{"title":"All Products","description":"\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/IVB_Banner_1916_x_190_px.png?v=1773328337\"\u003eBrowse all Invent Biotechnologies Products, including rapid protein extraction, cell fractionation, and single nuclei isolation kits. Designed with patented spin column technology enabling fast and reliable sample preparation from cells, tissues, plants, and more. Our kits simplify complex workflows, enabling you to obtain high-quality results quickly.\u003c\/p\u003e","products":[{"product_id":"minute-total-protein-extraction-kit-for-animal-cultured-cells-and-tissues-us-patent-9-580-462","title":"Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)","description":"\u003ch2\u003e\n\u003cspan style=\"font-weight: 400;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/1-Sd-001-SN-002_v8.pdf?v=1752160057\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1.pdf?12490201953603370455\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe height=\"315\" width=\"560\" src=\"https:\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" title=\"YouTube video player\"\u003e\u003c\/iframe\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/news-1\/ripa-buffer-the-potential-troubles\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003ca href=\"https:\/\/inventbiotech.com\/pages\/ripa-buffer-the-potential-troubles\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Cell_Sensitization_1.5_x_1.5_in.png?v=1752241598\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"110\" height=\"110\"\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cp\u003eThe Minute™ kit is a next-generation, spin column–based system designed for rapid and efficient total protein extraction from animal cultured cells and tissues. Unlike conventional solution-based methods, such as RIPA buffer, which often result in protein loss and altered profiles, this patented technology preserves protein integrity and yields reproducible results.\u003c\/p\u003e\n\u003cp\u003eWith enhanced lysis buffers and a streamlined protocol, proteins can be extracted in just 1–5 minutes at concentrations ranging from 1–8 mg\/ml. The system supports both denaturing and native conditions, allowing users to choose based on downstream applications. Minimal extraction volume (as low as 20 µl) makes it ideal when starting material is limited.\u003c\/p\u003e\n\u003cp\u003eThis kit offers one of the fastest protocols available for total protein preparation and can be readily adapted for high-throughput workflows. The extracted proteins are also suitable as starting material for small-scale purification using column chromatography.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/ripa-buffer-the-potential-troubles-1\"\u003e-RIPA Buffer - The Potential Troubles\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/pitfalls-of-protein-extraction-by-ripa-buffer-1\"\u003e-Pitfalls of Protein Extraction by RIPA\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/news-1\/a-technical-breakthrough-for-protein-sample-preparation\"\u003e-A Technical Breakthrough for Protein Sample Preparation\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eSelected references related to protein loss by RIPA buffer:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eBai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048\u003c\/li\u003e\n\u003cli\u003eMukhopadhyay, C. et al. (2016) PNAS 5:8228-8237\u003c\/li\u003e\n\u003cli\u003eLi, Q. (2016) Biotechniques. 61:327\u003c\/li\u003e\n\u003cli\u003eNgoka, L. CM. (2008) Proteome Science. 6:30\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_A_3600ed44-960b-492c-9588-9166e605dcf6_large.jpg?v=1540501877\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_B_12cc4294-e3c3-44fd-9dc4-b06d38768b63_large.jpg?v=1540501895\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003eProtein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver. \u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_SN-002_D_aea4bcc2-c65b-4095-a711-cc8f429e95cd_large.jpg?v=1540501948\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-001_Fish-GPDH-F_copy.jpg?836\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/RIPA_pellet_large.jpg?v=1555514187\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eKit includes:\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eItems\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eQuantity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Cell Lysis Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Cell Lysis Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: SD-001\/SN-002","offers":[{"title":"50 Preps","offer_id":16720730488943,"sku":"Cat #: SD-001\/SN-002","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SD-001_SN-002_1024x1024_4f04fd1f-0448-4d47-a2d7-7547a3477a81.png?v=1569161589"},{"product_id":"minute-detergent-free-protein-extraction-kit-for-animal-cultured-cells-and-tissues","title":"Minute™ Detergent-Free Protein Extraction Kit for Animal Tissues and Cultured Cells (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/6-SN-006_v3.pdf?v=1613417671\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-2.pdf?3563366020242764857\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Detergent-Free Total Protein Extraction Kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly extract detergent-free total proteins from cultured cells (insect\/mammalian\/other cultured cells) and animal tissues. The protein extraction buffers do not contain any detergent and EDTA. The extraction volume can be as small as 20 μl and as large as 500 μl with the use of the protein extraction filter cartridges. When available starting material is a limiting factor, this can be quite useful. Detergent-free total proteins can be extracted from cultured cells\/tissues in less than 5 minutes with high yield (1-5 mg\/ml).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SN-006_large.jpg?v=1540503291\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1: Drosophila cultured cells (SL2); Lane 2: Adult Drosophila flies; Lane 3: Gold fish muscle; Lane 4: Mouse liver. Extracted proteins were seperated in 10% SDS-PAGE and stained with Comassie blue.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Cap\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (8)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eSeo, J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002\/adhm.201400173\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eM. Takada, S. Chiba et al. ((2015). Inflammatory responses to neutral fat and fatty acids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International http:\/\/dx.doi.org\/10.1016\/j.forsciint.2015.07.011\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSeo J. et al. (2016). Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039\/C6RA03967G.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eXu, Wenteng, et al. (2016).\"Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination.\" Gene.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eYamamoto, H., Hayano, S., Okuno, Y., Onoda, A., Kato, K., Nagai, N., ... \u0026amp; Kato, T. (2020). Phosphorylated proteome analysis of a novel germline ABL1 mutation causing an autosomal dominant syndrome with ventricular septal defect. \u003cem\u003eInternational Journal of Cardiology\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003ePietras, P., Leśniczak-Staszak, M., Kasprzak, A., Andrzejewska, M., Jopek, K., Sowiński, M., ... \u0026amp; Szaflarski, W. (2021). MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells. \u003cem\u003eInternational Journal of Molecular Sciences\u003c\/em\u003e, \u003cem\u003e22\u003c\/em\u003e(22), 12121.\u003c\/li\u003e\n\u003cli\u003eMinerva, D., Othman, N. L., Nakazawa, T., Ito, Y., Yoshida, M., Goto, A., \u0026amp; Suzuki, T. (2022). A New Chemotactic Mechanism Governs Long-Range Angiogenesis Induced by Patching an Arterial Graft into a Vein. \u003cem\u003eInternational Journal of Molecular Sciences\u003c\/em\u003e, \u003cem\u003e23\u003c\/em\u003e(19), 11208.\u003c\/li\u003e\n\u003cli\u003eLeśniczak-Staszak, M., Pietras, P., Ruciński, M., Johnston, R., Sowiński, M., Andrzejewska, M., ... \u0026amp; Szaflarski, W. (2024). Stress granule-mediated sequestration of EGR1 mRNAs correlates with lomustine-induced cell death prevention. \u003ci style=\"font-family: 'Times New Roman', serif; font-size: 12pt;\"\u003eJournal of Cell Science\u003c\/i\u003e\u003cspan style=\"font-family: 'Times New Roman', serif; font-size: 12pt;\"\u003e, \u003c\/span\u003e\u003ci style=\"font-family: 'Times New Roman', serif; font-size: 12pt;\"\u003e137\u003c\/i\u003e\u003cspan style=\"font-family: 'Times New Roman', serif; font-size: 12pt;\"\u003e(12).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: SN-006","offers":[{"title":"50 Preps","offer_id":16742689898607,"sku":"Cat #: SN-006","price":473.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SN-006_1024x1024_bbe91c11-11d1-488a-b83f-16f0e3d3a5cc.png?v=1540328942"},{"product_id":"minute-cytoplasmic-nuclear-extraction-kits-for-cell","title":"Minute™ Cytoplasmic and Nuclear Extraction Kits for Cells (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/3-SC-003_v8.pdf?v=1678298205\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2.pdf?8070395893059634299\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Cytoplasmic and Nuclear Extraction Kit for Cells comprises optimized cytoplasmic extraction buffer, nuclear protein extraction buffer, and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plants, bacteria, yeast, and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SC-003_large.jpg?v=1540559695\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eCytoplasmic Extraction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eNuclear Extraction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eReferences (262)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eKalabus, JL. et al. (2012). MicroRNAs differentially regulate carbonyl reductase 1 (CBR1) gene expression dependent on the allele status of common polymorphic variant rs9024. PLOS ONE. 7(11):1-9.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eLombrafia AQ. et al (2014). Interindividual variability in the cardiac expression of anthracycline reductases in donors with and without down syndrome.  Pharm Res. 31:1644-1655.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eHou, N., et al. (2014). Vitamin A deficiency impairs spatial learning and memory: The mechanism of abnormal CBP-dependent histone acetylation regulated by retinoic acid receptor alpha. Mol Neurobiol.  DOI 10.1007\/s12035-014-8741-6.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eYou, Y., et al. (2014). Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3. Cell Death and Disease. (2014)5, e1493; doi: 10.1038\/cddis. 2014.443.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eTanaka M et al. (2016). \u003cem\u003eEx vivo \u003c\/em\u003eeffects of naphthoquinones allergen-sensitized mononuclear cells in mice. International Journal of Immunopathology and Pharmacology. DOI: 10.1177\/0394632016632221\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eLiu W. et al. (2016) Electroacupunctre improves motor impairment via inhibition of microglia-mediated neuroinflammation in the sensorimotor cortex after ischemic stroke. Life Sciences. \u003cu\u003edoi:10.1016\/j.lfs.2016.01.045\u003c\/u\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eXu, Yang, et al. (2016), RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF.\" \u003cem\u003eScientific Reports\u003c\/em\u003e 6:29362.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eShi, Le, et al. (2016). \"Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis.\" \u003cem\u003eTumor Biology\u003c\/em\u003e: 1-13.9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eHuang, C. W., Feng, W., Peh, M. T., Peh, K., Dymock, B. W., \u0026amp; Moore, P. K. (2016). A novel slow-releasing hydrogen sulfide donor, FW1256, exerts anti-inflammatory effects in mouse macrophages and in vivo. \u003cem\u003ePharmacological Research\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eMatsuoka, Y., Nakayama, H., Yoshida, R., Hirosue, A., Nagata, M., Tanaka, T., ... \u0026amp; Shinriki, S. (2016). IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. \u003cem\u003eBritish Journal of Cancer\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e \u003c\/strong\u003eMeng, C., Cui, X., Qi, S., Zhang, J., Kang, J., \u0026amp; Zhou, H. (2016). Lung inflation with hydrogen sulfide during the warm ischemia phase ameliorates injury in rat donor lungs via metabolic inhibition after cardiac death. \u003cem\u003eSurgery\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003eLiu, Y., Peng, J., Sun, T., Li, N., Zhang, L., Ren, J., ... \u0026amp; Ding, Y. (2017). Epithelial EZH2 serves as an epigenetic determinant in experimental colitis by inhibiting TNFα-mediated inflammation and apoptosis. \u003cem\u003eProceedings of the National Academy of Sciences\u003c\/em\u003e, 201700909.\u003c\/li\u003e\n\u003cli\u003eChen, Y., Hu, X., Guo, C., Yu, Y., \u0026amp; Lu, H. (2017). Epe1 contributes to activation of AMPK by promoting phosphorylation of AMPK alpha subunit, Ssp2. \u003cem\u003eScientific Reports\u003c\/em\u003e, \u003cem\u003e7\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003eHu, H., Zhu, W., Qin, J., Chen, M., Gong, L., Li, L., ... \u0026amp; Ye, D. (2016). Acetylation of PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. \u003cem\u003eHepatology\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003eHou, Y., Wang, Y., He, Q., Li, L., Xie, H., Zhao, Y., \u0026amp; Zhao, J. (2017). Nrf2 Inhibits NLRP3 Inflammasome Activation Through Regulating Trx1\/TXNIP Complex in Cerebral Ischemia Reperfusion Injury. \u003cem\u003eBehavioural Brain Research\u003c\/em\u003e.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e","brand":"Cat #: SC-003","offers":[{"title":"50 Preps","offer_id":16745372975215,"sku":"Cat #: SC-003","price":473.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SC-003_1024x1024_38c219f0-56fc-4f92-91fb-585d7596cc9a.png?v=1550161226"},{"product_id":"minute-total-protein-extraction-kit-for-plant-tissues","title":"Minute™ Total Protein Extraction Kit for Plant Tissues (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/8-SD-008-0SN-009_v5.pdf?v=1687456135\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_1ba26cd9-6f69-45bf-b9e9-ee267e712228.pdf?12885751298093300398\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Minute\u003csup\u003eTM\u003c\/sup\u003e total protein extraction kit for plant tissues comprises an optimized protein extraction buffer and protein extraction filter cartridges accompanied by 2.0 ml collection tubes. It aims to efficiently extract denatured or native proteins from various plant tissues, including leaves, seeds, soft stems, and roots. Notably, the protein profiles obtained through denaturing and native cell lysis buffers differ. Therefore, depending on the specific application, one buffer may outperform the other. This kit offers both denaturing and native cell lysis buffers, allowing users to evaluate and select the most suitable option for their particular needs. Utilizing the protein extraction filter cartridges makes it possible to extract total plant soluble proteins from 50-200 mg of plant tissue within 5-8 minutes while achieving a high protein yield ranging from 2-8 mg\/ml. This extraction method is useful for SDS-PAGE, immunoblotting, ELISA, IP, and enzyme assays. These extracted proteins can also be excellent starting materials for small-scale protein purification using column chromatography techniques.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SD-008_SN-009_large.jpg?v=1540565710\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eDenaturing Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eNative Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (340)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eCrystal R. et al. (2013). Expression profiling of four defense-related buffalograss transcripts in response to chinch bug (hemiptera: Blissidae) feeding. Journal of Economic Entomology. 106 (6):2568-2576.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWeber, C. F. (2017). Microgreen Farming and Nutrition: A Discovery-Based Laboratory Module to Cultivate Biological and Information Literacy in Undergraduates. The American Biology Teacher, 79(5), 375-386.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAbdulazeez, M., Abubakar, S. M., \u0026amp; Mu’azzam, J. M. (2017). Isolation and Characterization of a Potential Angiotensin-Converting Enzyme Inhibitory Peptide from the Leaves of Leptadenia hastata (Asclepiadaceae). Malaysian Journal of Applied Sciences, 2(1), 35-47.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShi, Z., Jiang, Y., Han, X., Liu, X., Cao, R., Qi, M., ... \u0026amp; Li, T. (2017). SlPIN1 regulates auxin efflux to affect flower abscission process. Scientific Reports, 7(1), 14919.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eOhno, S., Hori, W., Hosokawa, M., Tatsuzawa, F., \u0026amp; Doi, M. (2018). Post-transcriptional silencing of chalcone synthase is involved in phenotypic lability in petals and leaves of bicolor dahlia (Dahlia variabilis)‘Yuino’. Planta, 247(2), 413-428.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eJesus, F. G., Marchi-Werle, L., Fischer, H. D., Posadas, L. G., Graef, G. L., \u0026amp; Heng-Moss, T. Documenting Resistance and Physiological Changes in Soybean Challenged by Aphis glycines Matsumura (Hemiptera: Aphididae). Neotropical Entomology, 1-8.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLv, Z., Huang, Y., Ma, B., Xiang, Z., \u0026amp; He, N. (2018). LysM1 in MmLYK2 is a motif required for the interaction of MmLYP1 and MmLYK2 in the chitin signaling. Plant Cell Reports, 1-12.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMarchi-Werle, L., Fischer, H. D., Graef, G., Hunt, T. E., \u0026amp; Heng-Moss, T. M. (2018). Characterization and Identification of Methods for Phenotyping Soybean Populations With Tolerance to the Soybean Aphid (Hemiptera: Aphididae). Journal of Economic Entomology. toy177, https:\/\/doi.org\/10.1093\/jee\/toy177\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWang, J., Xia, H., Zhao, S. Z., Hou, L., Zhao, C. Z., Ma, C. L., ... \u0026amp; Li, P. C. (2018). A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis. \u003cem\u003eBiochemical and Biophysical Research Communications\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eChapman, K. M., Marchi-Werle, L., Hunt, T. E., Heng-Moss, T. M., \u0026amp; Louis, J. (2018). Abscisic and Jasmonic Acids Contribute to Soybean Tolerance to the Soybean Aphid (Aphis glycines Matsumura). \u003cem\u003eScientific Reports\u003c\/em\u003e, \u003cem\u003e8\u003c\/em\u003e(1), 15148.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SD-008\/SN-009","offers":[{"title":"50 Preps","offer_id":16745411575919,"sku":"Cat #: SD-008\/SN-009","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SD-008_SN-009_1024x1024_f568e808-699e-4437-8fcc-9fea60fff568.png?v=1540414577"},{"product_id":"minute™-detergent-free-plant-protein-extraction-kit","title":"Minute™ Detergent-Free Plant Protein Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/10-SN-010_v2.pdf?v=1613417689\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_36f11319-1faa-49d9-8da1-a8840c254786.pdf?8476546550085731236\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMinute™ Detergent-Free Plant Protein Extraction Kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from plant tissues. The protein extraction buffer does not contain any detergent or organic solvent.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe extraction volume can be as small as 50 μl and as large as 500 μl using the protein extraction filter cartridges. Detergent-free total proteins can be extracted from very small plant samples (leaves, seeds, soft stems, etc.) in less than 8 minutes with a high yield (1-6 mg\/ml).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Figure_010_large.jpg?v=1550163975\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDF-Lysis Buffer (with 5g beads)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: SN-010","offers":[{"title":"50 Preps","offer_id":16745442017391,"sku":"Cat #: SN-010","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SN-010_1024x1024_10ad9d47-bc0b-4797-a645-d2399834c39f.png?v=1540414730"},{"product_id":"minute™-total-protein-extraction-kit-for-mass-spectrometry","title":"Minute™ Total Protein Extraction Kit for Mass Spectrometry (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/26-MS-026_v3.pdf?v=1665164871\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-3.pdf?16867197103574543772\" target=\"_blank\"\u003eMSDS\u003cbr\u003e\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMass spectrometry has become a popular method for analyzing and characterizing proteins. Traditional total protein extraction methods, such as RIPA-based reagents, can alter endogenous baselines due to nonsystematic protein loss. Some chemicals in RIPA may not be compatible with mass spectrometry and other downstream applications. This kit extracts total proteins quickly using a specially formulated lysis buffer and proprietary spin column. The extracted proteins are compatible with trypsin digestion followed by MS analysis, iTRAQ, biotin labeling, etc. Due to the use of filter cartridges, the extraction volume can be as low as 20 µl – a helpful feature when starting material is a limiting factor. The typical yield is 2-8 mg\/ml.\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 60px;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MS-026-dataF_copy_ecf8f4e9-f59f-446f-ac9e-675c39b35dc3_large.jpg?v=1541008631\"\u003e\u003c\/div\u003e\n\u003cp\u003e \u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eLysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eChen, Y., Fang, Z., Zhou, J., Qin, H., \u0026amp; Ye, M. (2021). Mirror-Cutting-Based Digestion Strategy Enables the In-Depth and Accuracy Characterization of N-Linked Protein Glycosylation. \u003cem\u003eJournal of Proteome Research\u003c\/em\u003e.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: MS-026","offers":[{"title":"50 Preps","offer_id":16745461055599,"sku":"Cat #: MS-026","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/MS-026_1024x1024_583b2a25-f6be-4b32-9977-8aa93b87fb01.png?v=1540414859"},{"product_id":"minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes","title":"Minute™ Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/22-AT-022_v10.pdf?v=1650999022\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_435fc4ec-c7e0-4ad8-a74f-5f8537e5d08b.pdf?14248766210075683969\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eAdipose tissue, especially white adipose tissue (WAT), has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. Analyses of proteins from adipose tissues are increasingly critical for understanding many physiological\/pathological conditions. However, isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents. The water-oil emulsion present in a biological sample is notoriously difficult to separate. We have developed a novel technology to address this issue. A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effectively separate water-oil emulsion derived from adipose tissue homogenate. The extraction buffer has a lower freezing point than oil in adipose tissues, and the aqueous phase can be quickly separated from the oil phase by passing the tissue homogenate through the filter. The total proteins isolated are the unbiased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg\/ml) compared to traditional methods.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/protein-extraction-from-adipose-tissues-a-tough-challenge-1\" title=\"Protein Extraction from Adipose Tissues--- A Tough Challenge\" style=\"color: #ff8000;\" target=\"_blank\"\u003eProtein Extraction from Adipose Tissues--- A Tough Challenge\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/high-efficiency-protein-extraction-from-adipose-tissues-1\" title=\"High Efficiency Protein Extraction from Adipose Tissues\" style=\"color: #ff8000;\" target=\"_blank\"\u003eHigh-Efficiency Protein Extraction from Adipose Tissues\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/novel-method-for-high-quality-protein-extraction-from-adipose-tissues\" title=\"Novel Method for High Quality Protein Extraction from Adipose Tissues\" style=\"color: #ff8000;\" target=\"_blank\"\u003eNovel Method for High-Quality Protein Extraction from Adipose Tissues\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cimg style=\"font-size: 0.875rem;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AT-022_A_V2_large.jpg?v=1540918103\"\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AT-022_B_76908621-045f-4fe4-802d-81ae6c979dd5_large.jpg?v=1540504600\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA. SDS-PAGE (10%) profiles of total protein extracted from different adipose tissues. Lane 1, porcine WAT; Lane 2, chicken BAT; Lane 3, rat WAT.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eB. Western blottings of extracted proteins from rat WAT. Proteins were separated in 8-16% gradient SDS-PAGE and probed with following cellular protein marker antibodies:\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003eAnti-Na\/K ATPase alpha1, a plasma membrane marker (Upstate, clone 464.6), anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA), anti-ubiquinol-cytochrome C reductase core protein (abcam, ab 96333) and GAPDH, a cytosolic marker (Sigma). The specific protein bands were visualized by a substrate Opti-4CN (Bio-RAD).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Kit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable height=\"325\" width=\"540\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"text-align: center; height: 33px; width: 424px;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eBuffer A (Extraction Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003eBuffer B (10 X Denaturing Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003eBuffer C (10 X Non-Denaturing Buffer)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"height: 33px; width: 424px;\"\u003e\n\u003cp\u003e1.5 ml Microfuge Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 33px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21.3542px;\"\u003e\n\u003ctd style=\"height: 21.3542px; width: 424px;\"\u003e\n\u003cp\u003ePestles for 1.5 ml Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 21.3542px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 32px;\"\u003e\n\u003ctd style=\"height: 32px; width: 424px;\"\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 32px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 32px;\"\u003e\n\u003ctd style=\"height: 32px; width: 424px;\"\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 32px; width: 105.6px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: AT-022","offers":[{"title":"20 Preps","offer_id":16745493528687,"sku":"Cat #: AT-022","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/AT-022_1024x1024_7f058af1-f077-4669-86a5-79d0f6888557.png?v=1550164740"},{"product_id":"minute-bacterial-total-protein-extraction-kits","title":"Minute™ Bacterial Total Protein Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/4-SB-004_V3.pdf?v=1613417656\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-2_7822fd47-5c8e-4867-97d7-4f7515698944.pdf?10234070324513389564\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Bacterial Total Protein Extraction Kit is composed of optimized denaturing cell lysis buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly extract denatured proteins from bacteria. It contains sufficient materials for the extraction of total proteins from a 100 ml \u003c\/span\u003e\u003cem\u003e\u003cspan style=\"font-weight: 400;\"\u003eE. coli \u003c\/span\u003e\u003c\/em\u003e\u003cspan style=\"font-weight: 400;\"\u003eculture.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SB-004_V2_large.jpg?v=1540918155\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBacterial Lysis Buffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: left;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e2.5 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (2)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003ehttp:\/\/bio.lonza.com\/uploads\/tx_mwaxmarketingmaterial\/Lonza_Newsletter_2013-09_Resource_Notes_Fall.pdf#page=9\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eYonezawa, H., Osaki, T., Fukutomi, T., Hanawa, T., Kurata, S., Zaman, C., ... \u0026amp; Kamiya, S. (2017). Diversification of the AlpB outer membrane protein of Helicobacter pylori affects biofilm formation and cellular adhesion. Journal of Bacteriology, 199(6), e00729-16.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: SB-004","offers":[{"title":"50 Preps","offer_id":16745547399279,"sku":"Cat #: SB-004","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SB-004_1024x1024_51ae3623-a33b-4596-a210-5e54747760fc.png?v=1540415342"},{"product_id":"minute-cytosolic-and-nuclear-extraction-kit-for-frozen-fresh-tissues","title":"Minute™ Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/32-NT-032_v4.pdf?v=1613417826\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5.pdf?9155730713267963513\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePreparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. The majority of current commercial kits are designed to treat cultured cells and tissues similarly, not paying sufficient attention to the structural difference between the two. As a result, cross-contamination of cytosolic and nuclear fractions from fresh\/frozen tissues remains a major problem (see references 1 and 2 below). This novel Cytosolic and Nuclear Extraction Kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contamination for fresh and frozen tissue samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NT-032_large.jpg?v=1540559798\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer D\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer N\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e1.5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestle for 1.5 ml Tube\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e2 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e","brand":"Cat #: NT-032","offers":[{"title":"20 Preps","offer_id":16745574006895,"sku":"Cat #: NT-032","price":473.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/NT-032_1024x1024_7605e1e0-b8a3-48e7-8fcb-bec39d730313.png?v=1540415579"},{"product_id":"minute-total-protein-extraction-kit-for-microbes-with-thick-cell-walls","title":"Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)","description":"\u003ch2 style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/15-YT-015_v2.pdf?v=1613417717\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_2ea756a3-1c87-4216-b33d-b8d140bc6412.pdf?5055565149584538350\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YT-015 provides an instrument-free, fast and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include yeast, filamentous fungus, gram-positive and -negative bacteria, insect eggs, microalgae and more. This kit contains optimized denaturing and native protein extraction buffers to choose from. Unlike many other methods with harsh conditions, such as 8 M urea, glass bead lysis using an instrument and boiling the sample with alkaline extraction, etc., are used for yeast protein extraction. Proteins in certain molecular weight ranges are usually preferentially extracted with these methods. This kit features a single tube protocol and allows for a full range of yeast proteins to be extracted without bias. The kit works well for both log-phase and stationary-phase microbes. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-5 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/YT-015_large.jpg?v=1540504784\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDenaturing Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eNative Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Powder\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e5 grams\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e1.5 ml Microfuge Tube\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePestles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (10)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eNagayoshi, Y., Miyazaki, T., Shimamura, S., Nakayama, H., Minematsu, A., Yamauchi, S., ... \u0026amp; Mukae, H. (2017). Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species. PloS one, 12(7), e0180990.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAl-Omari, A., Griffith, J., Caranica, C., Taha, T., Schuttler, H. B., \u0026amp; Arnold, J. (2018). Discovering regulators in Post-Transcriptional control of the Biological Clock of Neurospora crassa using Variable Topology Ensemble Methods on GPUs. \u003cem\u003eIEEE access\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTerashima, M., Ohashi, K., Takasuka, T. E., Kojima, H., \u0026amp; Fukui, M. Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light‐enhanced growth and expresses putative photoactive proteins. \u003cem\u003eEnvironmental Microbiology Reports\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLin, W., Yuan, D., Deng, Z., Niu, B., \u0026amp; Chen, Q. The cellular and molecular mechanism of glutaraldehyde‐didecyldimethylammonium bromide as a disinfectant against Candida albicans. \u003cem\u003eJournal of Applied Microbiology\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShimamura, S., Miyazaki, T., Tashiro, M., Takazono, T., Saijo, T., Yamamoto, K., ... \u0026amp; Mukae, H. (2019). Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata. \u003ci\u003eFrontiers in microbiology\u003c\/i\u003e, \u003ci\u003e10\u003c\/i\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eWang, Y., Ma, Y., Wang, D., Liu, W., Chen, J., Jiang, Y., ... \u0026amp; Qin, L. (2019). Polar tube structure and three polar tube proteins identified from Nosema pernyi. \u003cem\u003eJournal of Invertebrate Pathology\u003c\/em\u003e, 107272.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eWang, L., Liu, J., Filipiak, M., Mungunkhuyag, K., Jedynak, P., Burczyk, J., ... \u0026amp; Malec, P. (2021). Fast and efficient cadmium biosorption by Chlorella vulgaris K-01 strain: The role of cell walls in metal sequestration. \u003c\/span\u003e\u003ci\u003eAlgal Research\u003c\/i\u003e\u003cspan\u003e, \u003c\/span\u003e\u003ci\u003e60\u003c\/i\u003e\u003cspan\u003e, 102497.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eFeng, K., Wang, W., Rong, J., Liang, J., Mi, J., Wu, Y., \u0026amp; Wang, Y. (2022). Construction of recombinant Pichia pastoris strains for ammonia reduction by the gdhA and glnA regulatory genes in laying hens. \u003cem\u003eEcotoxicology and Environmental Safety\u003c\/em\u003e, \u003cem\u003e234\u003c\/em\u003e, 113376.\u003c\/li\u003e\n\u003cli\u003eDorbani, I., Armengaud, J., Carlin, F., \u0026amp; Duport, C. (2023). Proteome of spores from biological indicators in sterilization processes: Bacillus pumilus and Bacillus atrophaeus. \u003cem\u003eProteomics\u003c\/em\u003e, 2300293.\u003c\/li\u003e\n\u003cli\u003eWang, Z., Zhong, S., Zhang, S., Zhang, B., Zheng, Y., Sun, Y., ... \u0026amp; Liu, X. (2024). A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ePLoS pathogens\u003c\/em\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003e20\u003c\/em\u003e(4), e1012138.\u003c\/li\u003e\n\u003cli\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: YT-015","offers":[{"title":"50 Preps","offer_id":16745580036207,"sku":"Cat #: YT-015","price":342.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/YT-015_1024x1024_3d2120cd-30c2-40f8-9276-a83492941149.png?v=1540415511"},{"product_id":"minute-detergent-free-protein-extraction-kit-for-microbes-with-thick-cell-walls","title":"Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/16-YD-016_v3.pdf?v=1613417725\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e | \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-2_2d9ecd29-6e0b-4220-b2f7-11545c0b0545.pdf?6318881280779245387\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include yeast, filamentous fungus, gram-positive and -negative bacteria, insect eggs, microalgae and more. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 minutes and the protein yield is in the range of 2-4 mg\/ml with unbiased protein representation. This kit contains a single-tube protocol and works well for both log-phase and stationary-phase microbes. The materials provided are sufficient for 50 extractions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/YD-016_large.jpg?v=1540504869\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Extraction Powder\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e5 grams\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e1.5 ml Microfuge Tube\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePestles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003ePeng, G., Hou, X., Zhang, W., Song, M., Yin, M., Wang, J., ... \u0026amp; Li, X. (2018). Alkyl rhamnosides, a series of amphiphilic materials exerting broad-spectrum anti-biofilm activity against pathogenic bacteria via multiple mechanisms. \u003cem\u003eArtificial cells, nanomedicine, and biotechnology\u003c\/em\u003e, 1-16.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: YD-016","offers":[{"title":"50 Preps","offer_id":16745590849647,"sku":"Cat #: YD-016","price":342.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/YD-016_1024x1024_2d645156-9c3e-4c6d-ba48-f83ef7c69283.png?v=1540415774"},{"product_id":"protein-extraction-kit-for-hair-and-nails","title":"Protein Extraction Kit for Hair and Nails (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/21-HD-021_v2.pdf?v=1613417755\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-2_dbcf5e74-83c9-4c76-9da2-6152f641b85f.pdf?12128983221100347587\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProteins account for about 80% of keratinized animal tissues such as hair, nail, horn and wool. These tissues contain mainly hard keratins made of several distinct types of protein with a molecular weight ranging from 10 to 135 kDa. The extraction buffer usually contains a high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective, but because of larger extraction buffer\/tissue ratio, the concentration of extracted protein is usually low. Extracted proteins need to be concentrated prior to further analysis. The presence of high-concentration denaturants may also interfere with downstream application. HD-021 is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants. The extracted protein can be directly loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other chemicals. Extracted protein concentration is 1-2 mg\/ml.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/HD-021_V2_large.jpg?v=1540918277\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e1.5 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eFilter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Cap\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 units\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (7)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eLee, S. H., Baldina, E., Lee, E., \u0026amp; Youm, Y. (2021). Social connectedness and hair cortisol in community-dwelling older adults. \u003cem\u003eComprehensive Psychoneuroendocrinology\u003c\/em\u003e, 100053.\u003c\/li\u003e\n\u003cli\u003eMarsden, A. J., Riley, D. R., Birkett, S., Rodriguez-Barucg, Q., Guinn, B. A., Carroll, S., ... \u0026amp; Beltran-Alvarez, P. (2021). Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers. \u003cem\u003eAmino Acids\u003c\/em\u003e, 1-10.\u003c\/li\u003e\n\u003cli\u003eKim, W. J., Park, K. M., Park, J. T., Seo, E., An, S. K., Park, H. Y., \u0026amp; Lee, E. (2021). Sex-specific association of hair cortisol concentration with stress-related psychological factors in healthy young adults. \u003cem\u003eBiology of Sex Differences\u003c\/em\u003e, \u003cem\u003e12\u003c\/em\u003e(1), 1-7.\u003c\/li\u003e\n\u003cli\u003eKim, W. J., Park, K. M., Park, J. T., Seo, E., Bang, M., An, S. K., ... \u0026amp; Lee, E. (2021). Effect of Childhood Trauma on the Association Between Stress-Related Psychological Factors and Hair Cortisol Level in Young Adults. \u003cem\u003ePsychiatry Investigation\u003c\/em\u003e, \u003cem\u003e18\u003c\/em\u003e(11), 1131.\u003c\/li\u003e\n\u003cli\u003eLee, S., Park, J. T., Bang, M., An, S. K., Namkoong, K., Park, H. Y., \u0026amp; Lee, E. (2022). Theory of mind and hair cortisol in healthy young adults: the moderating effects of childhood trauma. \u003cem\u003eEuropean Journal of Psychotraumatology\u003c\/em\u003e, \u003cem\u003e13\u003c\/em\u003e(2), 2116826.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eFuse, N., Morita, S., \u0026amp; Matsue, Y. (2023). Age‐related hair denaturation related to protein carbonyls. \u003c\/span\u003e\u003ci data-mce-fragment=\"1\"\u003eInternational Journal of Cosmetic Science\u003c\/i\u003e\u003cspan data-mce-fragment=\"1\"\u003e.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eFuse, N., Morita, S., \u0026amp; Matsue, Y. (2023). Age‐related hair denaturation related to protein carbonyls. \u003cem\u003eInternational Journal of Cosmetic Science\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003eLi, Z., Kong, W., Park, H. Y., Koo, S. J., Bang, M., Park, J. T., ... \u0026amp; An, S. K. (2024). Association of hair cortisol concentration with brain‐derived neurotrophic factor gene methylation: The role of sex as a moderator. \u003cem\u003eStress and Health\u003c\/em\u003e, e3401.\u003c\/li\u003e\n\u003cli\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: HD-021","offers":[{"title":"20 Preps","offer_id":16745643671663,"sku":"Cat #: HD-021","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/HD-021_1024x1024_a0bd5b0d-ea3c-450f-a65d-032c49ecfad4.png?v=1540415915"},{"product_id":"minute-plasma-membrane-protein-isolation-and-cell-fractionation-kit","title":"Minute™ Plasma Membrane\/Protein Isolation and Cell Fractionation Kit (50 Preps)","description":"\u003ch2\u003e\n\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5-SM-005_V11.pdf?v=1750697274\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003c\/strong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_32b5f5b1-bc6f-4d57-8f53-173b07067285.pdf?881483754317527433\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003cbr\u003e\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFree of detergents and EDTA, the Minute™ kit is a next-generation solution for native plasma membrane (PM) isolation and cell fractionation. It offers exceptional convenience and consistency by eliminating the variability often seen with traditional methods such as homogenization, density gradient centrifugation, and two-phase partitioning.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e\u003cspan style=\"color: #e69138;\"\u003eHow it works:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/em\u003e cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied, resulting in a cell lysate containing ruptured cell membranes and intact nuclei. As a result, nuclear contamination is virtually eliminated. PM is further separated from the cell lysate (a mixture of crude membranes, intact nuclei, cytosol proteins, and organelles) by subsequent differential and density centrifugation with a regular tabletop microcentrifuge. 5 distinct cell fractions (total membrane, PM, cytosol, nucleus, and organelles) can be obtained. The procedure can be completed in less than 45 minutes.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #ff8000;\"\u003e\u003cstrong\u003e\u003ca style=\"color: #ff8000;\" href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/how-to-choose-your-membrane-protein-isolation-kits?_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0_pos=1\u0026amp;_psq=how+to+choose\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"How to Choose Membrane Protein Isolation Kits\" target=\"_blank\"\u003eHow to Choose Membrane Protein Isolation Kits\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg height=\"408\" width=\"544\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/2_815b9e95-7f48-4418-95e1-eee070746034_1024x1024.png?v=1638553149\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_A.jpg?v=1613705277\"\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eFigure 1. A. SDS-PAGE profiles of total cell lysate (TC) and isolated plasma membrane proteins (PM) from human and rat cultured cells. Lane 1, Human lung cancer cell A549 total cell lysate; Lane 2, Isolated PM of A549; Lane 3, PM of rat REL-6TN cells; Lane 4, Total cell lysate of rat REL-6TN cells.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Western blotting: Proteins in A were transferred to nitrocellulose membrane and probed with anti-Na\/K ATPase alpha1, a plasma membrane marker (Upstate, Clone 464.6), and anti-lamin B1, a nuclear envelope marker (ab16048, Abcam Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD).\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eC. Densitometry measurement of Na\/K ATPase alpha1 signals in B (TC vs. PM). Total cell lysates were extracted by Minute Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_B.jpg?v=1613705277\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 2 A. SDS-PAGE profiles of isolated total membrane proteins from mouse tissues (T=Total Cell Lysate, C=Cytosol Fraction, M= Total Membrane Fraction)\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Proteins shown in A were transferred to a nitrocellulose membrane and probed with rabbit-anti mouse pan-cadherin (ab6529, Abcam, Cambridge, MA), and anti-actin by Western blotting. The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). Signals of pan-cadherin (a 130 kDa plasma membrane marker) were significantly enhanced in total membrane protein fractions. Total cell lysates were extracted by Minute™ Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Plymouth, MN).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SM-005_C.jpg?v=1613705277\"\u003eFigure 3. A. SDS-PAGE profiles of total membrane protein fraction (TM) and isolated plasma membrane protein fraction from mouse tissues. \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eB. Western blotting of proteins in A were transferred to nitrocellulose membrane and probed with rabbit anti-cadherin (abcam, Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eC. Densitometry measurement of cadherin signals in B (TM vs. PM).\u003c\/p\u003e\n\u003cp\u003e  \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: SM-005","offers":[{"title":"50 Preps","offer_id":16745644097647,"sku":"Cat #: SM-005","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SM-005_1024x1024_a26abe5b-9b42-400f-87e8-71c928c68b05.png?v=1569162641"},{"product_id":"minute™-protein-extraction-kit-for-fixed-and-embedded-tissues","title":"Minute™ Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/25-FE-025_v5.pdf?v=1613417778\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_1d62fd01-1cc4-4392-ad23-1d47411074bc.pdf?1864820778526915379\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eFormalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potentially rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin-mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at a high temperature with or without an optional sonication step. These methods can be effective but are tedious and time-consuming (3-4 hours). Minute™ Protein Extraction Kit for Fixed and Embedded Tissues provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 2-3 mg\/ml.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/FE-025_A_large.jpg?v=1540559603\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/FE-025_B_large.jpg?v=1540559622\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eBuffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e10 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1.5 ml Microfuge Tubes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e20 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePestles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtein Extraction Powder\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 grams\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (3)\u003c\/h5\u003e\n\u003cp\u003e1. Gnanaguru, G., Wagschal, A., Oh, J., Saez-Torres, K. L., Li, T., Temel, R. E., ... \u0026amp; D’Amore, P. A. (2021). Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. \u003cem\u003eMolecular Therapy\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e2. Obi, E. N., Tellock, D. A., Thomas, G. J., \u0026amp; Veenstra, T. D. (2023). Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics. \u003cem\u003eBiomolecules\u003c\/em\u003e, \u003cem\u003e13\u003c\/em\u003e(1), 96.\u003c\/p\u003e\n\u003cp\u003e3.  Moriyama, R., Nakamura, S., Mitsui, I., Sugiyama, M., Fukui, H., Fukui, H., ... \u0026amp; Suzuki, J. (2023). Expression of SARS-CoV-2 entry molecules ACE2, NRP1, TMPRSS2, and FURIN in the reproductive tissues of male macaques.\u003c\/p\u003e","brand":"Cat #: FE-025","offers":[{"title":"20 Preps","offer_id":16745809051759,"sku":"Cat #: FE-025","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/FE-025_1024x1024_04d9e206-2947-4ed0-89a0-afeff012e0e7.png?v=1540417034"},{"product_id":"minute-plasma-membrane-protein-isolation-kit-for-plants","title":"Minute™ Plant Plasma Membrane\/Protein Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/5p-SM-005p_v4.pdf?v=1613417660\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_47c0cabc-5d71-4cb6-ad4c-c17eaf1cf725.pdf?18003124456286137432\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning. These relatively effective methods require ultracentrifugation and a large amount of starting material. The procedures are usually tedious and time-consuming. To overcome the shortcomings, we have developed this PM isolation kit. Plant tissues are first sensitized by buffer A, homogenized and then passed through a specialized filter cartridge that allows homogenates to pass through with a zigzag path. The cell membranes are ruptured into a range of predefined sizes during the process. Native plasma membranes are separated from a mixture of unruptured cells, nuclei, cytosol and organelles by subsequent differential centrifugation and density centrifugation without using ultracentrifugation. Due to the use of the same amount of starting material, defined centrifugal force and predefined duration in every experiment, the result is much more consistent with a high degree of PM protein enrichment. The procedure can be completed in about 1 hour.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SM-005-P","offers":[{"title":"50 Preps","offer_id":16745966567535,"sku":"Cat #: SM-005-P","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SM-005-P_1024x1024_6b89c76f-4411-40f9-8512-6bd5bf1500c2.png?v=1540418227"},{"product_id":"minute-nuclear-envelope-protein-extraction-kit","title":"Minute™ Nuclear Envelope Protein Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/13-NE-013_v4.pdf?v=1613417706\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_474bb5c4-37c6-495c-a8bc-04f0ef28790c.pdf?2522610818804796492\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure. Minute™ Nuclear Envelope Protein Extraction Kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultracentrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods that require large amounts of starting cells\/tissues, this kit starts with only 10-20 million cells, and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 minutes with a final yield of 10-50 µg protein\/sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NE-013_large.jpg?v=1540560127\"\u003e\u003c\/p\u003e\n\u003cp\u003eA. SDS-PAGE profiles and Western blottings of isolated mouse spleen cells. Lanes 1 and 2, total cell lysate; Lane 3, nuclear envelope.\u003cbr\u003e\u003cbr\u003eB. SDS-PAGE profiles and Western blottings of Rate (REL-6TN) and human (MCF-7) cell lines. Lane 1, REL-6TN total cell lysate; Lane 2, REL-6TN nuclear envelope. Lane 3, MCF-7 total cell lysate; Lane 4, MCF-7 nuclear envelope. Nuclear envelope marker Lamin B1 (ab 16048, ABcam, Cambridge MA) and GAPDH (G9545, Sigma) were visualized by a color metric substrate (Bio-Rad).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cimg height=\"415\" width=\"450\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Nuclear_M_copy-2.jpg?15511872066431867953\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"gs_citr\" tabindex=\"0\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: NE-013","offers":[{"title":"50 Preps","offer_id":16745978003567,"sku":"Cat #: NE-013","price":772.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/NE-013_1024x1024_6702a280-d8e8-4695-8226-ddeb8e7475d6.png?v=1540418444"},{"product_id":"minute-mitochondria-isolation-kit-for-mammalian-cells-and-tissues","title":"Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/7-MP-007_v8.pdf?v=1613417675\" target=\"_blank\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_cc6e6529-157d-42e6-bcc0-28a01aefb99d.pdf?3708518995734123533\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues comprises optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to protein extraction filter cartridges, the protein isolation procedure is simple and provides a high yield. The process can be completed in less than 30 minutes. Unlike many commercial mitochondria preparation kits, this kit offers a wide range of starting cells and isolated mitochondria. The buffers are detergent and EDTA-free. A Dounce homogenizer or a tissue blender is not needed. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MP-007_large.jpg?v=1540560197\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eFigure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute™ Mitochondria Isolation Kit. \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues, respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues, respectively.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048, a nuclear envelope marker protein. abcam, Cambridge, MA). \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e C. Densitometry measurement of mitochondrial marker signals.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 540px;\" height=\"246\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"text-align: center; width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 427.2px; height: 35px;\"\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 98.4px; height: 35px;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e","brand":"Cat #: MP-007","offers":[{"title":"50 Preps","offer_id":16746014376047,"sku":"Cat #: MP-007","price":615.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Minute_Mitochondria_Isolation_Kit_for_Mammalian_Cells_and_Tissues_50_Preps.png?v=1751903675"},{"product_id":"minute™-ab-enhancer","title":"Minute™ Antibody Enhancer (30 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-002_v2.pdf?v=1613418026\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-3_c00b19c8-0d6f-46fe-aaff-671d2e3a2e0e.pdf?2475757267449237865\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Antibody Enhancer is able to significantly increase the sensitivity of commonly used immunoassays such as Western blotting, ELISA and immunohistochemistry. The enhancer can reduce the amount of primary and secondary antibodies used due to higher antibody dilution employed. The signal intensity can be increased in immunostaining applications with chromogenic or fluorescent substrates. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-002_A_V2_large.jpg?v=1540918609\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-002_B_V2_large.jpg?v=1540918623\" alt=\"\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eAntibody Enhancer \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-002","offers":[{"title":"30 ml","offer_id":16746024697967,"sku":"Cat #: WA-002","price":184.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-002_1024x1024_68fcd42e-9806-48eb-9322-7d4fbfd71959.png?v=1540418608"},{"product_id":"minute™-wb-stripping-solution","title":"Minute™ Western Blot Stripping Solution (250 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-003_v2.pdf?v=1613418042\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4.pdf?11068621270890347450\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Western Blot Stripping Solution is designed to rapidly strip antibodies bound to antigens on Western blotting membranes using chemiluminescent substrate. One of the major advantages of chemiluminescent detection is high sensitivity and the ability to strip reagents from a blot and reprobe the same blot. Reprobing Western blots are very useful for optimizing the blotting conditions, such as testing different antibody dilution and membrane-blocking conditions. The key to this process is to use conditions that cause the release of the antibody from the antigen without causing a significant amount of antigen to be released from the membrane. During the stripping procedure, a small amount of antigen is inevitably lost from the membrane. It is important to minimize this loss by stripping the antibody under gentle conditions. Minute Western Blot Stripping Solution is able to achieve maximum removal of antibodies from a membrane and preserve the integrity of the antigen in 10-15 minutes without using harsh conditions such as boiling or treating with strong reducing agents. A blot can be stripped and reprobed multiple times to visualize multiple proteins or to optimize experimental conditions without the need for running multiple gels. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-003_V2_d83d6d33-aa20-48b9-92a2-fd1dbe6f2cba_large.jpg?v=1540919116\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eWestern Blot Stripping Solution \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e250 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-003","offers":[{"title":"250 ml","offer_id":16746045079663,"sku":"Cat #: WA-003","price":184.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-003_1024x1024_cf43a9ce-4c6f-418f-87bc-940b2e2c9fc0.png?v=1540418847"},{"product_id":"minute™-adipose-tissue-fractionation-kit","title":"Minute™ Adipose Tissue Fractionation Kit (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/23-AF-023_v3.pdf?v=1613417767\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_2b89818b-449e-4bb9-8bd8-7463f5af04f3.pdf?5912556579260951834\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eTotal cellular proteins account for less than 2% of adipose tissue. The white adipose tissue, in particular, has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. Fractionation and analysis of proteins from adipose tissue are critical for understanding many physiological\/pathological conditions. However, fractionation of adipose tissue is challenging due to its high lipid and low protein contents. The water-oil emulsion present in biological samples is notoriously difficult to separate. Still, we have developed a cutting-edge technology to deal with this issue: a porous filter with unique surface properties and predefined pore size coupling with a specially formulated detergent-free fractionation buffer. This is the key to fractionating adipose tissue into two: water-soluble protein fraction and water-insoluble fraction. The buffers used in this kit are free of primary amine, detergent, and reducing agents. Isolated proteins are compatible with all downstream applications, including TMT labeling, enzyme digestion, MS analysis, and other applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AF-023_large.jpg?v=1540560279\"\u003e\u003cbr\u003eA. SDS-PAGE (10%) profiles of fractionated adipose tissues. Lane 1, a water-soluble fraction of porcine WAT; Lane 2, a water-insoluble fraction of porcine WAT; Lane 3, a water-soluble fraction of rat WAT; Lane 4, a water-insoluble fraction of rat WAT. \u003cbr\u003e\u003cbr\u003eB. Western blottings of fractionated proteins from rat WAT; Lane 3. water-soluble fraction; Lane 4. Water-insoluble fraction. Proteins were separated in 10% SDS-PAGE and probed with the following cellular protein marker antibodies: Anti-Na\/K ATPase alpha1, a plasma membrane marker (Upstate, clone 464.6), anti-lamin B1, a nuclear envelope marker (ab16048, Abcam Cambridge, MA), anti-ubiquinol-cytochrome C reductase core protein (Abcam, ab 96333) and GAPDH, a cytosolic marker (Sigma). The specific protein bands were visualized by a substrate Opti-4CN (Bio-RAD). \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1.5 ml Microfuge Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestles for 1.5 ml Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge with Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (3)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eMarkan, K. R., Naber, M. C., Small, S. M., Peltekian, L., Kessler, R. L., \u0026amp; Potthoff, M. J. (2017). FGF21 resistance is not mediated by downregulation of beta-klotho expression in white adipose tissue. Molecular Metabolism. http:\/\/dx.doi.org\/10.1016\/j.molmet.2017.03.009\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLiu, X., Tong, W., Zhao, X., Zhang, H., Tang, Y., \u0026amp; Deng, X. Chinese herb extract improves liver steatosis by promoting the expression of high molecular weight adiponectin in NAFLD rats. Molecular Medicine Reports.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eSubbaramaiah, K., Iyengar, N. M., Morrow, M., Elemento, O., Zhou, X. K., \u0026amp; Dannenberg, A. J. (2018). Prostaglandin E2down-regulates sirtuin 1 (SIRT1) leading to elevated levels of aromatase, providing insights into the obesity-breast cancer connection.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eJournal of Biological Chemistry\u003c\/em\u003e, jbc-RA118.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: AF-023","offers":[{"title":"20 Preps","offer_id":16746062184559,"sku":"Cat #: AF-023","price":604.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/AF-023_1024x1024_febd893f-3712-45c1-826d-5c550037048b.png?v=1573145307"},{"product_id":"minute™-ponceau-s-stain","title":"Minute™ Ponceau S Stain (250 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-004_v2.pdf?v=1613418048\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-3_00e63da0-671c-4636-933f-68adafd08e35.pdf?15802959883295459718\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis is a rapid and reversible staining used for locating protein bands on Western blots and comparing protein band intensity. It produces reddish-pink-stained bands on membranes, which is useful because it does not have a deleterious effect on the property of proteins. It is also commonly used as a method for locating polypeptides for protein sequencing. The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes. It is not recommended for positively charged membranes such as nylon membranes. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-004_V2_1bcd06c7-36ec-4c05-b40b-25096aa23681_large.jpg?v=1540919157\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"margin-left: auto; margin-right: auto;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePonceau S Stain\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e250 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-004","offers":[{"title":"250 ml","offer_id":16746073358447,"sku":"Cat #: WA-004","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-004_1024x1024_f104b3ad-ea25-45bf-aee7-3318e69fba41.png?v=1540418953"},{"product_id":"minute-detergent-free-nuclei-isolation-kit","title":"Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/24-NI-024_v8_b4da52af-ee41-4e2b-8b6d-29b1cd3b02be.pdf?v=1645732137\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_198858e0-6493-427b-8682-369e42a2a92c.pdf?13345605419166362649\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #120a39;\"\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style=\"color: #410f0f;\"\u003e*Invent Biotechnologies, Inc. is the inventor of the\u003cspan\u003e \u003c\/span\u003espin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the\u003cspan\u003e \u003c\/span\u003etechnology.\u003c\/span\u003e \u003c\/em\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #120a39;\"\u003e\u003cstrong\u003eWatch the newest Video on JoVE\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ciframe id=\"embed-iframe\" height=\"260\" width=\"460\" border=\"0\" src=\"https:\/\/www.jove.com\/embed\/player?id=61471\u0026amp;s=1\u0026amp;fpv=1\"\u003e\u003c\/iframe\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSingle-cell genomics requires high-quality and complete genetic materials from individual cells. However, intact single cells are hard to isolate from tissues, especially when frozen. In addition, d\u003cspan\u003eissociation-induced (either mechanical or enzymatic) population and expression bias are also problematic. It is more feasible to isolate intact nuclei to be used as the starting material for most single-cell genomics applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-based technologies in less than 20 min without using tissue homogenizer and any detergents. The traditional method for nuclei isolation involves the use of non-ionic detergent, which has a tendency to cause unwanted nuclear aggregation. It is unclear why some nuclei are aggregated while others are not. It is also unknown whether non-aggregated nuclei are an unbiased representation of the whole nuclei population. Detergents also have the potential to damage the nuclear envelope resulting in leakage of nuclear matrix materials. For some cell\/tissue types, nuclear envelop proteins could also be stripped off by the detergents. Minute\u003csup\u003eTM \u003c\/sup\u003eDetergent-Free Nuclei Isolation Kit provides a whole new way for nuclei isolation in contrast to traditional methods.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHow it works:\u003c\/strong\u003e cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied. The cells are ruptured when passing through the filter leaving intact native nuclei in the flow-through. The nuclei are separated from other small cell debris by low-speed centrifugation using the proprietary buffer B. The native and intact nuclei isolated can be used for a variety of downstream applications that include but not limited to: FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and\/or apoptosis research.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFor storage and transportation of isolated single nuclei, the following buffer is recommended:\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1\u0026amp;_sid=8b67760c6\u0026amp;_ss=r\"\u003e\u003cem\u003eWA-014: Minute™ Anti-Clumping Nuclei Storage Buffer \u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e We also offer the following single nucleus isolation kits with a much cleaner background if the presence of detergent is not a concern:\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1\u0026amp;_sid=2bc6f4939\u0026amp;_ss=r\" target=\"_blank\"\u003eSN-047: Minute™ Single Nucleus Isolation Kit for Tissues\/Cells\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps?_pos=1\u0026amp;_sid=1f449fc5a\u0026amp;_ss=r\" target=\"_blank\"\u003eBN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues\/Cells\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-nuclei-and-cytosol-isolation-kit-for-adipose-tissues?_pos=1\u0026amp;_sid=f91e6c921\u0026amp;_ss=r\" target=\"_blank\"\u003eAN-029: Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cem\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NI-024-Zbfish_brain_480x480.jpg?v=1580321147\"\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eNuclei Isolated from zebrafish brain tissue Using NI-024 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Université Libre de Bruxelles).\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NI-024_A_large.jpg?v=1540560432\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/R-F_copy_large.jpg?v=1564769298\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NI-024_B_large.jpg?v=1540560450\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Monkeypsd_copy_large.jpg?v=1564769309\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/1_07680836-ed07-463b-8a43-059195a26cd6_large.jpg?v=1557247782\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NI-024_F_0e1cc6a6-df8a-4b2f-8c36-3b346b2ed40c_480x480.jpg?v=1568227047\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"text-align: center; width: 415px; height: 36px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 36px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 415px; height: 36px;\"\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 36px;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 415px; height: 36px;\"\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 36px;\"\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 415px; height: 36px;\"\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 36px;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 415px; height: 36px;\"\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 36px;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"width: 415px; height: 33px;\"\u003e\n\u003cp\u003ePlastic Rod\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 113px; height: 33px;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: NI-024","offers":[{"title":"20 Preps","offer_id":16746100392047,"sku":"Cat #: NI-024","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/NI-024_1024x1024_b3c22f55-3a1c-487d-91a9-8429e1eab38c.png?v=1569162666"},{"product_id":"minute-peroxidase-suppressor","title":"Minute™ Peroxidase Suppressor (30 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-005_v2.pdf?v=1613418055\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_2d367d76-6026-4e18-b8da-e72f5e9d5d27.pdf?12736583005252625971\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMinute™ Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positives and reducing the background of IHC. Many chemical agents such as H\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e₂\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003eO\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e₂\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e and sodium aside have been used for this purpose, but the inhibition is usually incomplete. Minute™ HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibition of endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super-sensitive procedures such as tyramide signal amplification (TSA).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eMajor Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduces the background of sensitive assays using HRP.\u003c\/p\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eAfter cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eBlock non-specific sites in the samples with normal serum or other blocking solution of your choice. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eAdd 1-2 drops of HRP suppressor to cover the samples on the slide. Incubate at room temperature in a humidity chamber for 10-15 minutes. Wash the sample on the slide 3 times with an IHC washing buffer such as PBS-tween and perform primary antibody incubation. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eIt is important that HRP suppressor must be added after antigen retrieval and before primary antibody incubation. It may interfere with antigen-antibody binding if it is not washed away after incubation.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eContinue with other experimental steps. \u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-005_V2_large.jpg?v=1540918666\" alt=\"\"\u003e\u003cbr\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePeroxidase Suppressor \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-005","offers":[{"title":"30 ml","offer_id":16746100883567,"sku":"Cat #: WA-005","price":132.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-005_1024x1024_dc7fd630-69a6-472a-a520-f7053739fef9.png?v=1540419078"},{"product_id":"minute™-yeast-mitochondria-enrichment-kit","title":"Minute™ Yeast Mitochondria Enrichment Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/17-YM-017_v7.pdf?v=1727889696\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_bddb7ea1-0347-4c40-b33a-551fb645725c.pdf?15893953067407627326\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eTraditional protocol for yeast mitochondria isolation\/extraction involves a range of centrifugation-based sub-cellular fractionation procedures. Typically, the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time-consuming. We feature a simple and efficient protocol for yeast mitochondria enrichment. The procedure is gentle and instrument free. Native mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 μg\/sample. The materials provided are sufficient for 50 extractions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/YM-017_large.jpg?v=1540560518\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/YM-17_Fig-2_JEP.jpg?v=1727886942\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eEnrichment of Mitochondria detected by Western Blotting. \u003cspan style=\"mso-spacerun: yes;\"\u003e \u003c\/span\u003ePonceau red stained blot (A) and Western blotting (B). Lane 1, Total yeast cell lysate; Lane 2, Cytosolic fraction; Lane 3 and 4, Mitochondrial fraction from two separated Experiments. Por1 is a mitochondrial marker antibody (Thermofisher, 16G9E6BC4).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e10 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1.5 ml Microfuge Tube\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e4 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eYamashita, K., Miyazaki, T., Fukuda, Y., Mitsuyama, J., Saijo, T., Shimamura, S., ... \u0026amp; Kohno, S. (2019). The novel arylamidine T-2307 selectively disrupts yeast mitochondrial function by inhibiting respiratory chain complexes. \u003cem\u003eAntimicrobial Agents and Chemotherapy\u003c\/em\u003e, AAC-00374.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: YM-017","offers":[{"title":"50 Preps","offer_id":16746125262959,"sku":"Cat #: YM-017","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/YM-017_1024x1024_1179634c-ec9a-4096-be09-300ee3fb293a.png?v=1540419234"},{"product_id":"minute-high-efficiency-protein-precipitation-kit","title":"Minute™ High-Efficiency Protein Precipitation Kit (30 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-006_v4.pdf?v=1700251448\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_fb35d81f-b75a-48a8-b2a1-ad421bd7eaf0.pdf?14355643214342510928\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eProtein precipitation stands out as a clear strategy for concentrating proteins and eliminating interfering substances present in protein samples, such as salts, lipids, and other elements that might disrupt subsequent applications. Among the widely employed techniques, trichloroacetic acid (TCA) precipitation emerges as a straightforward and efficient method. However, proteins precipitated through the TCA method typically undergo denaturation, often resulting in reduced solubility.\u003c\/p\u003e\n\u003cp\u003eA notable drawback of the conventional TCA\/acetone precipitation approach is its diminished efficacy when dealing with samples featuring low protein concentrations. Higher protein concentration samples are precipitated much more efficiently compared to those with lower protein concentrations. In response to these limitations, we have innovated a high-efficiency protein precipitation kit, a refinement of the traditional TCA method. This kit is characterized by its simplicity, rapidity, and heightened effectiveness. It enables the effective precipitation and concentration of proteins at low concentrations in less than 30 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-006_A_large.jpg?v=1540564679\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-006_B_large.jpg?v=1540564709\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable height=\"122\" width=\"534\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 398.562px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 119.438px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 398.562px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Precipitation Solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 119.438px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 398.562px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eWashing Solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 119.438px; text-align: center;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 398.562px;\"\u003eSolution P\u003c\/td\u003e\n\u003ctd style=\"width: 119.438px; text-align: center;\"\u003e6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-006","offers":[{"title":"30 ml","offer_id":16746131062895,"sku":"Cat #: WA-006","price":195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-006_1024x1024_de4c013e-3932-46c5-b5db-d81967a2f727.png?v=1540419341"},{"product_id":"minute-plant-microsomal-membrane-extraction-kit","title":"Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/18-MM-018_v4_087e1b44-c47e-46a9-be79-3d2c940e49e2.pdf?v=1616182195\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_fe90005d-d8a9-49dc-8acc-c5ac6e5a425c.pdf?15250503227495695386\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eIsolation of microsomal membranes from plant tissues is a common procedure. The microsomal fraction of plant cell lysate is the focus of interest in many plant research projects and is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes, and other components of a membrane system. Traditional methods for microsomal fraction isolation involve differential pelleting, where centrifugation steps are required for membrane fractions. Microsomal fraction isolation usually requires a large amount of starting material and has tedious ultracentrifugation steps. MM-018 offers a simple, fast, and user-friendly approach for microsomal membrane extraction with a small amount of starting material. During the procedures, water-soluble cytosolic proteins are removed, and water-insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a tabletop microcentrifuge. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg\/sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/MM-018_large.jpg?v=1540560592\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0 ml Collection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (4)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eKim, S., Choi, Y., Kwon, C., \u0026amp; Yun, H. S. (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721\/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. \u003cem\u003eJournal of integrative plant biology\u003c\/em\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" tabindex=\"0\"\u003eYang, Z., Yang, J., Wang, Y., Wang, F., Mao, W., He, Q., ... \u0026amp; Mao, C. (2020). PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. \u003ci\u003eThe Plant Cel\u003c\/i\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003eYu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., \u0026amp; Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. \u003ci data-mce-fragment=\"1\"\u003eMolecular Plant\u003c\/i\u003e, \u003ci data-mce-fragment=\"1\"\u003e13\u003c\/i\u003e(11), 1556-1569.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003eKato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., \u0026amp; Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. \u003cem\u003eCrop Science\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003eHou, L. Y., Lehmann, M., \u0026amp; Geigenberger, P. (2021). Thioredoxins o1 and h2 Show Different Subcellular Localizations and Redox-Active Functions, but Cooperatively Affect NADPH Redox Poise and Photosynthetic Performance in Fluctuating Light.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" data-mce-fragment=\"1\" tabindex=\"0\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003eChiu, C. Y., Tsai, C. D., Wang, J. Y., Tsai, M. H., Kanno, S., Lung, H. F., \u0026amp; Liu, T. Y. (2024). Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis. \u003ci\u003ebioRxiv\u003c\/i\u003e, 2024-06.\u003c\/span\u003e\u003cb style=\"mso-bidi-font-weight: normal;\"\u003e\u003cspan style=\"font-size: 12.0pt; mso-bidi-font-size: 11.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman';\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: MM-018","offers":[{"title":"50 Preps","offer_id":16746183065711,"sku":"Cat #: MM-018","price":699.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/MM-018_1024x1024_a5fcdb1a-c37a-4150-b786-f5fd2bcef696.png?v=1540419540"},{"product_id":"minute™-rbc-lysis-buffer","title":"Minute™ RBC Lysis Buffer (250 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-007_v2.pdf?v=1613418070\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-3_b0061c97-8fdc-4345-a1b5-78fa43eb623f.pdf?7178455364702486940\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eRed blood cells (RBC) are abundant in blood-rich organs and are the major component of blood. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe RBC are not desirable in protein and nucleic acid extraction from blood-rich organs and analysis of nucleated cells from blood by flow cytometry and should be removed before extraction. Minute™ RBC Lysis Buffer is a modified ammonium chloride solution that can effectively lyse RBC without a significant effect on other nucleated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-007_A_large.jpg?v=1540564759\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-007_B_V2_large.jpg?v=1540918770\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eRBC Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e250 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-007","offers":[{"title":"250 ml","offer_id":16746203086959,"sku":"Cat #: WA-007","price":174.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-007_1024x1024_102b2251-ca8d-4907-8535-833ff93ce670.png?v=1540419611"},{"product_id":"minute™-wb-blocking-solution","title":"Minute™ WB Blocking Solution (250 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-008_v2.pdf?v=1613418077\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_f38223c2-e2d1-47e3-a805-b3217af52c37.pdf?614588438018965228\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe WB blocking solution is designed to block non-specific binding of antibodies to WB blot membranes.  This proprietary formulated blocker is a non-casein and non-milk based solution that effectively blocks non-specific binding rapidly and effectively resulting in clear background, increased contrast and enhanced signal intensity.  This solution can also be used in ELISA, IHC and other detection system where blocking of non-specific antibody binding is needed.  This blocking solution does not contain detergent and Tris.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eWestern Blot Blocking Solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e250 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-008","offers":[{"title":"250 ml","offer_id":16746233954415,"sku":"Cat #: WA-008","price":174.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-008_1024x1024_b7312e18-f103-4299-9baf-26ab163c87f6.png?v=1540419698"},{"product_id":"minute-chloroplast-isolation-kit","title":"Minute™ Chloroplast Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/11-CP-11_v6.pdf?v=1613417694\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_e725b4e9-433b-4de1-a9b1-c8d0b59bbce2.pdf?14120686575185508492\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eInvent Biotechnologies Minute\u003csup\u003eTM \u003c\/sup\u003echloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 50-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 10\u003csup\u003e6\u003c\/sup\u003e to 1 X 10\u003csup\u003e7\u003c\/sup\u003e intact chloroplasts (\u0026gt;90% intact) from fresh plant leaves.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/CP-011_large.jpg?v=1540560637\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 358px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eBuffer A (with 5 grams dissociation beads)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cbr\u003e\n\u003c\/div\u003e\nBuffer B\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 358px;\"\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 78px;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: CP-011","offers":[{"title":"50 Preps","offer_id":16746252206191,"sku":"Cat #: CP-011","price":394.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/CP-011_1024x1024_628e6114-598c-40a1-a562-dc63bed29332.png?v=1540419828"},{"product_id":"minute™-denaturing-protein-solubilization-reagent","title":"Minute™ Denaturing Protein Solubilization Reagent (10 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-009_v2.pdf?v=1613418084\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e| \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_12b93a25-6281-4a31-a98c-4cea660a7107.pdf?1325044723049455505\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are denatured due to the presence of an anionic detergent (SDS). (Cat. No. WA-012, Invent Biotechnologies).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: left;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDenaturing Protein Solubilization Reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e10 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-009","offers":[{"title":"10 ml","offer_id":16746257481839,"sku":"Cat #: WA-009","price":84.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-009_1024x1024_9ae4bb2f-bafd-4879-ac3f-4503e544d146.png?v=1540419896"},{"product_id":"minute™-hi-efficiency-exosome-precipitation-reagent","title":"Minute™ High-Efficiency Exosome Precipitation Reagent (20 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/27-EI-027_v4.pdf?v=1613417790\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_f7370681-e27f-491c-a515-66994e4fd352.pdf?9439765861022550130\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eExosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm. The biological function of exosomes is believed to serve as intercellular messengers. Filtration and ultracentrifugation are classical ways of isolating and enriching exosomes. This is a tedious process and requires special equipment. Another common way for enrichment of exosome is through precipitation. Currently major commercial kits are PEG-based. EI-027 is designed to precipitate total exosomes from biofluids using a high efficacy, non-PEG based reagent for exosome precipitation. This kit is suitable for routine biofluid samples using the same reagent and similar protocol.\u003c\/p\u003e\n\u003cp\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/EI-027_large.jpg?v=1540560686\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eExosome Precipitation Reagent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e20 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (11)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eBai, H., Pan, Y., Qi, L., Liu, L., Zhao, X., Dong, H., ... \u0026amp; Wang, X. (2018). Development a hydrazide-functionalized thermosensitive polymer based homogeneous system for highly efficient N-glycoprotein\/glycopeptide enrichment from human plasma exosome. Talanta.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cdiv class=\"gs_citr\" tabindex=\"0\"\u003eJiao, F., Xia, C., Zhao, Y., Ying, W., Xie, Y., Guan, X., ... \u0026amp; Qian, X. (2018). A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2. \u003ci\u003eChemical Science\u003c\/i\u003e.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003eTan, Y., Nie, W., Chen, C., He, X., Xu, Y., Ma, X., ... \u0026amp; Wang, W. (2019). Mesenchymal stem cells alleviate hypoxia-induced oxidative stress and enhance the pro-survival pathways in porcine islets. \u003cem\u003eExperimental Biology and Medicine\u003c\/em\u003e, 1535370219844472.\u003c\/li\u003e\n\u003cli\u003eCheng, P., Feng, F., Yang, H., Jin, S., Lai, C., Wang, Y., \u0026amp; Bi, J. (2020). Detection and significance of exosomal mRNA expression profiles in the cerebrospinal fluid of patients with meningeal carcinomatosis. \u003cem\u003eJournal of Molecular Neuroscience\u003c\/em\u003e, 1-14.\u003c\/li\u003e\n\u003cli\u003eLu, T., Zhang, Z., Zhang, J., Pan, X., Zhu, X., Wang, X., ... \u0026amp; Yan, M. (2022). CD73 in small extracellular vesicles derived from HNSCC defines tumour‐associated immunosuppression mediated by macrophages in the microenvironment. \u003cem\u003eJournal of Extracellular Vesicles\u003c\/em\u003e, \u003cem\u003e11\u003c\/em\u003e(5), e12218.\u003c\/li\u003e\n\u003cli\u003eTheel, E. K., \u0026amp; Schwaminger, S. P. (2022). Microfluidic Approaches for Affinity-Based Exosome Separation. \u003ci data-mce-fragment=\"1\"\u003eInternational Journal of Molecular Sciences\u003c\/i\u003e, \u003ci data-mce-fragment=\"1\"\u003e23\u003c\/i\u003e(16), 9004.\u003c\/li\u003e\n\u003cli\u003eOmrani, M., Beyrampour-Basmenj, H., Jahanban-Esfahlan, R., Talebi, M., Raeisi, M., Serej, Z. A., ... \u0026amp; Ebrahimi-Kalan, A. (2023). Global trend in exosome isolation and application: an update concept in management of diseases. \u003cem\u003eMolecular and Cellular Biochemistry\u003c\/em\u003e, 1-13.\u003c\/li\u003e\n\u003cli\u003eOmrani, M., Beyrampour-Basmenj, H., Jahanban-Esfahlan, R., Talebi, M., Raeisi, M., Serej, Z. A., ... \u0026amp; Ebrahimi-Kalan, A. (2023). Global trend in exosome isolation and application: an update concept in management of diseases. \u003cem\u003eMolecular and Cellular Biochemistry\u003c\/em\u003e, 1-13.\u003c\/li\u003e\n\u003cli\u003eCheng, C., Wang, P., Yang, Y., Du, X., Xia, H., Liu, J., ... \u0026amp; Liu, Q. (2023). Smoking‐Induced M2‐TAMs, via circEML4 in EVs, Promote the Progression of NSCLC through ALKBH5‐Regulated m6A Modification of SOCS2 in NSCLC Cells. \u003cem\u003eAdvanced Science\u003c\/em\u003e, 2300953.\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-mce-fragment=\"1\"\u003eChen, G., Pu, G., Wang, L., Li, Y., Liu, T., Li, H., ... \u0026amp; Luo, X. (2023). Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits. \u003cem data-mce-fragment=\"1\"\u003eFrontiers in Immunology\u003c\/em\u003e, \u003cem data-mce-fragment=\"1\"\u003e14\u003c\/em\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eCheng, C., Wang, P., Yang, Y., Du, X., Xia, H., Liu, J., ... \u0026amp; Liu, Q. (2023). Smoking‐Induced M2‐TAMs, via circEML4 in EVs, Promote the Progression of NSCLC through ALKBH5‐Regulated m6A Modification of SOCS2 in NSCLC Cells. \u003cem\u003eAdvanced Science\u003c\/em\u003e, 2300953.\u003c\/li\u003e\n\u003cli\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: EI-027","offers":[{"title":"20 ml","offer_id":16746271539311,"sku":"Cat #: EI-027","price":394.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/EI-027_1024x1024_b0593b69-2e7a-4c5e-a4a2-20e2421333ea.png?v=1540420034"},{"product_id":"minute™-non-denatured-protein-solubilization-reagent","title":"Minute™ Non-Denatured Protein Solubilization Reagent (10 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-010_v2.pdf?v=1613418091\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_dc858d06-084a-4465-8be5-3e65e27b4eec.pdf?471118053907937514\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are non-denatured and can be used for ELISA, immunoprecipitation\/Co-IP, enzymatic activity determination and other applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eNon-Denatured Protein Solubilization Reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e10 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-010","offers":[{"title":"10 ml","offer_id":16746276192367,"sku":"Catalog # WA-010","price":84.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-010_1024x1024_4c97d74b-194f-4dec-96c1-9b5b21c653ef.png?v=1540420008"},{"product_id":"minute™-protein-solubilization-reagent-for-ms","title":"Minute™ Protein Solubilization Reagent for MS (2 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-011_v4.pdf?v=1625084192\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_95261d60-5e9d-4540-8175-1f4da3b93f82.pdf?3279434239530390899\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis reagent is formulated for dissolving protein and organelle pellets using Minute™ kits from Invent Biotechnologies or similar products of other manufacturers. Solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eProtein Solubilization Reagent for MS\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-011","offers":[{"title":"2 ml","offer_id":16746283794543,"sku":"Cat #: WA-011","price":84.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-011_1024x1024_9f445ebd-9588-4964-99e6-140127e9c099.png?v=1540420104"},{"product_id":"minute-endosome-isolation-and-cell-fractionation-kit","title":"Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/28-ED-028_v5.pdf?v=1709751079\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_de7ff84c-0ca7-4b35-8b8d-a79f38edbec4.pdf?10677628874286709938\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eEarly endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/ED-028_large.jpg?v=1540560730\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Beads\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2.5 grams\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cat #: ED-028","offers":[{"title":"20 Preps","offer_id":16746299785327,"sku":"Cat #: ED-028","price":730.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/ED-028_1024x1024_da5b4e82-26f2-40b0-815f-b1f71eee32d7.png?v=1540420183"},{"product_id":"minute-sds-remover","title":"Minute™ SDS-Remover (10 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-012_v2.pdf?v=1613418107\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_e5d20828-bab5-47c5-9d6e-aa36f5322796.pdf?221134363044597660\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eSodium dodecyl sulfate is one of the most commonly used detergents for biological research. However, the presence of SDS in the sample could interfere with downstream applications. For instance, SDS must be reduced to below a certain concentration before trypsin digestion of protein can be performed effectively. The presence of SDS in a protein sample could interfere with, and needs to be removed prior to, the analysis. The presence of SDS in protein samples also interferes with antigen-antibody binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary formulated reagent is designed for fast and easy removal of SDS in solution by precipitation. The efficacy is superior to SDS-removal column and TCA precipitation with minimum protein loss (\u0026lt;20%). Due to the small volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch5\u003eReferences (1)\u003cbr\u003e\n\u003c\/h5\u003e\n\u003cp\u003e1. Yoshimura, R., Shigeeda, W., Fujita, Y., Kokaji, T., Deguchi, H., Tomoyasu, M., ... \u0026amp; Saito, H. (2023). Diagnostic method of mass spectrometry for detecting lymph node metastasis of non‐small cell lung cancer. \u003cem\u003eThoracic Cancer\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 568.401px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 326px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 227.401px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 326px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eSDS-Remover\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 227.401px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e10 ml\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: WA-012","offers":[{"title":"10 ml","offer_id":16746309910639,"sku":"Cat #: WA-012","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-012_1024x1024_290824ef-019d-47dd-8558-dbb1a51f60cf.png?v=1540420234"},{"product_id":"minute™-nuclei-and-cytosol-isolation-kit-for-adipose-tissues","title":"Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/29-AN-029_v10.pdf?v=1650999549\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_91a88af6-f3fe-47d0-b535-4810c3254e35.pdf?9884351379084524198\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e\u003cspan style=\"color: #410f0f;\"\u003e*Invent Biotechnologies, Inc. is the inventor of the\u003cspan\u003e \u003c\/span\u003espin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the\u003cspan\u003e \u003c\/span\u003etechnology.\u003c\/span\u003e \u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eAdipocytes are the major energy storage sites in the body, and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into a single nucleus and cytosolic fraction is a common lab practice. However, when it comes to adipose tissue and adipocytes, separating these two fractions is quite challenging due to the low protein content and the presence of abundant lipid droplets. Methods developed by different labs are tedious and unpractical, requiring up to 50 grams of starting materials. This kit provides a quick and simple protocol for obtaining high-quality single nuclei and cytosol fractions from adipose tissue and adipocytes. Only milligrams of starting material are required, making it possible to isolate nuclei and extract proteins from biopsies and limited tissues. The intact nuclei isolated can be used in many applications such as scRNA-seq, ATAC-seq, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, and other applications.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFor storage and transportation of isolated single nuclei, the following buffer is recommended:\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1\u0026amp;_sid=8b67760c6\u0026amp;_ss=r\"\u003e\u003cem\u003eWA-014: Minute™ Anti-Clumping Nuclei Storage Buffer \u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWe also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute-detergent-free-nuclei-isolation-kit?_pos=1\u0026amp;_sid=844017157\u0026amp;_ss=r\"\u003eNI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps?_pos=1\u0026amp;_sid=1f449fc5a\u0026amp;_ss=r\" target=\"_blank\"\u003eBN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues\/Cells\u003c\/a\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1\u0026amp;_sid=b147abdb9\u0026amp;_ss=r\"\u003e\u003cem\u003eSN-047: Minute™ Single Nucleus Isolation Kit for Tissues\/Cells\u003c\/em\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AN-029_A_V2_large.jpg?v=1540918331\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AN-029_B_large.jpg?v=1540560824\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eN\/C Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1.5 ml Eppendorf Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestle for 1.5 ml Eppendorf Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2 ml Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (2)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eRajbhandari, P., Arneson, D., Feng, A. C., Ahn, I. S., Diamante, G., Zaghari, N., ... \u0026amp; Smale, S. T. (2019). Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes. \u003cem data-mce-fragment=\"1\"\u003eeLife 2019;8:e49501.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"mso-bidi-font-size: 12.0pt; line-height: 107%; mso-ascii-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri; mso-bidi-font-family: Calibri; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e   \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003eFelemban, A. H., Alshammari, G. M., Yagoub, A. E. A., Saleh, A., \u0026amp; Yahya, M. A. (2024). Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing Adipogenesis. \u003ci\u003eNutrients\u003c\/i\u003e, \u003ci\u003e16\u003c\/i\u003e(18), 3174.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: AN-029","offers":[{"title":"20 Preps","offer_id":16746318299247,"sku":"Cat #: AN-029","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/AN-029_1024x1024_196036db-25b2-4cf8-99d4-9aab7e67e2f0.png?v=1569162674"},{"product_id":"minute™-hi-efficiency-saliva-exosome-isolation-kit","title":"Minute™ High-Efficiency Saliva Exosome Isolation Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/30-SE-030_v3.pdf?v=1613417810\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_d63e953e-c038-4a84-9a76-a03aa00fd612.pdf?16908346773917556967\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDiagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris, large amount of amylase, mucin and glycoprotein are present in saliva, and make the samples viscous and hard to manipulate. Pre-treatments, such as sonication and dilution, are often required. In many cases, the saliva samples can still be difficult to handle even after pre-treatments. This product is specifically designed to address these issues using the proprietary saliva filters. Highly viscous saliva samples can be converted to non-viscous solutions instantly by passing the samples through the filter. Exosomes can then be readily precipitated from as little as 100 µl saliva using the highly effective non-PEG-based reagent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SE-030_V2_large.jpg?v=1540918411\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eExosome Precipitation Reagent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0 ml Collection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (2)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eHyun, K. A., Gwak, H., Lee, J., Kwak, B., \u0026amp; Jung, H. I. Salivary Exosome and Cell-Free DNA for Cancer Detection.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eJia, Y., Wang, C., Sun, H., \u0026amp; Ni, Z. (2019). Microfluidic Approaches Toward the Isolation and Detection of Exosome Nanovesicles. \u003ci\u003eIEEE Access\u003c\/i\u003e.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: SE-030","offers":[{"title":"50 Preps","offer_id":16746330521711,"sku":"Cat #: SE-030","price":394.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SE-030_1024x1024_e8606ec8-9022-4884-bd5c-59a80d15ba1d.png?v=1540420550"},{"product_id":"minute™-cell-suspension-isolation-kit-from-fresh-frozen-tissues","title":"Minute™ Cell Suspension Isolation Kit for Fresh\/Frozen Tissues (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/31-CS-031_v2_d66eb9c1-7759-441d-8f4b-9d9cbba5f089.pdf?v=1741877835\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-5_c6bd4428-c11d-4ad3-b125-9d1ad61c5910.pdf?789689941342858370\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"font-family: 'Times New Roman',serif;\"\u003eObtaining quality cell suspension from fresh and\/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification. Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time consuming. Most importantly, cells in the tissue especially frozen tissue are usually damaged and show low integrity and viability. We have developed a simple, rapid, efficient, and instrument-free method for isolating cell suspension from fresh or frozen tissues using a combination of chemical cell protection and physical separation mechanisms. The protocol can be finished in about 20 min. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/CS-031_large.jpg?v=1540563251\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTissue Dissociation Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1.5 ml Microfuge Tube\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePestles for 1.5 ml Tube\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (3)\u003c\/h5\u003e\n\u003cp\u003e1. Zhang, X., Zhang, X., Li, X., Ma, J., Xie, D., \u0026amp; Jiang, N. (2020). Dishevelled-1 regulated apoptosis through NF-κB in Cerebral Ischemia\/Reperfusion Injury in Rats. \u003ci\u003eNeuroscience Letters\u003c\/i\u003e, 134862.\u003c\/p\u003e\n\u003cp\u003e2. Xu, H., Liu, L., Cong, M., Liu, T., Sun, S., Ma, H., ... \u0026amp; Wang, P. (2020). EGF neutralization antibodies attenuate liver fibrosis by inhibiting myofibroblast proliferation in bile duct ligation mice. \u003ci\u003eHistochemistry and Cell Biology\u003c\/i\u003e, 1-10.\u003c\/p\u003e\n\u003cp\u003e3.  Hamamura, K., Yoshida, Y., Oyama, K., Li, J., Kawano, S., Inoue, K., ... \u0026amp; Aritake, K. (2024). Hematopoietic Prostaglandin D Synthase Is Increased in Mast Cells and Pericytes in Autopsy Myocardial Specimens from Patients with Duchenne Muscular Dystrophy. \u003cem\u003eInternational Journal of Molecular Sciences\u003c\/em\u003e, \u003cem\u003e25\u003c\/em\u003e(3), 1846.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: CS-031","offers":[{"title":"50 Preps","offer_id":16746371088495,"sku":"Cat #: CS-031","price":378.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/CS-031_1024x1024_7ca62b44-3bf9-4164-b10c-b1aeb58a192b.png?v=1540421090"},{"product_id":"minute-protein-nucleic-acid-extraction-kit-from-gels","title":"Minute™ Protein\/Nucleic Acid Extraction Kit from Gels (20 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/19-PN-019_v3.pdf?v=1613417743\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_df711bfd-8177-41cb-b65d-8c248c2c483e.pdf?18287592235982684526\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePassive elution and electroelution are the most common methods for extracting proteins\/nucleic acid from polyacrylamide\/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electroelution is faster (30 minutes to 2 hours) than passive elution, but it requires a special elution device that works less effectively for larger proteins (\u0026gt;70 kDa). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein\/nucleic acid extraction kit. Proteins\/nucleic acid can be extracted from gels in less than 10 minutes with a high yield. The elution buffer can be a detergent-containing buffer or pure water depending on the method of gel staining. The elution volume is between 10 and 200 µl. Multiple gel pieces can be processed in a single tube, and the final protein concentration is relatively high.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/PN-019_V2_large.jpg?v=1540918377\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable height=\"129\" width=\"174\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 26px;\"\u003e\n\u003ctd style=\"height: 26px; width: 352px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 26px; width: 178px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: left; height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 352px;\"\u003e\n\u003cdiv\u003e\u003cspan style=\"font-weight: 400;\"\u003eFilter Cartridges\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 178px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 units\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: left; height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 352px;\"\u003e\n\u003cdiv\u003e\u003cspan style=\"font-weight: 400;\"\u003eMicrofuge Tubes\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 178px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e20 units\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: left; height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 352px;\"\u003e\n\u003cdiv\u003e\u003cspan style=\"font-weight: 400;\"\u003eExtraction Powder\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 178px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 grams\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: left; height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 352px;\"\u003e\n\u003cdiv\u003e\u003cspan style=\"font-weight: 400;\"\u003eMicro-Pestles\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 178px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: PN-019","offers":[{"title":"20 preps","offer_id":16746382753903,"sku":"Cat #: PN-019","price":268.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/PN-019_1024x1024_3f0e96b3-7fd5-4c77-9a48-4c079025f31f.png?v=1540421074"},{"product_id":"minute-histone-dna-binding-protein-extraction-kit","title":"Minute™ Histone\/DNA Binding Protein Extraction Kit (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/14-HP-014_v2.pdf?v=1613417712\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_62d9d0e5-9807-48a2-b816-1c5bf769659e.pdf?14518220220387285896\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eHistones and other DNA-binding proteins play an important role in chromosome organization, gene regulation and post-translational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. Minute™ Histone\/DNA Binding Protein Extraction Kit is designed to efficiently extract histones and other DNA-binding proteins from cultured cells and isolated cells. Unlike many other commercial histone extraction kits that employ acid or high-salt extraction methods, Minute™ Histone\/DNA Binding Protein Extraction Kit is efficient in extracting histones and other DNA-binding proteins that can be used for internal controls for histone modification analysis. In comparison, acid extraction protocol that selectively extracts basic proteins and a good internal control is usually not possible. Minute™ Histone\/DNA Binding Protein Extraction Kit\u003c\/span\u003e \u003cspan style=\"font-weight: 400;\"\u003ecan extract histones from 0.5 to 5 million cells in less than 10 minutes with high protein yield (1-2.5 mg\/ml), making it the fastest and most robust histone extraction kit available on the market.\u003c\/span\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/HP-014_large.jpg?v=1540563193\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes with Caps\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003cp\u003e1. Zhao, X., Xue, X., Wang, J., Dai, S., Peng, C., \u0026amp; Li, Y. (2022). Quercetin alleviates ethanol‐induced hepatic steatosis in L02 cells by activating TFEB translocation to compensate for inadequate autophagy. \u003cem\u003ePhytotherapy Research\u003c\/em\u003e.\u003c\/p\u003e","brand":"Cat #: HP-014","offers":[{"title":"50 Preps","offer_id":16746395631727,"sku":"Cat #: HP-014","price":473.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/HP-014_1024x1024_09b80962-671c-4bc8-8ab3-77982d67c01d.png?v=1540421183"},{"product_id":"minute-single-cell-isolation-kit-for-fresh-and-fixed-tissues","title":"Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/12-SC-012_V7.pdf?v=1646765273\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_e06e3509-b27e-4472-91aa-bd1e68f660ec.pdf?14503251849663878283\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMinute\u003csup\u003eTM\u003c\/sup\u003e single cell isolation kit comprises optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to isolate single cells from fresh animal tissues rapidly, and it can also isolate nuclei from tissues briefly fixed with formaldehyde for chromosome immunoprecipitation (ChIP). The tissue disaggregation buffers are formulated to disaggregate animal tissues gently. The buffers don’t contain any proteinases and EDTA that may adversely affect cell surface markers. Due to filter cartridges with pre-defined pore size and a specially formulated buffer system, single-cell suspension can be obtained from fresh tissues in less than 10 min. This kit is specifically designed for lymphoid tissues such as the spleen, thymus, and lymph node. For other tissues, the performance is tissue type-dependent. For cell suspension isolation from frozen\/fresh tissues, please refer to Cat# CS-031.