{"title":"Plant Products","description":"\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/pages\/black-friday-sale\" aria-label=\"Black Friday Sale\"\u003e\u003c\/a\u003eExplore our specialized line of tools designed specifically for plant research! Proprietary spin column–based technology enables the simple and efficient extraction of proteins and subcellular fractionation from plant tissues.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003ca href=\"https:\/\/inventbiotech.com\/collections\/all-products\"\u003e\u003cspan style=\"color: rgb(255, 128, 0);\"\u003e\u003cstrong\u003e\u003cspan style=\"text-decoration: underline;\"\u003eView all Products →\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"minute-total-protein-extraction-kit-for-plant-tissues","title":"Minute™ Total Protein Extraction Kit for Plant Tissues (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/8-SD-008-0SN-009_v5.pdf?v=1687456135\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_1ba26cd9-6f69-45bf-b9e9-ee267e712228.pdf?12885751298093300398\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Minute\u003csup\u003eTM\u003c\/sup\u003e total protein extraction kit for plant tissues comprises an optimized protein extraction buffer and protein extraction filter cartridges accompanied by 2.0 ml collection tubes. It aims to efficiently extract denatured or native proteins from various plant tissues, including leaves, seeds, soft stems, and roots. Notably, the protein profiles obtained through denaturing and native cell lysis buffers differ. Therefore, depending on the specific application, one buffer may outperform the other. This kit offers both denaturing and native cell lysis buffers, allowing users to evaluate and select the most suitable option for their particular needs. Utilizing the protein extraction filter cartridges makes it possible to extract total plant soluble proteins from 50-200 mg of plant tissue within 5-8 minutes while achieving a high protein yield ranging from 2-8 mg\/ml. This extraction method is useful for SDS-PAGE, immunoblotting, ELISA, IP, and enzyme assays. These extracted proteins can also be excellent starting materials for small-scale protein purification using column chromatography techniques.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-008_SN-009_large.jpg?v=1540565710\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eDenaturing Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eNative Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (340)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eCrystal R. et al. (2013). Expression profiling of four defense-related buffalograss transcripts in response to chinch bug (hemiptera: Blissidae) feeding. Journal of Economic Entomology. 106 (6):2568-2576.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWeber, C. F. (2017). Microgreen Farming and Nutrition: A Discovery-Based Laboratory Module to Cultivate Biological and Information Literacy in Undergraduates. The American Biology Teacher, 79(5), 375-386.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAbdulazeez, M., Abubakar, S. M., \u0026amp; Mu’azzam, J. M. (2017). Isolation and Characterization of a Potential Angiotensin-Converting Enzyme Inhibitory Peptide from the Leaves of Leptadenia hastata (Asclepiadaceae). Malaysian Journal of Applied Sciences, 2(1), 35-47.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShi, Z., Jiang, Y., Han, X., Liu, X., Cao, R., Qi, M., ... \u0026amp; Li, T. (2017). SlPIN1 regulates auxin efflux to affect flower abscission process. Scientific Reports, 7(1), 14919.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eOhno, S., Hori, W., Hosokawa, M., Tatsuzawa, F., \u0026amp; Doi, M. (2018). Post-transcriptional silencing of chalcone synthase is involved in phenotypic lability in petals and leaves of bicolor dahlia (Dahlia variabilis)‘Yuino’. Planta, 247(2), 413-428.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eJesus, F. G., Marchi-Werle, L., Fischer, H. D., Posadas, L. G., Graef, G. L., \u0026amp; Heng-Moss, T. Documenting Resistance and Physiological Changes in Soybean Challenged by Aphis glycines Matsumura (Hemiptera: Aphididae). Neotropical Entomology, 1-8.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLv, Z., Huang, Y., Ma, B., Xiang, Z., \u0026amp; He, N. (2018). LysM1 in MmLYK2 is a motif required for the interaction of MmLYP1 and MmLYK2 in the chitin signaling. Plant Cell Reports, 1-12.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMarchi-Werle, L., Fischer, H. D., Graef, G., Hunt, T. E., \u0026amp; Heng-Moss, T. M. (2018). Characterization and Identification of Methods for Phenotyping Soybean Populations With Tolerance to the Soybean Aphid (Hemiptera: Aphididae). Journal of Economic Entomology. toy177, https:\/\/doi.org\/10.1093\/jee\/toy177\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWang, J., Xia, H., Zhao, S. Z., Hou, L., Zhao, C. Z., Ma, C. L., ... \u0026amp; Li, P. C. (2018). A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis. \u003cem\u003eBiochemical and Biophysical Research Communications\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eChapman, K. M., Marchi-Werle, L., Hunt, T. E., Heng-Moss, T. M., \u0026amp; Louis, J. (2018). Abscisic and Jasmonic Acids Contribute to Soybean Tolerance to the Soybean Aphid (Aphis glycines Matsumura). \u003cem\u003eScientific Reports\u003c\/em\u003e, \u003cem\u003e8\u003c\/em\u003e(1), 15148.