote Leukemogenesis. Clinical Cancer Research, OF1-OF18.
249. Li, X., Tian, B. M., Yin, Y., Zhou, H., Gan, D., Liu, F., ... & Chen, F. M. (2024). Melatonin Ameliorates Inflammation-induced Mitochondrial Dysfunction and Cementoblastic Differentiation in Cells by Regulating the METTL3/LINC01444/HSPD1 Axis. Fundamental Research.
250. Wu, C. J., Livak, F., & Ashwell, J. D. (2024). The histone methyltransferase KMT2D maintains cellular glucocorticoid-responsiveness by shielding the glucocorticoid receptor from degradation. Journal of Biological Chemistry, 107581.
251. Snider, P. L., Sierra Potchanant, E. A., Sun, Z., Edwards, D. M., Chan, K. K., Matias, C., ... & Conway, S. J. (2024). A Barth Syndrome Patient-Derived D75H Point Mutation in TAFAZZIN Drives Progressive Cardiomyopathy in Mice. International Journal of Molecular Sciences, 25(15), 8201.
252. Kuramoto, K., Morishima, Y., Yoshida, K., Ano, S., Kawashima, K., Yabuuchi, Y., ... & Hizawa, N. (2024). Nrf2 Deficiency Accelerates IL-17-Dependent Neutrophilic Airway Inflammation in Asthmatic Mice. Antioxidants, 13(7).
253. Chang, Y. F., Huang, Y. P., Chou, C. H., Ho, M. W., Lin, H. J., Chen, C. Y., ... & Lai, C. H. (2024). RAGE participates in the intracellular transport of Campylobacter jejuni cytolethal distending toxin. Journal of Microbiology, Immunology and Infection.
254. Yu, H., Huang, L., Gui, L., Wu, Z., Luo, H., Xu, M., ... & Li, F. (2024). Luteolin ameliorates hyperuricemic nephropathy by activating urate excretion and Nrf2/HO‐1/NQO1 antioxidant pathways in mice. Food Science & Nutrition.
255. Tang, J., Tie, X., Zhi, S., Wang, Z., Zhao, Q., Qu, Z., ... & Shi, Y. (2024). Discovery of novel 5-phenyl-1H-pyrrole-2-carboxylic acids as Keap1-Nrf2 inhibitors for acute lung injury treatment. Bioorganic Chemistry, 107741.
256. Roy, M. E., Veilleux, C., & Annabi, B. (2024). In vitro biomaterial priming of human mesenchymal stromal/stem cells: implication of the Src/JAK/STAT3 pathway in vasculogenic mimicry. Scientific Reports, 14(1), 21444.
257. Zhao, L., An, Y., Zhao, N., Gao, H., Zhang, W., Gong, Z., ... & Zhao, Q. (2024). Spatially resolved profiling of protein conformation and interactions by biocompatible chemical cross-linking in living cells. Nature Communications, 15(1), 8331.
258. Yuuri Take, Fumi Yoshida, Ryo Tanaka, Yusuke Nakanishi, Kyoko Takahashi, Epidermal growth factor induces the initiation of epithelial mesenchymal transition in high-density colorectal cancer cell culture, Bioscience, Biotechnology, and Biochemistry, 2024.
259. Zhou, M., Li, Y., Yang, L., Liu, S., Yang, L., Xu, B., ... & Song, Z. (2024). LncRNA PTPRG-AS1 Promotes Breast Cancer Progression by Modulating the miR-4659a-3p/QPCT Axis. OncoTargets and Therapy, 805-819.
260. He, S., Huang, L., Chen, Z., Yuan, Z., Zhao, Y., Zeng, L., ... & He, J. (2024). Disruption of the Novel Nested Gene Aff3ir Mediates Disturbed Flow-Induced Atherosclerosis in Mice. bioRxiv, 2024-10.
261. Xia, T., Meng, L., Xu, G., Sun, H., & Chen, H. (2024). TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth. Cell Death & Disease, 15(10), 740.
262. Shofa, M., Fukushima, Y. V., & Saito, A. (2024). Conserved yet Divergent Smc5/6 Complex Degradation by Mammalian Hepatitis B Virus X Proteins. bioRxiv, 2024-10.
Minute™ Plasma Membrane/Protein Isolation and Cell Fractionation Kit (50 Preps)
Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)
Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)
Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps)