Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plant, bacteria, yeast and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.

Kit includes:

1. Kalabus, JL. et al. (2012). MicroRNAs differentially regulate carbonyl reductase 1 (CBR1) gene expression dependent on the allele status of common polymorphic variant rs9024. PLOS ONE. 7(11):1-9.
2. Lombrafia AQ. et al (2014). Interindividual variability in the cardiac expression of anthracycline reductases in donors with and without down syndrome. Pharm Res. 31:1644-1655.
3. Hou, N., et al. (2014). Vitamin A deficiency impairs spatial learning and memory: The mechanism of abnormal CBP-dependent histone acetylation regulated by retinoic acid receptor alpha. Mol Neurobiol. DOI 10.1007/s12035-014-8741-6.
4. You, Y., et al. (2014). Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3. Cell Death and Disease. (2014)5, e1493; doi: 10.1038/cddis. 2014.443.
5. Tanaka M et al. (2016). Ex vivo effects of naphthoquinones allergen-sensitized mononuclear cells in mice. International Journal of Immunopathology and Pharmacology. DOI: 10.1177/0394632016632221
6. Liu W. et al. (2016) Electroacupunctre improves motor impairment via inhibition of microglia-mediated neuroinflammation in the sensorimotor cortex after ischemic stroke. Life Sciences. doi:10.1016/j.lfs.2016.01.045
7. Xu, Yang, et al. (2016), RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF." Scientific Reports 6:29362.
8. Shi, Le, et al. (2016). "Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis." Tumor Biology: 1-13.9
9. Huang, C. W., Feng, W., Peh, M. T., Peh, K., Dymock, B. W., & Moore, P. K. (2016). A novel slow-releasing hydrogen sulfide donor, FW1256, exerts anti-inflammatory effects in mouse macrophages and in vivo. Pharmacological Research.
10. Matsuoka, Y., Nakayama, H., Yoshida, R., Hirosue, A., Nagata, M., Tanaka, T., ... & Shinriki, S. (2016). IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. British Journal of Cancer.
11. Meng, C., Cui, X., Qi, S., Zhang, J., Kang, J., & Zhou, H. (2016). Lung inflation with hydrogen sulfide during the warm ischemia phase ameliorates injury in rat donor lungs via metabolic inhibition after cardiac death. Surgery.
12. Liu, Y., Peng, J., Sun, T., Li, N., Zhang, L., Ren, J., ... & Ding, Y. (2017). Epithelial EZH2 serves as an epigenetic determinant in experimental colitis by inhibiting TNFα-mediated inflammation and apoptosis. Proceedings of the National Academy of Sciences, 201700909.
13. Chen, Y., Hu, X., Guo, C., Yu, Y., & Lu, H. (2017). Epe1 contributes to activation of AMPK by promoting phosphorylation of AMPK alpha subunit, Ssp2. Scientific Reports, 7.
14. Hu, H., Zhu, W., Qin, J., Chen, M., Gong, L., Li, L., ... & Ye, D. (2016). Acetylation of PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology
15. Hou, Y., Wang, Y., He, Q., Li, L., Xie, H., Zhao, Y., & Zhao, J. (2017). Nrf2 Inhibits NLRP3 Inflammasome Activation Through Regulating Trx1/TXNIP Complex in Cerebral Ischemia Reperfusion Injury. Behavioural Brain Research.
16. Ueda, K., Masuda, A., Fukuda, M., Tanaka, S., Hosokawa, M., & Iwakawa, S. (2017). Monophosphorylation by deoxycytidine kinase affects apparent cellular uptake of decitabine in HCT116 colon cancer cells. Drug Metabolism and Pharmacokinetics.
17. Inoue, T., Ninuma, S., Hayashi, M., Okuda, A., Asaka, T., & Maejima, H. (2017). Effects of long-term exercise and low-level inhibition of GABAergic synapses on motor control and the expression of BDNF in the motor related cortex. Neurological Research, 1-8.
18. Yang, Z., Zhu, M. L., Li, D. H., Zeng, R., & Han, B. N. (2017). N-Me- trichodermamide B isolated from Penicillium janthinellum, with antioxidant properties through Nrf2-mediated signaling pathway. Bioorganic & Medicinal Chemistry.