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SD-008\/SN-009","offers":[{"title":"50 Preps","offer_id":16745411575919,"sku":"Cat #: SD-008\/SN-009","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SD-008_SN-009_1024x1024_f568e808-699e-4437-8fcc-9fea60fff568.png?v=1540414577"},{"product_id":"minute™-detergent-free-plant-protein-extraction-kit","title":"Minute™ Detergent-Free Plant Protein Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/10-SN-010_v2.pdf?v=1613417689\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_36f11319-1faa-49d9-8da1-a8840c254786.pdf?8476546550085731236\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMinute™ Detergent-Free Plant Protein Extraction Kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from plant tissues. The protein extraction buffer does not contain any detergent or organic solvent.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe extraction volume can be as small as 50 μl and as large as 500 μl using the protein extraction filter cartridges. Detergent-free total proteins can be extracted from very small plant samples (leaves, seeds, soft stems, etc.) in less than 8 minutes with a high yield (1-6 mg\/ml).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Figure_010_large.jpg?v=1550163975\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDF-Lysis Buffer (with 5g beads)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: SN-010","offers":[{"title":"50 Preps","offer_id":16745442017391,"sku":"Cat #: SN-010","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SN-010_1024x1024_10ad9d47-bc0b-4797-a645-d2399834c39f.png?v=1540414730"},{"product_id":"minute-plasma-membrane-protein-isolation-kit-for-plants","title":"Minute™ Plant Plasma Membrane\/Protein Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5p-SM-005p_v4.pdf?v=1613417660\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_47c0cabc-5d71-4cb6-ad4c-c17eaf1cf725.pdf?18003124456286137432\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning. These relatively effective methods require ultracentrifugation and a large amount of starting material. The procedures are usually tedious and time-consuming. To overcome the shortcomings, we have developed this PM isolation kit. Plant tissues are first sensitized by buffer A, homogenized and then passed through a specialized filter cartridge that allows homogenates to pass through with a zigzag path. The cell membranes are ruptured into a range of predefined sizes during the process. Native plasma membranes are separated from a mixture of unruptured cells, nuclei, cytosol and organelles by subsequent differential centrifugation and density centrifugation without using ultracentrifugation. Due to the use of the same amount of starting material, defined centrifugal force and predefined duration in every experiment, the result is much more consistent with a high degree of PM protein enrichment. The procedure can be completed in about 1 hour.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SM-005-P","offers":[{"title":"50 Preps","offer_id":16745966567535,"sku":"Cat #: SM-005-P","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SM-005-P_1024x1024_6b89c76f-4411-40f9-8512-6bd5bf1500c2.png?v=1540418227"},{"product_id":"minute-plant-microsomal-membrane-extraction-kit","title":"Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/18-MM-018_v4_087e1b44-c47e-46a9-be79-3d2c940e49e2.pdf?v=1616182195\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_fe90005d-d8a9-49dc-8acc-c5ac6e5a425c.pdf?15250503227495695386\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eIsolation of microsomal membranes from plant tissues is a common procedure. The microsomal fraction of plant cell lysate is the focus of interest in many plant research projects and is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes, and other components of a membrane system. Traditional methods for microsomal fraction isolation involve differential pelleting, where centrifugation steps are required for membrane fractions. Microsomal fraction isolation usually requires a large amount of starting material and has tedious ultracentrifugation steps. MM-018 offers a simple, fast, and user-friendly approach for microsomal membrane extraction with a small amount of starting material. During the procedures, water-soluble cytosolic proteins are removed, and water-insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a tabletop microcentrifuge. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg\/sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MM-018_large.jpg?v=1540560592\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0 ml Collection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (4)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eKim, S., Choi, Y., Kwon, C., \u0026amp; Yun, H. S. (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721\/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. \u003cem\u003eJournal of integrative plant biology\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" tabindex=\"0\"\u003eYang, Z., Yang, J., Wang, Y., Wang, F., Mao, W., He, Q., ... \u0026amp; Mao, C. (2020). PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. \u003ci\u003eThe Plant Cel\u003c\/i\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003eYu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., \u0026amp; Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. \u003ci data-mce-fragment=\"1\"\u003eMolecular Plant\u003c\/i\u003e, \u003ci data-mce-fragment=\"1\"\u003e13\u003c\/i\u003e(11), 1556-1569.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003eKato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., \u0026amp; Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. \u003cem\u003eCrop Science\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003eHou, L. Y., Lehmann, M., \u0026amp; Geigenberger, P. (2021). Thioredoxins o1 and h2 Show Different Subcellular Localizations and Redox-Active Functions, but Cooperatively Affect NADPH Redox Poise and Photosynthetic Performance in Fluctuating Light.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003eChiu, C. Y., Tsai, C. D., Wang, J. Y., Tsai, M. H., Kanno, S., Lung, H. F., \u0026amp; Liu, T. Y. (2024). Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis. \u003ci\u003ebioRxiv\u003c\/i\u003e, 2024-06.\u003c\/span\u003e\u003cb style=\"mso-bidi-font-weight: normal;\"\u003e\u003cspan style=\"font-size: 12.0pt; mso-bidi-font-size: 11.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman';\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: MM-018","offers":[{"title":"50 Preps","offer_id":16746183065711,"sku":"Cat #: MM-018","price":699.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/MM-018_1024x1024_a5fcdb1a-c37a-4150-b786-f5fd2bcef696.png?v=1540419540"},{"product_id":"minute-chloroplast-isolation-kit","title":"Minute™ Chloroplast Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/11-CP-11_v6.pdf?v=1613417694\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_e725b4e9-433b-4de1-a9b1-c8d0b59bbce2.pdf?14120686575185508492\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eInvent Biotechnologies Minute\u003csup\u003eTM \u003c\/sup\u003echloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 50-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 10\u003csup\u003e6\u003c\/sup\u003e to 1 X 10\u003csup\u003e7\u003c\/sup\u003e intact chloroplasts (\u0026gt;90% intact) from fresh plant leaves.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/CP-011_large.jpg?v=1540560637\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 358px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eBuffer A (with 5 grams dissociation beads)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cbr\u003e\n\u003c\/div\u003e\nBuffer B\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: CP-011","offers":[{"title":"50 Preps","offer_id":16746252206191,"sku":"Cat #: CP-011","price":394.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/CP-011_1024x1024_628e6114-598c-40a1-a562-dc63bed29332.png?v=1540419828"},{"product_id":"minute™-plant-cytosolic-and-nuclear-protein-isolation-kit","title":"Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/45-PF-045_V2.pdf?v=1613417908\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MSDS-6_92b0575b-fc12-4e0b-9347-c78ec3c2a3dc.pdf?798\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #0000ff;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Comparison_of_Plant_Nuclei_Isolation_Kits.pdf?801\" style=\"color: #0000ff;\" target=\"_blank\"\u003eCompare with leading Plant Nuclei Isolation Kit\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is designed for rapid fractionation of fresh\/frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100-200 mg starting material.  By using a specialized filter cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol, nuclei, chloroplasts and organelles) can be obtained in about 1 hour. The subcellular fractions can be used in numerous applications that include but not limited to protein trafficking, nuclear protein extraction and nucleic acid purification\/sequencing. The highly enriched nuclei can be used for proteomics, flow cytometry analysis, DNA sequencing, and protein-protein\/protein-DNA interaction studies. The subcellular fractionation protocol includes grinding tissue to release plant cells followed by disruption of the cell membrane by rapidly passing through the filter. Different subcellular fraction is obtained by differential centrifugation using only a table top centrifuge. This kit has been tested for use with many species such as Arabidopsis, Spinach, Peas, and Rape.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/N-Tube-F_large.jpg?v=1565370907\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Nucei-F_copy_large.jpg?v=1565370914\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Plant-Nu-WB-F_large.jpg?v=1565370919\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"527\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePestle for 1.5 ml Eppendorf tubes\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Power\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 gram\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003ch5\u003e\u003cstrong\u003eReferences (2)\u003c\/strong\u003e\u003c\/h5\u003e\n\u003cp\u003e\u003cstrong\u003e1. \u003c\/strong\u003eSon, S., Moon, S. J., Kim, H., Lee, K. S., \u0026amp; Park, S. R. (2021). Identification of a novel NPR1 homolog gene, OsNH5N16, which contributes to broad-spectrum resistance in rice. \u003cem\u003eBiochemical and Biophysical Research Communications\u003c\/em\u003e, \u003cem\u003e549\u003c\/em\u003e, 200-206.\u003c\/p\u003e\n\u003cp\u003e2. Im, J. H., Son, S., Ko, J. H., Kim, K. H., An, C. S., \u0026amp; Han, K. H. (2021). Nuclear Translocation of Soybean MPK6, GmMPK6, Is Mediated by Hydrogen Peroxide in Salt Stress. \u003cem\u003ePlants\u003c\/em\u003e, \u003cem\u003e10\u003c\/em\u003e(12), 2611.\u003c\/p\u003e","brand":"Cat #: PF-045","offers":[{"title":"20 preps","offer_id":29482883907695,"sku":"Cat #: PF-045","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Minute_Plant_Cytosolic_and_Nuclear_Protein_Isolation_Kit_Need_150_mg_Raw_Material_20_preps.png?v=1751897706"},{"product_id":"minute™-rubisco-depletion-kit","title":"Minute™ Rubisco Depletion Kit (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/46-RD-046_v3.pdf?v=1613417916\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MSDS-6_92b0575b-fc12-4e0b-9347-c78ec3c2a3dc.pdf?798\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePlants perceive signals from the environment through leaves. One of the major goals of the scientific community is to dissect the components of signaling cascades by analyzing the leaf proteome. However, the major bottleneck is presence of a high-abundant protein —Rubisco, which accounts for majority of leaf protein ranging from 40-80%. High level rubisco masks the detection and identification of low abundant proteins by LC-MS\/MS-based method. A specific IgY conjugated resin is available commercially for depletion of rubisco from extracted total protein but only small amount of protein can be handled and it is very costly. Other methods have been described in the literatures but are usually tedious and time consuming. The Minute\u003csup\u003eTM\u003c\/sup\u003e Rubisco Depletion Kit is designed to overcome these disadvantages by using a specialized filter cartridge coupled with proprietary rubisco depletion buffer. The same, if not better, results can be obtained with a much easier and straighter forward protocol in about 1h. The rubisco-depleted total protein can be used for many downstream applications such as SDS-PAGE, 2DE and LC MS\/MS analysis.\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Rubisco-data-F_large.jpg?v=1565378144\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestle for 1.5 ml microfuge tube\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: RD-046","offers":[{"title":"20","offer_id":29483149721711,"sku":"Cat #: RD-046","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Minute_Rubisco_Depletion_Kit_20_preps.png?v=1751898392"},{"product_id":"minute™-plant-er-enrichment-kit","title":"Minute™ Plant ER Enrichment Kit (20 preps)","description":"\u003ch2 data-mce-fragment=\"1\"\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/48-PR-048_v3_f2413375-2012-473c-ba95-4d6a60c933d1.pdf?v=1753371739\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8.pdf?v=1603134855\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIsolation and enrichment of plant endoplasmic reticulum (ER) are essential for elucidating ER-related cellular functions. The traditional method for ER isolation is tedious and time-consuming.  In animal cells, ER is closely associated with microtubules, and Golgi apparatus are clustered at microtubule organizing center. The ER is associated with actin microfilaments in plant cells, and no microtubule organizing center is found near the nuclei. Due to the structural characteristics of plan ER, it is much more challenging to isolate\/enrich plant ER than animal samples. Minute™ plant ER enrichment kit was specially designed for plants. One to three-fold enrichment of ER fraction can be obtained from plant leave samples in about 1 hour. The enriched ER is essentially free from Golgi apparatus contamination. The enriched ER fraction can be used in plant protein trafficking studies.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Plant-ER-F-1_cc1b8915-0ea3-4b66-b738-6539d408c28e_480x480.jpg?v=1603132314\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003eA. Ponceau S Stained Blot; B. Western blot with Anti-ER marker antibody (BIP2, Agrisera, Vannas, Sweden. AS 09481). Lanes 1\u0026amp;2: B. napus; Lanes 3\u0026amp;4: N. tabacum; Lanes 5\u0026amp;6: A. thaliana. Lanes 1, 3 \u0026amp; 5: Total Protein; Lanes 2, 4 \u0026amp; 6: ER fractions.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e 20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003eBuffer B\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e1.2 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003eBuffer C\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003ePlastic Rods\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003eFilter Cartridge\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 70.2809%;\"\u003e2 ml Collection Tibe\u003c\/td\u003e\n\u003ctd style=\"width: 23.7191%;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"text-align: center;\" data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: PR-048","offers":[{"title":"20 preps","offer_id":32154277118063,"sku":"Cat #: PR-048","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PR-048.png?v=1779381519"},{"product_id":"minute™-plant-golgi-apparatus-enrichment-kit-20-preps","title":"Minute™ Plant Golgi Apparatus Enrichment Kit (20 preps)","description":"\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/49-PG-049_v2_09d8219c-2a39-40ca-9666-21900f5632d4.pdf?v=1630504689\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca data-mce-fragment=\"1\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8.pdf?v=1603134855\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe plant Golgi apparatus plays an important role in the biosynthesis of cellular structural components and protein trafficking. However proteomic characterization of the Golgi has been hindered by limited methodologies for obtaining enriched\/purified Golgi fraction. The traditional method for isolating Golgi apparatus depends upon the use of density centrifugation, which is tedious and time-consuming. A large amount of starting material is usually required. Plant Golgi stacks are notoriously labile and loss of stack integrity can occur if harsh homogenization is used. This means that assessment of purity by morphological means such as electron microscopy is difficult. This kit is designed to overcome certain disadvantages of traditional methods by employing spin column-based homogenization, differential centrifugation, and selective precipitation for the enrichment of the Golgi. The protocol is simple and straightforward and requires only a milligram amount of starting material. Significant enrichment of the Golgi fraction (2-3 folds) can be obtained.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eEnrichment of Golgi Apparatus from Plant Leaves\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Plant_Golgi-1_data_480x480.jpg?v=1603135478\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eA.\u003c\/strong\u003e Ponceau S stained blot. \u003cstrong\u003eB\u003c\/strong\u003e. Western blot with ER (BIP2), Golgi (Arf1) and histone 3 (H3) marker antibodies. The antibodies were purchased from Agrisera (Vannas, Sweden). Lanes 1\u0026amp;2: total protein from A. thaliana and N. tabacum; Lanes 3\u0026amp;4: enriched Golgi fractions from A. thaliana and N. tabacum, respectively.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.0 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4 ml\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer D\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: PG-049","offers":[{"title":"20 preps","offer_id":32154373521519,"sku":"Cat #: PG-049","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PG-049.png?v=1779381415"},{"product_id":"minute™-plant-endosome-enrichment-kit-20-preps","title":"Minute™ Plant Endosome Enrichment Kit (20 preps)","description":"\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/50-PE-050_v2.pdf?v=1613417945\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca data-mce-fragment=\"1\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8.pdf?v=1603134855\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAn understanding of endomembrane processes and their biological implication is important for understanding plant growth and development. Endosomes are a heterogeneous collection of organelles in the sorting and delivery of internalized material from cell surface and the transport of materials from Golgi to lysosome or vacuole. In plant cells, two clearly defined endosomal compartments have been identified: the trans-Golgi network (early endosome equivalent) and the multivesicular body (late endosome equivalent). Traditionally, methods for isolation and purification of these two compartments are tedious and time-consuming involving sucrose gradient centrifugation. In some cases, sucrose gradient isolation is coupled with immunoaffinity purification and larger amount of starting materials are usually required (10-50 g). We have developed this kit using a spin-column-based format coupling with selective precipitation for enrichment of endosomal compartment. This approach is easy and rapid and only small amount of starting material is required. \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Planr_Endosome-Final_data_480x480.jpg?v=1603137157\"\u003e\u003c\/p\u003e\n\u003cp\u003eDetection of Enriched Endosomes from N. benthmiana by  Western blotting. The plant leaf that carries GFP-tagged  ara6 (early endosome marker) was processed as described in the protocol and probed with anti-ara6 and anti-histone  antibodies. Lane 1, Total tissue lysate; lane 2, Supernatant  from step 4 of the protocol; lane 3, Enriched endosome fraction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e15 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: PE-050","offers":[{"title":"20 preps","offer_id":32154463273071,"sku":"Cat #: PE-050","price":419.