Comparison of Major ER Isolation Kits
Download as a pdfThe endoplasmic reticulum (ER) is a membranous labyrinth extending throughout the cell. The ER is a major organelle and accounts for about 1/2 of total cellular membrane. It plays a critical role in protein synthesis and modification. There are a few commercial kits available for ER isolation with different mechanisms of actions.
| Brand/manufacturer | Brand# 1 | Brand# 2 | Invent Biotech |
|---|---|---|---|
| Product Name | ER Enrichment Kit | Endoplasmic Reticulum Isolation Kit | MinuteTM ER Enrichment Kit |
| Mechanism of Isolation | Differential centrifugation and precipitation | Density gradient centrifugation | Spin column-based -precipitation |
| Average sample size | Tissue (500 mg) | Tissue (3-7 g) Cells (2000 million) | Tissue (35 mg) Cells (30 million) |
| Number of buffers and reagents used | 5 | 4 | 4 |
| Buffer preparation prior to use | Required | Required | Not required |
| Homogenizer | Required | Required | Not required |
| Density gradient formation | Not Required | Required | Not required |
| Ultracentrifugation | Not Required | Required | Not required |
| Purity of Isolated ER | Enriched ER | Enriched ER | Enriched ER |
| Approximate Protocol Time | 1-2h | 1.5-5h | 1.5h |