Comparison of Major Lipid Raft Isolation Kits
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Lipid rafts are important subcellular structures rich in cholesterol and sphingolipid and signaling proteins. Lipid rafts play a critical role in cellular transport and signal transduction. The traditional method of lipid raft isolation is tedious and time-consuming. There are limited numbers of commercial kits for the isolation of this important structure.
| Brand/manufacturer | Brand# 1 | Invent Biotech |
|---|---|---|
| Product Name | Caveolae/Rafts Isolation Kit | MinuteTM Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues |
| Mechanism of Isolation | Ultracentrifugation flotation | Spin column-based - floatation with a tabletop centrifuge |
| Average sample size | Cells (20-50 million) | Tissue (35 mg) Cells (20-30 million) |
| Number of buffers and reagents used | 6 | 3 |
| Buffer preparation prior to use | Required | Not required |
| Homogenizer | Required | Not required |
| Density gradient formation | Not Required | Not required |
| Ultracentrifugation | Not Required | Not required |
| Purity of Isolated Lipid Rafts | Total Detergent Resistant lipid Rafts | Total Detergent Resistant Lipid Rafts |
| Approximate Protocol Time | >5 h | 1h |