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PE-050.png?v=1779381361"},{"product_id":"minute™-plant-lipid-raft-isolation-kit-20-preps","title":"Minute™ Plant Lipid Raft Isolation Kit (20 preps)","description":"\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/51-PL-051_v2.pdf?v=1613417951\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8.pdf?v=1603134855\" data-mce-fragment=\"1\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThere are substantial evidences that support the existence of detergent-resistant membrane (DRM) subdomains in both animal and plant membrane systems, which is also referred to as lipid rafts. Lipid rafts are shown to have increased sphingolipid to protein ratio and higher cholesterol concentration. The lipid rafts are believed to play an important role in signal transduction and protein trafficking in eukaryotic cells. Traditional method for lipid raft isolation involves cold non-ionic detergent extraction followed by sucrose gradient ultracentrifugation. The protocol requires large amount of starting material (hundred grams amount) and specialized equipment in addition to lengthy protocol time. We have developed a rapid method for isolation of lipid rafts from plant tissues using only milligram amount of starting material and the protocol can be completed in about 1h without using ultracentrifugation.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Plant_LR-Final_data_480x480.jpg?v=1603138401\" alt=\"\" data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp\u003eWestern Blotting of Isolated Lipid Rafts. A. Ponceau red stained blot membrane. B. Western Blot. Samples: Leaves of Spinacia oleracea (1,2,3) and Blassica rapa (Lanes 4,5,6). Lanes 1 \u0026amp; 4, total tissue lysates. Lanes 2,3 \u0026amp; 4,5; isolated Lipid rafts.  Rabbit anti H+ATPase (plasma membrane marker), and cFBPase (cytosolic marker) were purchased from Agrisera (Vannas, Sweden).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.2 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer D\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003e\n\u003cstrong\u003eReference\u003c\/strong\u003e (1)\u003c\/h5\u003e\n\u003cp\u003e1. Zhou, N., Li, X., Zheng, Z., Liu, J., Downie, J. A., \u0026amp; Xie, F. (2024). RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip. \u003ci\u003eNature Communications\u003c\/i\u003e, \u003ci\u003e15\u003c\/i\u003e(1), 1-16.\u003c\/p\u003e","brand":"Cat #: PL-051","offers":[{"title":"20 preps","offer_id":32154529792111,"sku":"Cat #: PL-051","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PL-051.png?v=1779381461"},{"product_id":"minute™-anti-clumping-nuclei-storage-buffer-10-ml","title":"Minute™ Anti-Clumping Nuclei Storage Buffer (10 ml)","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-014_v1.pdf?v=1633973539\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8_edbbb1d9-57b5-4450-b6fd-bc6ee6e95ace.pdf?v=1633969533\"\u003eMSDS\u003c\/a\u003e                                        \u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe traditional method for nuclei isolation from animal cells\/tissues depends on the use of non-ionic detergent to lyse the plasma membrane and release nuclei which can be subsequently isolated by low-speed centrifugation and further purified by density gradient. One major problem associated with the traditional method is the clumping of nuclei after isolation, which, in some applications such as DNA and RNA extraction, may not be a problem. However, in other applications such as single nucleus sequencing or proteomics, clumping of isolated nuclei represents a major problem. The anti-clumping nuclei storage buffer is designed to address this issue. Nuclei resuspended in this buffer show significantly reduced clumping as compared to those resuspended in PBS with 5% BSA. The nucleus suspension can be stored at 4\u003csup data-mce-fragment=\"1\"\u003eo\u003c\/sup\u003eC for days without significant aggregation and change in morphology. The nuclei can also be frozen in the buffer at -80\u003csup data-mce-fragment=\"1\"\u003eo\u003c\/sup\u003eC or in dry ice for transportation or long-term storage.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor spin column-based single nuclei isolation, \u003ca href=\"https:\/\/inventbiotech.com\/collections\/single-nuclei-isolation\"\u003eclick here\u003c\/a\u003e for the list of available products.