19. Ding, C. H., Deng, L. F., Chen, F., Ding, K., Chen, W. S., Xie, W. F., & Zhang, X. (2017). p. Q511L mutation of HNF1α in hepatocellular carcinoma suppresses the transcriptional activity and the anti-tumor effect of HNF1α. Biochemical and Biophysical Research Communications.
20. Zhang, F., Zhao, L., Wang, S., Yang, J., Lu, G., Luo, N., ... & Wei, W. (2017). Construction of a Biomimetic Magnetosome and Its Application as a SiRNA Carrier for High‐Performance Anticancer Therapy. Advanced Functional Materials.
21. Kawaguchi, R., Hirata, N., Tokinaga, Y., Hayashi, S., Inoue, S., Watanabe, N., & Yamakage, M. (2017). Nitrite administration improves sepsis-induced myocardial and mitochondrial dysfunction by modulating stress signal responses. Journal of Anesthesia, 31(6), 885-894.
22. Liu, L., Wu, W., Li, J., Jiao, W. H., Liu, L. Y., Tang, J., ... & Lin, H. W. (2018). Two sesquiterpene aminoquinones protect against oxidative injury in HaCaT keratinocytes via activation of AMPKα/ERK-Nrf2/ARE/HO-1 signaling. Biomedicine & Pharmacotherapy, 100, 417-425.
23. Mo, J., Xie, Q., Wei, W., & Zhao, J. (2018). Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona. Nature communications, 9(1), 2480.
24. Liu, Q., Zhao, Y., Zheng, L., Zhu, X., Cui, L., & Cao, Y. (2018). The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria. Infection and immunity, IAI-00929.
25. Li, J., Liu, Q., Liu, Z., Xia, Q., Zhang, Z., Zhang, R., ... & Chen, X. (2018). KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc. Journal of Experimental & Clinical Cancer Research, 37(1), 194.
26. Kang, P. C., Leng, K. M., Liu, Y. P., Liu, Y., Xu, Y., Qin, W., ... & Cui, Y. F. (2018). miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma. Cellular Physiology and Biochemistry, 49(5), 1933-1942.
27. Yin, J., Wang, Y., Chang, J., Li, B., Zhang, J., Liu, Y., ... & Zeng, X. (2018). Apelin inhibited epithelial− mesenchymal transition of podocytes in diabetic mice through downregulating immunoproteasome subunits β5i. Cell Death & Disease, 9(10), 1031.
28. Sakurai, H., Morishima, Y., Ishii, Y., Yoshida, K., Nakajima, M., Tsunoda, Y., ... & Yamamoto, M. (2018). Sulforaphane ameliorates steroid insensitivity through an Nrf2-dependent pathway in cigarette smoke-exposed asthmatic mice. Free Radical Biology and Medicine.
29. Hou, J., Shi, J., Chen, L., Lv, Z., Chen, X., Cao, H., ... & Han, X. (2018). M2 macrophages promote myofibroblast differentiation of LR-MSCs and are associated with pulmonary fibrogenesis. Cell Communication and Signaling, 16(1), 89.
30. Zhang, C., Wu, W., Zhu, H., Yu, X., Zhang, Y., Ye, X., ... & Guan, J. (2018). Knockdown of long non-coding RNA CCDC144NL-AS1 attenuates migration and invasion phenotypes in endometrial stromal cells from endometriosis. Biology of Reproduction.
31. Wei S, Yang D, Yang J, Zhang X, Zhang J, FuJ, Zhou G, Liu H, Lian Z, Han H, (2018) Overexpression of Toll-like receptor 4enhances LPS-induced inflammatory response and inhibits Salmonella Typhimurium growth in ovine macrophages, European Journal of Cell Biology. https://doi.org/10.1016/j.ejcb.2018.11.004
32. Otani, T., Matsuda, M., Mizokami, A., Kitagawa, N., Takeuchi, H., Jimi, E., ... & Hirata, M. (2018). Osteocalcin triggers Fas/FasL-mediated necroptosis in adipocytes via activation of p300. Cell Death & Disease, 9(12), 1194.
33. Qian, Q., Zhang, J., He, F. P., Bao, W. Y., Zheng, T. T., Zhou, D. M., ... & Sun, B. G. (2018). Down-regulated expression of microRNA-338-5p contributes to neuropathology in Alzheimer’s disease. The FASEB Journal, fj-201801846R.
34. Li, R., Zhang, Y., Li, Y., Xue, L., & Wang, W. (2018). Hyperbaric oxygen treatment promotes antioxidative effects on rats with acute carbon monoxide poisoning via NF-E2-related factor 2 activation. Int J Clin Exp Med, 11(12), 13274-13285.
35. Bao, W., Liu, X., Lv, Y., Lu, G. H., Li, F., Zhang, F., ... & Li, Y. (2019). Nanolongan with Multiple On-Demand Conversions for Ferroptosis-Apoptosis Combined Anticancer Therapy. ACS Nano.
36. Zhao, W., Jia, L., kuai, X., Tang, Q., Huang, X., Yang, T., ... & Feng, Z. The role and molecular mechanism of Trop2 induced epithelial‐mesenchymal transition through mediated β‐catenin in gastric cancer. Cancer Medicine.
37. Zhou, Z., Jiang, H., Tu, K., Yu, W., Zhang, J., Hu, Z., ... & Wang, A. (2019). ANKHD1 is required for SMYD3 to promote tumor metastasis in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research, 38(1), 18.
38. Li, P. C., Li, K., Wang, J., Zhao, C. Z., Zhao, S. Z., Hou, L., ... & Wang, X. J. (2019). The AAA-ATPase MIDASIN 1 Functions in Ribosome Biogenesis and Is Essential for Embryo and Root Development. Plant physiology, pp-01225.
39. Kong, P., Yu, Y., Wang, L., Dou, Y. Q., Zhang, X. H., Cui, Y., ... & Cui, W. (2019). circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells. Nucleic Research.

40. Fu, J., Li, G., Wu, X., & Zang, B. Sodium Butyrate Ameliorates Intestinal Injury and Improves Survival in a Rat Model of Cecal Ligation and Puncture-Induced Sepsis. Inflammation, 1-11.

41. Zhu, Y., Shao, Y., Qu, X., Guo, J., Yang, J., Zhou, Z., & Wang, S. (2019). Sodium ferulate protects against influenza virus infection by activation of the TLR7/9-MyD88-IRF7 signaling pathway and inhibition of the NF-κB signaling pathway. Biochemical and Biophysical Research Communications.
42. Sanada, T. J., Sakao, S., Naito, A., Ishibashi-Ueda, H., Suga, M., Shoji, H., ... & Ishida, K. (2019). Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. PloS one, 14(3), e0214654.
43. Lin, W., Xu, L., Pan, Q., Lin, S., Feng, L., Wang, B., ... & Wang, Y. (2019). Lgr5-overexpressing mesenchymal stem cells augment fracture healing through regulation of Wnt/ERK signaling pathways and mitochondrial dynamics. The FASEB Journal, fj-201900082RR.
44. Liu, B., Gu, Y., Pei, S., Peng, Y., Chen, J., Pham, L. V., ... & Wang, H. (2019). Interleukin-1 receptor associated kinase (IRAK)-M-mediated type 2 microglia polarization ameliorates the severity of experimental autoimmune encephalomyelitis (EAE). Journal of Autoimmunity.
45. Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F., ... & Guo, C. (2019). Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion. International journal of molecular medicine, 43(6), 2507-2515.
46. Li, M., Zhang, H., Liu, H., Tian, H., Tang, X., Bai, Y., & Wang, W. (2019). Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations. Biological Research, 52(1), 27.
47. Huang, C., Chen, Y., Dai, H., Zhang, H., Xie, M., Zhang, H., ... & Chen, Z. (2019). UBAP2L arginine methylation by PRMT1 modulates stress granule assembly. Cell Death & Differentiation, 1.
48. Kang, Y., Li, Y., Zhang, T., Chi, Y., & Liu, M. Effects of transcription factor EB on oxidative stress and apoptosis induced by high glucose in podocytes. International Journal of Molecular Medicine.
49. Ren, R. C., Wang, L. L., Zhang, L., Zhao, Y. J., Wu, J. W., Wei, Y. M., ... & Zhao, X. Y. DEK43 is a P‐type PPR Protein Responsible for the Cis‐splicing of nad4 in Maize Mitochondria. Journal of Integrative Plant Biology.
50. Zhu, W., Xu, R., Du, J., Fu, Y., Li, S., Zhang, P., ... & Jiang, H. (2019). Zoledronic acid promotes TLR-4–mediated M1 macrophage polarization in bisphosphonate-related osteonecrosis of the jaw. The FASEB Journal, 33(4), 5208-5219.
51. Ye, M., Ma, J., Liu, B., Liu, X., Ma, D., & Dong, K. Linc01105 acts as an oncogene in the development of neuroblastoma. Oncology Reports.
52. Sun, L. J., Qiao, W., Xiao, Y. J., Cui, L., Wang, X., & Ren, W. D. (2019). Naringin mitigates myocardial strain and the inflammatory response in sepsis-induced myocardial dysfunction through regulation of PI3K/AKT/NF-κB pathway. International Immunopharmacology, 75, 105782.
53. Diao, H., Cheng, N., Zhao, Y., Xu, H., Dong, H., Thamm, D. H., ... & Lin, D. (2019). Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling. BMC Veterinary Research, 15(1), 1-10.
54. Sun, L. J., Qiao, W., Xiao, Y. J., Cui, L., Wang, X., & Ren, W. D. (2019). Naringin mitigates myocardial strain and the inflammatory response in sepsis-induced myocardial dysfunction through regulation of PI3K/AKT/NF-κB pathway. International Immunopharmacology, 75, 105782.
55. Sun, L. J., Qiao, W., Xiao, Y. J., Cui, L., Wang, X., & Ren, W. D. (2019). Naringin mitigates myocardial strain and the inflammatory response in sepsis-induced myocardial dysfunction through regulation of PI3K/AKT/NF-κB pathway. International Immunopharmacology, 75, 105782.
56. Diao, H., Cheng, N., Zhao, Y., Xu, H., Dong, H., Thamm, D. H., ... & Lin, D. (2019). Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling. BMC Veterinary Research, 15(1), 1-10.
57. Tong, S. M., Wang, D. Y., Gao, B. J., Ying, S. H., & Feng, M. G. The DUF1996 and WSC domain‐containing protein Wsc1I acts as a novel sensor of multiple stress cues in Beauveria bassiana. Cellular Microbiology, e13100.Cao, H., Chen, X., Hou, J., Wang, C., Xiang, Z., Shen, Y., & Han, X. (2019).
58. The Shh/Gli signaling cascade regulates myofibroblastic activation of lung-resident mesenchymal stem cells via the modulation of Wnt10a expression during pulmonary fibrogenesis. Laboratory Investigation, 1-15.
59. Liu, L., Zhu, H., Wu, W., Shen, Y., Lin, X., Wu, Y., ... & Lin, H. W. (2019). Neoantimycin F, a Streptomyces-Derived Natural Product Induces Mitochondria-Related Apoptotic Death in Human Non-Small Cell Lung Cancer Cells. Frontiers in Pharmacology, 10.
60. Zhang, Y., Xie, H., Tang, W., Zeng, X., Lin, Y., Xu, L., ... & Yuan, D. (2019). Trichostatin A, a histone deacetylase inhibitor, alleviates eosinophilic meningitis induced by Angiostrongylus cantonensis infection in mice. Frontiers in Microbiology, 10.
61. Li, J. L., Wang, J. P., Chang, H., Deng, S. M., Du, J. H., Wang, X. X., ... & Song, Y. H. (2019). FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells. Cancer medicine.
62. Wang, T., Du, Q., Niu, Y., Zhang, X., Wang, Z., Wu, X., ... & Huang, Y. (2019). Cellular p32 Is a Critical Regulator of Porcine Circovirus Type 2 Nuclear Egress. Journal of virology, 93(23).
