{"title":"Popular Products","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eExplore Invent Biotechnologies’ most popular products, trusted by researchers for fast, reliable, and reproducible sample preparation. This collection highlights frequently selected kits across protein extraction, cell fractionation, organelle isolation, and single nuclei preparation.\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003ca href=\"https:\/\/inventbiotech.com\/collections\/all-products\"\u003e\u003cspan style=\"color: rgb(255, 128, 0);\"\u003e\u003cstrong\u003e\u003cspan style=\"text-decoration: underline;\"\u003eView all Products →\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"minute-total-protein-extraction-kit-for-animal-cultured-cells-and-tissues-us-patent-9-580-462","title":"Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)","description":"\u003ch2\u003e\n\u003cspan style=\"font-weight: 400;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/1-Sd-001-SN-002_v8.pdf?v=1752160057\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1.pdf?12490201953603370455\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe height=\"315\" width=\"560\" src=\"https:\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" title=\"YouTube video player\"\u003e\u003c\/iframe\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/news-1\/ripa-buffer-the-potential-troubles\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003ca href=\"https:\/\/inventbiotech.com\/pages\/ripa-buffer-the-potential-troubles\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Cell_Sensitization_1.5_x_1.5_in.png?v=1752241598\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"110\" height=\"110\"\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cp\u003eThe Minute™ kit is a next-generation, spin column–based system designed for rapid and efficient total protein extraction from animal cultured cells and tissues. Unlike conventional solution-based methods, such as RIPA buffer, which often result in protein loss and altered profiles, this patented technology preserves protein integrity and yields reproducible results.\u003c\/p\u003e\n\u003cp\u003eWith enhanced lysis buffers and a streamlined protocol, proteins can be extracted in just 1–5 minutes at concentrations ranging from 1–8 mg\/ml. The system supports both denaturing and native conditions, allowing users to choose based on downstream applications. Minimal extraction volume (as low as 20 µl) makes it ideal when starting material is limited.\u003c\/p\u003e\n\u003cp\u003eThis kit offers one of the fastest protocols available for total protein preparation and can be readily adapted for high-throughput workflows. The extracted proteins are also suitable as starting material for small-scale purification using column chromatography.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/ripa-buffer-the-potential-troubles-1\"\u003e-RIPA Buffer - The Potential Troubles\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/pitfalls-of-protein-extraction-by-ripa-buffer-1\"\u003e-Pitfalls of Protein Extraction by RIPA\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/news-1\/a-technical-breakthrough-for-protein-sample-preparation\"\u003e-A Technical Breakthrough for Protein Sample Preparation\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eSelected references related to protein loss by RIPA buffer:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eBai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048\u003c\/li\u003e\n\u003cli\u003eMukhopadhyay, C. et al. (2016) PNAS 5:8228-8237\u003c\/li\u003e\n\u003cli\u003eLi, Q. (2016) Biotechniques. 61:327\u003c\/li\u003e\n\u003cli\u003eNgoka, L. CM. (2008) Proteome Science. 6:30\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_A_3600ed44-960b-492c-9588-9166e605dcf6_large.jpg?v=1540501877\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_B_12cc4294-e3c3-44fd-9dc4-b06d38768b63_large.jpg?v=1540501895\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003eProtein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver. \u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_D_aea4bcc2-c65b-4095-a711-cc8f429e95cd_large.jpg?v=1540501948\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_Fish-GPDH-F_copy.jpg?836\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/RIPA_pellet_large.jpg?v=1555514187\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eKit includes:\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eItems\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eQuantity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Cell Lysis Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Cell Lysis Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: SD-001\/SN-002","offers":[{"title":"50 Preps","offer_id":16720730488943,"sku":"Cat #: SD-001\/SN-002","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SD-001_SN-002_1024x1024_4f04fd1f-0448-4d47-a2d7-7547a3477a81.png?v=1569161589"},{"product_id":"minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes","title":"Minute™ Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/22-AT-022_v10.pdf?v=1650999022\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_435fc4ec-c7e0-4ad8-a74f-5f8537e5d08b.pdf?14248766210075683969\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eAdipose tissue, especially white adipose tissue (WAT), has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. Analyses of proteins from adipose tissues are increasingly critical for understanding many physiological\/pathological conditions. However, isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents. The water-oil emulsion present in a biological sample is notoriously difficult to separate. We have developed a novel technology to address this issue. A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effectively separate water-oil emulsion derived from adipose tissue homogenate. The extraction buffer has a lower freezing point than oil in adipose tissues, and the aqueous phase can be quickly separated from the oil phase by passing the tissue homogenate through the filter. The total proteins isolated are the unbiased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg\/ml) compared to traditional methods.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/protein-extraction-from-adipose-tissues-a-tough-challenge-1\" title=\"Protein Extraction from Adipose Tissues--- A Tough Challenge\" style=\"color: #ff8000;\" target=\"_blank\"\u003eProtein Extraction from Adipose Tissues--- A Tough Challenge\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/high-efficiency-protein-extraction-from-adipose-tissues-1\" title=\"High Efficiency Protein Extraction from Adipose Tissues\" style=\"color: #ff8000;\" target=\"_blank\"\u003eHigh-Efficiency Protein Extraction from Adipose Tissues\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/novel-method-for-high-quality-protein-extraction-from-adipose-tissues\" title=\"Novel Method for High Quality Protein Extraction from Adipose Tissues\" style=\"color: #ff8000;\" target=\"_blank\"\u003eNovel Method for High-Quality Protein Extraction from Adipose Tissues\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cimg style=\"font-size: 0.875rem;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AT-022_A_V2_large.jpg?v=1540918103\"\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AT-022_B_76908621-045f-4fe4-802d-81ae6c979dd5_large.jpg?v=1540504600\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA. SDS-PAGE (10%) profiles of total protein extracted from different adipose tissues. Lane 1, porcine WAT; Lane 2, chicken BAT; Lane 3, rat WAT.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eB. Western blottings of extracted proteins from rat WAT. Proteins were separated in 8-16% gradient SDS-PAGE and probed with following cellular protein marker antibodies:\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003eAnti-Na\/K ATPase alpha1, a plasma membrane marker (Upstate, clone 464.6), anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA), anti-ubiquinol-cytochrome C reductase core protein (abcam, ab 96333) and GAPDH, a cytosolic marker (Sigma). The specific protein bands were visualized by a substrate Opti-4CN (Bio-RAD).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Kit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable height=\"325\" width=\"540\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"text-align: center; height: 33px; width: 424px;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eBuffer A (Extraction Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003eBuffer B (10 X Denaturing Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003eBuffer C (10 X Non-Denaturing Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003e1.5 ml Microfuge Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21.3542px;\"\u003e\n\u003ctd style=\"height: 21.3542px; width: 424px;\"\u003e\n\u003cp\u003ePestles for 1.5 ml Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 21.3542px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 32px;\"\u003e\n\u003ctd style=\"height: 32px; width: 424px;\"\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 32px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 32px;\"\u003e\n\u003ctd style=\"height: 32px; width: 424px;\"\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 32px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: AT-022","offers":[{"title":"20 Preps","offer_id":16745493528687,"sku":"Cat #: AT-022","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/AT-022_1024x1024_7f058af1-f077-4669-86a5-79d0f6888557.png?v=1550164740"},{"product_id":"minute-plasma-membrane-protein-isolation-and-cell-fractionation-kit","title":"Minute™ Plasma Membrane\/Protein Isolation and Cell Fractionation Kit (50 Preps)","description":"\u003ch2\u003e\n\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5-SM-005_V11.pdf?v=1750697274\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003c\/strong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_32b5f5b1-bc6f-4d57-8f53-173b07067285.pdf?881483754317527433\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003cbr\u003e\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFree of detergents and EDTA, the Minute™ kit is a next-generation solution for native plasma membrane (PM) isolation and cell fractionation. It offers exceptional convenience and consistency by eliminating the variability often seen with traditional methods such as homogenization, density gradient centrifugation, and two-phase partitioning.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e\u003cspan style=\"color: #e69138;\"\u003eHow it works:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/em\u003e cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied, resulting in a cell lysate containing ruptured cell membranes and intact nuclei. As a result, nuclear contamination is virtually eliminated. PM is further separated from the cell lysate (a mixture of crude membranes, intact nuclei, cytosol proteins, and organelles) by subsequent differential and density centrifugation with a regular tabletop microcentrifuge. 5 distinct cell fractions (total membrane, PM, cytosol, nucleus, and organelles) can be obtained. The procedure can be completed in less than 45 minutes.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca style=\"color: #ff8000;\" href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/how-to-choose-your-membrane-protein-isolation-kits?_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"How to Choose Membrane Protein Isolation Kits\" target=\"_blank\"\u003eHow to Choose Membrane Protein Isolation Kits\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg height=\"408\" width=\"544\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/2_815b9e95-7f48-4418-95e1-eee070746034_1024x1024.png?v=1638553149\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_A.jpg?v=1613705277\"\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eFigure 1. A. SDS-PAGE profiles of total cell lysate (TC) and isolated plasma membrane proteins (PM) from human and rat cultured cells. Lane 1, Human lung cancer cell A549 total cell lysate; Lane 2, Isolated PM of A549; Lane 3, PM of rat REL-6TN cells; Lane 4, Total cell lysate of rat REL-6TN cells.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Western blotting: Proteins in A were transferred to nitrocellulose membrane and probed with anti-Na\/K ATPase alpha1, a plasma membrane marker (Upstate, Clone 464.6), and anti-lamin B1, a nuclear envelope marker (ab16048, Abcam Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD).\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eC. Densitometry measurement of Na\/K ATPase alpha1 signals in B (TC vs. PM). Total cell lysates were extracted by Minute Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_B.jpg?v=1613705277\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 2 A. SDS-PAGE profiles of isolated total membrane proteins from mouse tissues (T=Total Cell Lysate, C=Cytosol Fraction, M= Total Membrane Fraction)\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Proteins shown in A were transferred to a nitrocellulose membrane and probed with rabbit-anti mouse pan-cadherin (ab6529, Abcam, Cambridge, MA), and anti-actin by Western blotting. The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). Signals of pan-cadherin (a 130 kDa plasma membrane marker) were significantly enhanced in total membrane protein fractions. Total cell lysates were extracted by Minute™ Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Plymouth, MN).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_C.jpg?v=1613705277\"\u003eFigure 3. A. SDS-PAGE profiles of total membrane protein fraction (TM) and isolated plasma membrane protein fraction from mouse tissues. \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Western blotting of proteins in A were transferred to nitrocellulose membrane and probed with rabbit anti-cadherin (abcam, Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eC. Densitometry measurement of cadherin signals in B (TM vs. PM).\u003c\/p\u003e\n\u003cp\u003e  \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: SM-005","offers":[{"title":"50 Preps","offer_id":16745644097647,"sku":"Cat #: SM-005","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SM-005_1024x1024_a26abe5b-9b42-400f-87e8-71c928c68b05.png?v=1569162641"},{"product_id":"minute-plasma-membrane-protein-isolation-kit-for-plants","title":"Minute™ Plant Plasma Membrane\/Protein Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5p-SM-005p_v4.pdf?v=1613417660\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_47c0cabc-5d71-4cb6-ad4c-c17eaf1cf725.pdf?18003124456286137432\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning. These relatively effective methods require ultracentrifugation and a large amount of starting material. The procedures are usually tedious and time-consuming. To overcome the shortcomings, we have developed this PM isolation kit. Plant tissues are first sensitized by buffer A, homogenized and then passed through a specialized filter cartridge that allows homogenates to pass through with a zigzag path. The cell membranes are ruptured into a range of predefined sizes during the process. Native plasma membranes are separated from a mixture of unruptured cells, nuclei, cytosol and organelles by subsequent differential centrifugation and density centrifugation without using ultracentrifugation. Due to the use of the same amount of starting material, defined centrifugal force and predefined duration in every experiment, the result is much more consistent with a high degree of PM protein enrichment. The procedure can be completed in about 1 hour.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SM-005-P","offers":[{"title":"50 Preps","offer_id":16745966567535,"sku":"Cat #: SM-005-P","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SM-005-P_1024x1024_6b89c76f-4411-40f9-8512-6bd5bf1500c2.png?v=1540418227"},{"product_id":"minute-endosome-isolation-and-cell-fractionation-kit","title":"Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/28-ED-028_v5.pdf?v=1709751079\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_de7ff84c-0ca7-4b35-8b8d-a79f38edbec4.pdf?10677628874286709938\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eEarly endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/ED-028_large.jpg?v=1540560730\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2.5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: ED-028","offers":[{"title":"20 Preps","offer_id":16746299785327,"sku":"Cat #: ED-028","price":730.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/ED-028_1024x1024_da5b4e82-26f2-40b0-815f-b1f71eee32d7.png?v=1540420183"},{"product_id":"minute™-nuclei-and-cytosol-isolation-kit-for-adipose-tissues","title":"Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/29-AN-029_v10.pdf?v=1650999549\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_91a88af6-f3fe-47d0-b535-4810c3254e35.pdf?9884351379084524198\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e\u003cspan style=\"color: #410f0f;\"\u003e*Invent Biotechnologies, Inc. is the inventor of the\u003cspan\u003e \u003c\/span\u003espin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the\u003cspan\u003e \u003c\/span\u003etechnology.\u003c\/span\u003e \u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eAdipocytes are the major energy storage sites in the body, and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into a single nucleus and cytosolic fraction is a common lab practice. However, when it comes to adipose tissue and adipocytes, separating these two fractions is quite challenging due to the low protein content and the presence of abundant lipid droplets. Methods developed by different labs are tedious and unpractical, requiring up to 50 grams of starting materials. This kit provides a quick and simple protocol for obtaining high-quality single nuclei and cytosol fractions from adipose tissue and adipocytes. Only milligrams of starting material are required, making it possible to isolate nuclei and extract proteins from biopsies and limited tissues. The intact nuclei isolated can be used in many applications such as scRNA-seq, ATAC-seq, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, and other applications.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFor storage and transportation of isolated single nuclei, the following buffer is recommended:\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1\u0026amp;_sid=8b67760c6\u0026amp;_ss=r\"\u003e\u003cem\u003eWA-014: Minute™ Anti-Clumping Nuclei Storage Buffer \u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWe also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute-detergent-free-nuclei-isolation-kit?_pos=1\u0026amp;_sid=844017157\u0026amp;_ss=r\"\u003eNI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps?_pos=1\u0026amp;_sid=1f449fc5a\u0026amp;_ss=r\" target=\"_blank\"\u003eBN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues\/Cells\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1\u0026amp;_sid=b147abdb9\u0026amp;_ss=r\"\u003e\u003cem\u003eSN-047: Minute™ Single Nucleus Isolation Kit for Tissues\/Cells\u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AN-029_A_V2_large.jpg?v=1540918331\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AN-029_B_large.jpg?v=1540560824\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eN\/C Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1.5 ml Eppendorf Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestle for 1.5 ml Eppendorf Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2 ml Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (2)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eRajbhandari, P., Arneson, D., Feng, A. C., Ahn, I. S., Diamante, G., Zaghari, N., ... \u0026amp; Smale, S. T. (2019). Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes. \u003cem data-mce-fragment=\"1\"\u003eeLife 2019;8:e49501.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"mso-bidi-font-size: 12.0pt; line-height: 107%; mso-ascii-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri; mso-bidi-font-family: Calibri; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e   \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003eFelemban, A. H., Alshammari, G. M., Yagoub, A. E. A., Saleh, A., \u0026amp; Yahya, M. A. (2024). Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing Adipogenesis. \u003ci\u003eNutrients\u003c\/i\u003e, \u003ci\u003e16\u003c\/i\u003e(18), 3174.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: AN-029","offers":[{"title":"20 Preps","offer_id":16746318299247,"sku":"Cat #: AN-029","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/AN-029_1024x1024_196036db-25b2-4cf8-99d4-9aab7e67e2f0.png?v=1569162674"},{"product_id":"minute™-lysosome-isolation-kit-for-mammalian-cells-tissues","title":"Minute™ Lysosome Isolation Kit for Mammalian Cells\/Tissues (20 Preps)","description":"\u003ch2 class=\"p1\"\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/34-LY-034_v5.pdf?v=1709826888\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?325\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e \u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eLysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes crucial for breaking down excess or worn-out organelles, food particles, as well as viruses and bacteria that have been engulfed. Lysosomes exhibit a relatively large size, ranging from 0.1 to 1.2 micrometers. In the realm of cell research, the initial isolation of lysosomes is a pivotal step when investigating processes like autophagy, protein degradation, and recycling.\u003c\/p\u003e\n\u003cp\u003eTraditional methods for lysosome isolation, which originated in the 1970s, are associated with challenges such as the need for substantial starting material and a time-consuming procedure that often leads to significant cross-contaminations. However, a groundbreaking solution is offered by this kit, utilizing patented spin-column-based technology that is characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be notably enriched without the requirement of a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/comparison-of-major-lysosome-isolation-kits-1\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003eCompare with Other Lysosome Isolation Kits\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_A_1bdfa113-6b92-4a86-b09a-517f73e42ab7_large.jpg?v=1541108023\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_bargraph_A_large.png?v=1541108107\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_bargraph_B_large.png?v=1541108120\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_B_large.jpg?v=1541108040\"\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 544.891px;\" height=\"204\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px; text-align: center;\"\u003e2 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: LY-034","offers":[{"title":"20 Preps","offer_id":17154144338031,"sku":"Cat #: LY-034","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/LY-034_1024x1024_d1be844a-d953-423e-b655-4434c36b7943.png?v=1541099748"},{"product_id":"minute™-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps","title":"Minute™ Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/20-BN-20_v6.pdf?v=1747074289\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_be29e4a0-2c3c-4817-ba9a-12f41212d03a.pdf?867\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e\u003cspan style=\"color: #410f0f;\"\u003e*Invent Biotechnologies, Inc. is the inventor of the\u003cspan\u003e \u003c\/span\u003espin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the\u003cspan\u003e \u003c\/span\u003etechnology.\u003c\/span\u003e \u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eIsolated nuclei are widely used for various experiments such as FACS, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle, and apoptosis research. Single-cell RNA-seq is a powerful technology for studying the complex cellular composition of tissues. However, Neurons are highly interconnected, and it is challenging to obtain single cells from neuronal tissues such as the brain and spinal cord. It is even more difficult to isolate intact cells from frozen neuronal tissues. Due to these limitations, single-cell RNA-seq is being substituted by single-nucleus-seq. The traditional method for single nucleus isolation from neuronal tissue is relatively tedious and time-consuming, and the yield is usually low because it is difficult to get rid of contaminating myelin and other cellular components. This kit is designed to address the issues with a simple, rapid, and effective protocol. The highly purified single nucleus can be obtained in about 30 min. Compared to the traditional method, the kit requires much less starting material and can handle an extensive range of sample sizes (1-25 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not desirable, a detergent-free nuclei isolation kit is also available under \u003ca href=\"https:\/\/inventbiotech.com\/products\/minute-detergent-free-nuclei-isolation-kit?_pos=1\u0026amp;_sid=40c51492d\u0026amp;_ss=r\" target=\"_blank\"\u003eCat #: NI-024\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFor storage and transportation of isolated single nuclei, the following buffer is recommended:\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1\u0026amp;_sid=8b67760c6\u0026amp;_ss=r\"\u003e\u003cem\u003eWA-014: Minute™ Anti-Clumping Nuclei Storage Buffer \u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWe also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute-detergent-free-nuclei-isolation-kit?_pos=1\u0026amp;_sid=844017157\u0026amp;_ss=r\"\u003eNI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1\u0026amp;_sid=b147abdb9\u0026amp;_ss=r\"\u003e\u003cem\u003eSN-047: Minute™ Single Nucleus Isolation Kit for Tissues\/Cells\u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-nuclei-and-cytosol-isolation-kit-for-adipose-tissues?_pos=1\u0026amp;_sid=f91e6c921\u0026amp;_ss=r\" target=\"_blank\"\u003eAN-029: Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/BN-020-Zbfish_Brain_copy_480x480.jpg?v=1580321128\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNuclei Isolated from zebrafish brain tissue Using BN-020 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Université Libre de Bruxelles).\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Mouse-Brain_copy.jpg?865\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Pig-BF_copy.jpg?865\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Pig-BCord-F_copy.jpg?865\"\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Cleanup_data.jpg?865\"\u003e\u003c\/div\u003e\n\u003cp\u003eKit includes:\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestles for 1.5 ml Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: BN-020","offers":[{"title":"20 Preps","offer_id":31131264483439,"sku":"Cat #: BN-020","price":562.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/BN-020_a6e8614b-14f5-4c8c-a0b4-be30b9334fd9.png?v=1779381097"},{"product_id":"test-product","title":"Minute™ Test Plasma Membrane\/Protein Isolation and Cell Fractionation Kit","description":"\u003cstyle\u003e\n  .invent-product-links {\n    margin-bottom: 18px;\n    color: #182e49;\n  }\n\n  .invent-product-links a {\n    color: #182e49;\n    text-decoration: none;\n    font-weight: 700;\n  }\n\n  .invent-product-links a:hover {\n    color: #f6921e;\n    text-decoration: underline;\n  }\n\n  .invent-product-links .divider {\n    color: #f6921e;\n    padding: 0 8px;\n  }\n\n  .invent-product-description {\n    color: #182e49;\n  }\n\n  .invent-product-description details {\n    border: 1px solid #ddd;\n    border-radius: 6px;\n    margin: 12px 0;\n    background: #f5f5f5;\n    overflow: hidden;\n  }\n\n  .invent-product-description summary {\n    cursor: pointer;\n    padding: 14px 16px;\n    font-weight: 700;\n    color: #182e49;\n    background: #f5f5f5;\n    list-style: none;\n    position: relative;\n  }\n\n  .invent-product-description summary::-webkit-details-marker {\n    display: none;\n  }\n\n  .invent-product-description summary::after {\n    content: \"+\";\n    position: absolute;\n    right: 16px;\n    top: 50%;\n    transform: translateY(-50%);\n    color: #f6921e;\n    font-size: 24px;\n    font-weight: 700;\n  }\n\n  .invent-product-description details[open] summary::after {\n    content: \"–\";\n  }\n\n  .invent-product-description summary:hover {\n    color: #f6921e;\n  }\n\n  .invent-product-description details \u003e p,\n  .invent-product-description details \u003e ul,\n  .invent-product-description details \u003e div {\n    margin: 14px 16px;\n  }\n\n  .invent-product-description details \u003e table {\n    margin: 14px 16px;\n    width: calc(100% - 32px) !important;\n  }\n\n  .invent-product-description .invent-kit-table {\n    width: calc(100% - 32px) !important;\n    border-collapse: collapse !important;\n    background: #f5f5f5 !important;\n    border: 1px solid #d8d8d8 !important;\n  }\n\n  .invent-product-description .invent-kit-table tbody,\n  .invent-product-description .invent-kit-table tr {\n    background: transparent !important;\n  }\n\n  .invent-product-description .invent-kit-table td {\n    border: 1px solid #d8d8d8 !important;\n    padding: 10px !important;\n    background: #f2f2f2 !important;\n    color: #182e49 !important;\n  }\n\n  .invent-product-description .invent-kit-table tr:nth-child(odd):not(:first-child) td {\n    background: #eeeeee !important;\n  }\n\n  .invent-product-description .invent-kit-table tr:first-child td {\n    background: #182e49 !important;\n    color: #ffffff !important;\n    font-weight: 700 !important;\n  }\n\n  .invent-product-description img {\n    max-width: 100%;\n    height: auto;\n  }\n\u003c\/style\u003e\n\u003ch2 class=\"invent-product-links\"\u003e\n\u003cstrong\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5-SM-005_V11.pdf?v=1750697274\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan class=\"divider\"\u003e|\u003c\/span\u003e \u003c\/strong\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_32b5f5b1-bc6f-4d57-8f53-173b07067285.pdf?881483754317527433\" target=\"_blank\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e \u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"invent-product-description\"\u003e\n\u003cp\u003eThe Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit is designed for rapid, detergent-free, and EDTA-free isolation of native plasma membrane proteins from cultured cells and animal tissues. The kit uses Invent Biotechnologies’ proprietary spin-column technology to reduce variability associated with traditional homogenization, density gradient centrifugation, and two-phase partitioning methods.\u003c\/p\u003e\n\u003cp\u003eThe procedure can be completed in less than 45 minutes using a standard tabletop microcentrifuge and allows researchers to obtain five distinct fractions: total membrane, plasma membrane, cytosol, nucleus, and organelles.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDetergent-free and EDTA-free workflow\u003c\/li\u003e\n\u003cli\u003eIsolates native plasma membrane proteins\u003c\/li\u003e\n\u003cli\u003eProduces multiple cell fractions from the same sample\u003c\/li\u003e\n\u003cli\u003eDesigned to minimize cross-contamination\u003c\/li\u003e\n\u003cli\u003eNo ultracentrifugation required\u003c\/li\u003e\n\u003cli\u003eCompleted in less than 45 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow It Works\u003c\/summary\u003e\n\u003cp\u003eCells or tissues are first sensitized with Buffer A and then passed through the proprietary filter cartridge under high-speed centrifugal force. The sample moves through the filter in a zigzag manner, producing a lysate that contains ruptured cell membranes while leaving nuclei largely intact.\u003c\/p\u003e\n\u003cp\u003eBecause intact nuclei are separated early in the process, nuclear contamination is minimized. Plasma membrane proteins are then further separated from the lysate through subsequent differential and density centrifugation steps using a regular tabletop microcentrifuge.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eApplications\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003ePlasma membrane protein isolation\u003c\/li\u003e\n\u003cli\u003eNative membrane protein preparation\u003c\/li\u003e\n\u003cli\u003eCell fractionation studies\u003c\/li\u003e\n\u003cli\u003eMembrane marker analysis\u003c\/li\u003e\n\u003cli\u003eWestern blotting\u003c\/li\u003e\n\u003cli\u003eSDS-PAGE\u003c\/li\u003e\n\u003cli\u003eProtein localization studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eKit Contains\u003c\/summary\u003e\n\u003ctable class=\"invent-kit-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A\u003c\/td\u003e\n\u003ctd\u003e25 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer B\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtein Extraction Filter Cartridges\u003c\/td\u003e\n\u003ctd\u003e50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes with Caps\u003c\/td\u003e\n\u003ctd\u003e50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlastic Rods\u003c\/td\u003e\n\u003ctd\u003e2 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTissue Dissociation Beads\u003c\/td\u003e\n\u003ctd\u003e5 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eSupporting Data\u003c\/summary\u003e\n\u003cdiv\u003e\u003cimg alt=\"Plasma membrane isolation workflow\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/2_815b9e95-7f48-4418-95e1-eee070746034_1024x1024.png?v=1638553149\" width=\"544\" height=\"408\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_A.jpg?v=1613705277\" alt=\"SM-005 plasma membrane isolation data figure 1\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e SDS-PAGE and Western blot analysis of total cell lysate and isolated plasma membrane protein fractions from cultured human and rat cells. Plasma membrane enrichment was evaluated using Na\/K ATPase alpha1, while nuclear contamination was assessed using lamin B1.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_B.jpg?v=1613705277\" alt=\"SM-005 membrane protein isolation data figure 2\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e SDS-PAGE and Western blot analysis of total membrane proteins isolated from mouse tissues. Pan-cadherin, a plasma membrane marker, was enriched in the total membrane protein fraction.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_C.jpg?v=1613705277\" alt=\"SM-005 plasma membrane protein isolation data figure 3\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 3.\u003c\/strong\u003e SDS-PAGE and Western blot analysis comparing total membrane and plasma membrane protein fractions from mouse tissues. Cadherin signal enrichment was measured by densitometry.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003ch2\u003eRelated Articles\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca title=\"How to Choose Membrane Protein Isolation Kits\" href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/how-to-choose-your-membrane-protein-isolation-kits?_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eHow to Choose Membrane Protein Isolation Kits \u003c\/a\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Cat #: SM-005","offers":[{"title":"20 Preps","offer_id":53628655763769,"sku":"Cat #: SM-005","price":650.0,"currency_code":"USD","in_stock":true},{"title":"50 Preps - With total Protein Sample","offer_id":53628655796537,"sku":"Cat #: SM-005","price":1300.0,"currency_code":"USD","in_stock":true},{"title":"200 Preps","offer_id":53628655829305,"sku":"Cat #: SM-005","price":2500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_1024x1024_c118173a-eea7-4323-a91b-4f0c2eab2cf3.png?v=1782147114"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/collections\/Logo_maker_project-2_3.png?v=1752084322","url":"https:\/\/inventbiotech.com\/collections\/popular.oembed","provider":"Invent Biotechnologies Inc.","version":"1.0","type":"link"}