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Anti-aggreg-24hS_data_480x480.jpg?v=1633720529\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComparison of the Storage Buffer with 5% BSA in PBS for Anti-Clumping Efficacy.\u003c\/strong\u003e Nuclei of frozen mouse spleen and lung tissues were isolated by Nucleus Isolation Kit for Tissues\/cells (Cat# SN-047, Invent Biotechnologies, Plymouth MN). The isolated nuclei pellets were resuspended in 0.4 ml nuclei storage buffer or 0.4 ml 5% BSA in PBS and incubated at 4\u003csup\u003eo\u003c\/sup\u003eC overnight.  The nuclei were resuspended by vortexing briefly and stained with trypan blue to compare the nuclear morphologies.\u003c\/p\u003e","brand":"Cat #: WA-014","offers":[{"title":"Default Title","offer_id":40618659709039,"sku":"Cat #: WA-014","price":373.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-014.png?v=1779381701"},{"product_id":"minute™-chloroplast-isolation-kit-for-mucilaginous-plants-20-preps","title":"Minute™ Chloroplast Isolation kit for Mucilaginous Plants (20 preps)","description":"\u003ch2 data-mce-fragment=\"1\"\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/53-CF-053_v1.pdf?v=1662053029\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8_b65d4b78-b81b-4293-b5e7-d633118b4953.pdf?v=1662053250\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eMany crop species across the plant family contain high polysaccharide and polyphenols. The hydrocolloid mucilage is a complex mucopolysaccharide typically showing high viscosity when released in solution. Homogenate of high mucopolysaccharide plant samples usually imparts a slimy consistency that is most unwanted contaminants that interfere with cell fractionation and organelle purification, such as chloroplast and nuclear isolation. This kit provides a simple, rapid, and efficient method for isolating chloroplasts from viscous plant homogenate. The protocol takes about 30 min.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Sticky_chloroplasts-F1_copy_4f7f71b6-8726-476e-b449-c55eab70a5d4_480x480.png?v=1662051826\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 1. Chloroplasts isolated from leaves of indicated plant species.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eStrawberry                                                     Plum \u003c\/strong\u003e    \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Chloroplasts-F2_480x480.png?v=1662051842\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" style=\"text-align: center;\"\u003e\u003cstrong\u003eSweet Potato                                         Water Spinach\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 2.  Morphologies of chloroplasts isolated from leaves of indicated plants under light microscopy.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 73.3257%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"width: 23.555%;\"\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 73.3257%;\"\u003eBuffer B\u003c\/td\u003e\n\u003ctd style=\"width: 23.555%;\"\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 73.3257%;\"\u003eMicro Puncher\u003c\/td\u003e\n\u003ctd style=\"width: 23.555%;\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: CF-053","offers":[{"title":"Default Title","offer_id":40926972215407,"sku":"Cat #: CF-053","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/CF-053.png?v=1779381179"},{"product_id":"minute™-single-nuclei-isolation-kit-for-plant-tissues-20-preps","title":"Minute™ Single Nuclei Isolation Kit for Plant Tissues (20 preps)","description":"\u003ch2 data-mce-fragment=\"1\"\u003e \u003cstrong data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/54-PS-054_v1_43338e60-92fd-418f-a0e4-f2d58de02314.pdf?v=1667227611\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8_b65d4b78-b81b-4293-b5e7-d633118b4953.pdf?v=1662053250\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSingle nuclei have become the ideal materials for next-generation genomics and proteomics. However, isolating high-quality single nuclei from plant tissues remains challenging. Most lab-developed methods are limited to specific tissue or species, and no standard protocol and buffer system exist to handle all tissue types from different species. Significant optimization may be needed for different samples. One major drawback of traditional methods is the clumping or aggregation of isolated nuclei, making them difficult for applications such as single nucleus RNA-seq and ATAC analysis. This kit is designed to rapidly isolate well-separated single nuclei from tissues of model and widely-studied plants with minimal clumping using a specially designed spin column and proprietary buffers.  The protocol takes about 30 minutes and requires only 150-200mg of the starting material.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Single-Nuclei-F1_480x480.png?v=1662056904\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003eFigure 1. Single nuclei isolated from plant leaves.