63. Wang, T., Duan, Y. M., Fu, Q., Liu, T., Yu, J. C., Sui, Z. Y., ... & Wen, G. Q. (2019). IM-12 activates the Wnt–β-catenin signaling pathway and attenuates rtPA-induced hemorrhagic transformation in rats after acute ischemic stroke. Biochemistry and Cell Biology, (999), 1-7.

64.  Du, Y., Du, Y., Zhang, Y., Huang, Z., Fu, M., Li, J., ... & He, G. (2019). MKP-1 reduces Aβ generation and alleviates cognitive impairments in Alzheimer’s disease models. Signal Transduction and Targeted Therapy, 4(1), 1-12.

65. Xie, X., Yu, C., Ren, Q., Wen, Q., Zhao, C., Tang, Y., & Du, Y. (2019). Exposure to HBCD promotes adipogenesis both in vitro and in vivo by interfering with Wnt6 expression. Science of The Total Environment, 135917.
66. Jiang, H., Li, R., Zhang, Z., Chang, C., Liu, Y., Liu, Z., ... & Wang, Q. Retinoid X receptor α (RXRα)‐mediated erythroid‐2‐related factor‐2 (NRF2) inactivation contributes to N, N‐dimethylformamide (DMF)‐induced oxidative stress in HL‐7702 and HuH6 cells. Journal of Applied Toxicology.
67. Dou, Y. Q., Kong, P., Li, C. L., Sun, H. X., Li, W. W., Yu, Y., ... & Dong, C. (2020). Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia. Theranostics, 10(3), 1197. Dou, Y. Q., Kong, P., Li, C. L., Sun, H. X., Li, W. W., Yu, Y., ... & Dong, C. (2020). Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia. Theranostics, 10(3), 1197
68. Li, C. L., Liu, B., Wang, Z. Y., Xie, F., Qiao, W., Cheng, J., ... & Liu, D. S. (2020). Salvianolic acid B improves myocardial function in diabetic cardiomyopathy by suppressing IGFBP3. Journal of Molecular and Cellular Cardiology.
69. Sakata, J., Hirosue, A., Yoshida, R., Matsuoka, Y., Kawahara, K., Arita, H., ... & Gohara, S. (2020). Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma. Cancers, 12(2), 494.
70. Du, X., He, W., He, H., & Wang, H. (2020). Beta-catenin inhibits bovine parainfluenza virus type 3 replication via innate immunity pathway. BMC Veterinary Research, 16(1), 1-10.
71. Li, C. L., Liu, X. H., Qiao, Y., Ning, L. N., Li, W. J., Sun, Y. S., ... & Ma, C. M. (2020). Allicin alleviates inflammation of diabetic macroangiopathy via the Nrf2 and NF-kB pathway. European Journal of Pharmacology, 173052.
72. Tong, S. M., Wang, D. Y., Cai, Q., Ying, S. H., & Feng, M. G. (2020). Opposite Nuclear Dynamics of Two FRH-Dominated Frequency Proteins Orchestrate Non-Rhythmic Conidiation in Beauveria bassiana. Cells, 9(3), 626.
73. Tang, S. Y., Liu, D. X., Li, Y., Wang, K. J., Wang, X. F., Su, Z. K., ... & Chen, Y. H. (2020). Caspr1 Facilitates sAPPα Production by Regulating α-Secretase ADAM9 in Brain Endothelial Cells. Frontiers in Molecular Neuroscience, 13, 23.
74. Wei, R., Bao, W., He, F., Meng, F., Liang, H., & Luo, B. (2020). Pannexin1 channel inhibitor (10panx) protects against transient focal cerebral ischemic injury by inhibiting RIP3 expression and inflammatory response in rats. Neuroscience.
75. Duan, Y., Wang, Z., Xu, L., Sun, L., Song, H., Yin, H., & He, F. (2020). lncRNA SNHG3 acts as a novel Tumor Suppressor and regulates Tumor Proliferation and Metastasis via AKT/mTOR/ERK pathway in Papillary Thyroid Carcinoma. Journal of Cancer, 11(12), 3492-3501.