\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003eTrypan blue staining (magnification: X 100)\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Single_DNA-F2_2_480x480.jpg?v=1662056917\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003eFigure 2. Crude genomic DNAs extracted from isolated nuclei\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e1. A. Thaliana, 2. Soybean, 3. S. Viridis, 4. Maize\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003e Buffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003eBuffer C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003e2.0 ml collection tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 71.254%;\"\u003e\n\u003cp\u003ePlastic Rod\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.746%;\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cat #: PS-054","offers":[{"title":"Default Title","offer_id":40927031459951,"sku":"Cat #: PS-054","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PS-054.png?v=1779381563"},{"product_id":"minute™-thylakoid-enrichment-and-chloroplast-fractionation-kit-20-preps","title":"Minute™ Thylakoid Enrichment and Chloroplast Fractionation Kit (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/55-CF-055_v1_ff6ac608-d7dc-4c93-9a46-3a6785372842.pdf?v=1663956721\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8_a05d406d-7f5e-457c-bc6c-50ad2b45d389.pdf?v=1663869179\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eA chloroplast, delimited by a two-membrane envelope, is a type of plastid that serves as the site of photosynthesis. The photosynthetic function is based on developing an operational extensive internal membrane network, called the thylakoids, and on enzymatic processes in the chloroplast matrix, called the stroma. Thylakoid membrane is structurally different from the chloroplast envelope, and their biogenesis depends on biosynthetic and transporting activities specific to the chloroplast envelope. This kit is designed to fractionate chloroplasts into three parts \u003cstrong\u003e(thylakoid membrane, envelope membrane, and stroma)\u003c\/strong\u003e using a specialized filter cartridge and differential centrifugation (without ultracentrifugation). Isolated thylakoids are in native forms and can be used for functional studies and the studies of subcellular localization of proteins using Western blotting\/Mass spectrometry. The buffers in the kit are detergent and EDTA-free. The protocol can be completed in about one hour.\u003c\/p\u003e\n\u003cp\u003e\u003cimg\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Three_layers_copy-F1_480x480.png?v=1663864849\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003eT: Left, S: Middle, E: Right\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e Fractionation of chloroplasts from spinach leaf into three fractions: Thylakoid (T), Stroma (S), and Envelope membranes (E).\u003c\/p\u003e\n\u003cp\u003e                                           \u003cstrong\u003e A. thaliana\u003c\/strong\u003e                   \u003cstrong\u003eS. oleracea\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e                                           1    2    3    4                  \u003cspan\u003e1    2    3    4\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"248\" width=\"292\" style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Chloroplast-Gel-F-2_copy_480x480.png?v=1663865050\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e SDS-PAGE (Upper) and Western blot (Lower) of fractionated chloroplasts.   Lane 1. Total tissue lysate, 2. Thylakoid, 3. Stroma and 4. Envelope membranes. Thylakoid marker antibody was purchased from Agrisera (AS05084).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 55.9742%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"width: 40.0258%; text-align: center;\"\u003e25 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 55.9742%;\"\u003eBuffer B\u003c\/td\u003e\n\u003ctd style=\"width: 40.0258%; text-align: center;\"\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 55.9742%;\"\u003eFilter Cartridge \/ 2.0 ml Collection Tube\u003c\/td\u003e\n\u003ctd style=\"width: 40.0258%; text-align: center;\"\u003e40 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 55.9742%;\"\u003ePestles for 1.5 ml Eppendorf Microtubes \u003c\/td\u003e\n\u003ctd style=\"width: 40.0258%; text-align: center;\"\u003e2 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 55.9742%;\"\u003eProtein Extraction Powder\u003c\/td\u003e\n\u003ctd style=\"width: 40.0258%; text-align: center;\"\u003e5g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: CF-055","offers":[{"title":"Default Title","offer_id":40975558115439,"sku":"Cat #: CF-055","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/CF-055.png?v=1779381226"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/collections\/Logo_maker_project-1_4.png?v=1763407907","url":"https:\/\/inventbiotech.com\/collections\/plant-specific-products.oembed","provider":"Invent Biotechnologies Inc.","version":"1.0","type":"link"}