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SC-012_A_large.jpg?v=1540563096\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SC-012_B_large.jpg?v=1540563120\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit includes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer A (for non-fixed tissues)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eBuffer B (for fixed tissues)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e25 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eCell Isolation Filter Cartridges\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eCollection Tubes with Caps\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e50 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePlastic Rods\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (8)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eKuo C-T, et al. (2016). Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots. Nature Communications. http:\/\/www.nature.com\/naturecommunications.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBultmann-Mellin, I., Dinger, K., Debuschewitz, C., Loewe, K. M., Melcher, Y., Plum, M. T., ... \u0026amp; Jüngst, C. (2017). Role of LTBP-4 in alveolarization, angiogenesis and fibrosis in lungs. American Journal of Physiology-Lung Cellular and Molecular Physiology, ajplung-00031.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eVergara, M. N., Flores-Bellver, M., Aparicio-Domingo, S., McNally, M., Wahlin, K. J., Saxena, M. T., ... \u0026amp; Canto-Soler, M. V. (2017). Enabling quantitative screening in retinal organoids: 3D automated reporter quantification technology (3D-ARQ). Development, dev-146290.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eJanicova, A., Becker, N., Xu, B., Wutzler, S., Vollrath, T., Hildebrand, F., ... \u0026amp; Relja, B. (2019). Endogenous uteroglobin as intrinsic anti-inflammatory signal modulates monocyte and macrophage subsets distribution upon sepsis induced lung injury. \u003cem\u003eFrontiers in Immunology\u003c\/em\u003e, \u003cem\u003e10\u003c\/em\u003e, 2276.\u003c\/li\u003e\n\u003cli\u003eTanaka, M., Saka-Tanaka, M., Ochi, K., Fujieda, K., Sugiura, Y., Miyamoto, T., ... \u0026amp; Aoe, S. (2020). C-type lectin Mincle mediates cell death–triggered inflammation in acute kidney injury. \u003cem\u003eJournal of Experimental Medicine\u003c\/em\u003e, \u003cem\u003e217\u003c\/em\u003e(11).\u003c\/li\u003e\n\u003cli\u003eBecker, N., Störmann, P., Janicova, A., Köhler, K., Horst, K., Dunay, I. R., ... \u0026amp; Relja, B. (2021). Club Cell Protein 16 Attenuates CD16brightCD62dim Immunosuppressive Neutrophils in Damaged Tissue upon Posttraumatic Sepsis-Induced Lung Injury. \u003cem\u003eJournal of Immunology Research\u003c\/em\u003e, \u003cem\u003e2021\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003eGünther, H. S., Henne, S., Oehlmann, J., Urban, J., Pleizier, D., Renevier, N., ... \u0026amp; Wülfing, C. (2021). GFAP and desmin expression in lymphatic tissues leads to difficulties in distinguishing between glial and stromal cells. \u003cem\u003eScientific Reports\u003c\/em\u003e, \u003cem\u003e11\u003c\/em\u003e(1), 1-14.\u003c\/li\u003e\n\u003cli\u003eKawada, M., Yokoi, H., Kimura, T., Matsumoto, Y., Sakurai, H., Matsumoto, K., ... \u0026amp; Saito, K. (2021). Involvement of galanin and galanin receptor 2 in a mouse model of allergic rhinitis. \u003cem\u003eAllergology International\u003c\/em\u003e.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: SC-012","offers":[{"title":"50 Preps","offer_id":16746398875759,"sku":"Cat #: SC-012","price":384.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SC-012_1024x1024_c443db8c-2ae4-4427-b12e-991b441334a2.png?v=1569162678"},{"product_id":"minutetm-albumin-depletion-reagent-for-plasma-and-serum","title":"Minute™ Albumin Depletion Reagent for Plasma and Serum (20 ml)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-013_v2.pdf?v=1613418115\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?6101520673852382130\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHuman\/animal serum and plasma are the best sources of samples for biomarker identification. However, the presence of albumin in large amounts interferes with many assays and functional proteomic analysis. Serum albumin accounts for 50-70% of the total serum protein that causes loss of resolution for 1D and 2D electrophoresis. Effective removal of albumin using our reagent can increase sensitivity of the assay for many folds. Unlike a resin-based depletion kit (that only deals with microliter amounts of a sample), Minute albumin depletion reagent can handle a few microliters and up to several milliliters of samples. Over 90% of albumin can be removed in less than 5 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/AlbuminDepletion_large.jpg?v=1541177970\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 395.75px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 136.25px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 395.75px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAlbumin Depletion Reagent \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 136.25px;\"\u003e\n\u003cp\u003e\u003cspan\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\n\u003cstrong\u003eReferences (4):\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h5\u003e\n\u003cp\u003e1.  Kumadaki, K., Suzuki, N., Tatematsu, K., Doi, Y., \u0026amp; Tsukamoto, K. (2023). Comparison of Biological Activities of BafA Family Autotransporters within Bartonella Species Derived from Cats and Rodents. \u003cem\u003eInfection and Immunity\u003c\/em\u003e, e00186-22.\u003c\/p\u003e\n\u003cp\u003e2.  Kim, K. H. (2023). Intranasal delivery of mitochondrial protein humanin rescues cell death and promotes mitochondrial function in Parkinson's disease. \u003cem\u003eTheranostics\u003c\/em\u003e, \u003cem\u003e13\u003c\/em\u003e(10), 3330.\u003c\/p\u003e\n\u003cp\u003e3.  Debande, L., Sabbah, A., Kuhn, L., Ngondo, P., Andre, A. C., Roche, B., ... \u0026amp; Marteyn, B. S. (2023). SPATEs promote the survival of Shigella to the plasma complement system upon hemorrhage and bacteremia. \u003cem\u003ebioRxiv\u003c\/em\u003e, 2023-11.\u003c\/p\u003e\n\u003cp\u003e4.  \u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-font-kerning: 0pt; mso-ligatures: none;\"\u003eKim, C. Y., Kim, J., Yoon, S., Yi, I. J., Lee, H., Seo, S., ... \u0026amp; Yi, S. S. (2024). Advancing the early detection of canine cognitive dysfunction syndrome with machine learning-enhanced blood-based biomarkers. \u003ci\u003eFrontiers in Veterinary Science\u003c\/i\u003e, \u003ci\u003e11\u003c\/i\u003e, 1390296.\u003c\/span\u003e\u003cspan style=\"font-size: 14.0pt; mso-bidi-font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman';\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Cat #: WA-013","offers":[{"title":"20 ml","offer_id":16800253411439,"sku":"Cat #: WA-013","price":189.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/WA-013_1024x1024_9b5ea9b5-dbd0-48f8-8369-751757742493.png?v=1569164352"},{"product_id":"minute™-er-enrichment-kit","title":"Minute™ ER Enrichment Kit (20 Preps)","description":"\u003ch2 class=\"p1\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/36-ER-036_v6.pdf?v=1738352242\"\u003e\u003cb\u003eManual \u0026amp; Protocol\u003c\/b\u003e\u003c\/a\u003e\u003cspan class=\"s1\"\u003e\u003cb\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?6101520673852382130\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe endoplasmic reticulum (ER) is a significant membranous structure that bridges the nuclear membrane and the plasma membrane, serving as a pivotal player in the exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in the cytoplasm are directed to the ER, from where vesicles transport protein cargo to the Golgi apparatus, leading to subsequent fusion with the plasma membrane.\u003c\/p\u003e\n\u003cp\u003eTraditional ER isolation methods rely on density gradient ultracentrifugation, a process demanding a substantial amount of starting material. These methods can be laborious, time-consuming, and often result in notable cross-contamination. Remarkably, all existing commercial kits for ER isolation are based on techniques developed in the 1970s. In a departure from these traditional approaches, the Minute™ ER enrichment kit stands out in the market by harnessing patented spin-column-based technology. This innovative method is not only straightforward and rapid but also requires only a small quantity of starting cultured cells or tissues.\u003c\/p\u003e\n\u003cp\u003eThis kit excels at differentially precipitating native ER, primarily rough ER, from cultured cells and tissues, all without the need for a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in approximately two hours.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/comparison-of-major-er-isolation-kits-1\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003eCompare with Other ER Isolation Kits\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/ER-G_Product_Fig._036_large.jpg?v=1550679654\"\u003e\u003c\/p\u003e\n\u003ctable height=\"129\" style=\"width: 526px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 36px; width: 197.25px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 36px; width: 311.75px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 4px; width: 197.25px;\"\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 4px; width: 311.75px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 12px; width: 197.25px;\"\u003eBuffer B\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 12px; width: 311.75px;\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 22px; width: 197.25px;\"\u003eBuffer C\u003c\/td\u003e\n\u003ctd style=\"height: 22px; text-align: center; width: 311.75px;\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 22px; width: 197.25px;\"\u003eBuffer D\u003c\/td\u003e\n\u003ctd style=\"height: 22px; text-align: center; width: 311.75px;\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 22px; width: 197.25px;\"\u003ePlastic Rods\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 311.75px; text-align: center;\"\u003e2 Units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 22px; width: 197.25px;\"\u003eFilter Cartridge\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 311.75px; text-align: center;\"\u003e20 Units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"height: 22px; width: 197.25px;\"\u003eCollection Tubes\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 311.75px; text-align: center;\"\u003e20 Units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e","brand":"Cat #: ER-036","offers":[{"title":"20 Preps","offer_id":17152298745967,"sku":"Cat #: ER-036","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/ER-036_1024x1024_d188ca5f-7f05-4648-bb2d-5920a20fc007.png?v=1550679616"},{"product_id":"minutetm-high-fidelity-er-isolation-kit-for-liver-tissue","title":"Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)","description":"\u003ch2 class=\"p1\"\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/35-ER-035_v4.pdf?v=1706118925\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?6101520673852382130\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eEndoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation. The whole protocol can be done in about 2 hours.\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/ER-035_24fac874-f3b1-477e-a6ad-60c1f9f99b50_large.jpg?v=1541107674\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Pages_from_33-TableRunner_Single_large.jpg?v=1550680006\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e4 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e4 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridge\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: ER-035","offers":[{"title":"20 Preps","offer_id":17153293353071,"sku":"Cat #: ER-035","price":730.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/ER-035_1024x1024_998b3fc3-c8d1-4b92-a0c0-509ddfc6599e.png?v=1550680020"},{"product_id":"minute™-lysosome-isolation-kit-for-mammalian-cells-tissues","title":"Minute™ Lysosome Isolation Kit for Mammalian Cells\/Tissues (20 Preps)","description":"\u003ch2 class=\"p1\"\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/34-LY-034_v5.pdf?v=1709826888\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?325\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e \u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eLysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes crucial for breaking down excess or worn-out organelles, food particles, as well as viruses and bacteria that have been engulfed. Lysosomes exhibit a relatively large size, ranging from 0.1 to 1.2 micrometers. In the realm of cell research, the initial isolation of lysosomes is a pivotal step when investigating processes like autophagy, protein degradation, and recycling.\u003c\/p\u003e\n\u003cp\u003eTraditional methods for lysosome isolation, which originated in the 1970s, are associated with challenges such as the need for substantial starting material and a time-consuming procedure that often leads to significant cross-contaminations. However, a groundbreaking solution is offered by this kit, utilizing patented spin-column-based technology that is characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be notably enriched without the requirement of a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003ca href=\"https:\/\/inventbiotech.com\/blogs\/helpful-blog\/comparison-of-major-lysosome-isolation-kits-1\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003eCompare with Other Lysosome Isolation Kits\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_A_1bdfa113-6b92-4a86-b09a-517f73e42ab7_large.jpg?v=1541108023\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_bargraph_A_large.png?v=1541108107\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_bargraph_B_large.png?v=1541108120\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/LY-034_B_large.jpg?v=1541108040\"\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 544.891px;\" height=\"204\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px; text-align: center;\"\u003e2 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 404px;\"\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 123.891px;\"\u003e\n\u003cp style=\"text-align: center;\"\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: LY-034","offers":[{"title":"20 Preps","offer_id":17154144338031,"sku":"Cat #: LY-034","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/LY-034_1024x1024_d1be844a-d953-423e-b655-4434c36b7943.png?v=1541099748"},{"product_id":"minute™-detergent-free-nuclear-matrix-protein-extraction-kit-for-mammalian-cultured-cells-tissues-20-preps","title":"Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells\/Tissues) (20 Preps)","description":"\u003ch2\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/33-NM-033_v3.pdf?v=1613417831\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6.pdf?6101520673852382130\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eNucleoplasm, also referred to as karyoplasm\/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: \u003cstrong\u003ecytosolic, nuclear matrix and water-insoluble fractions\u003c\/strong\u003e (mainly envelope and nucleic acid-associated proteins) using a patented spin-column-based technology. The buffers used are detergent and EDTA-free. The whole protocol can be completed in less than 1 hour. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis and protein trafficking studies.  \u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NM-033_Final_data_480x480.jpg?v=1589474642\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NM-033_B_large.jpg?v=1541108569\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/NM-033_C_large.jpg?v=1541108586\"\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eBuffer B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Filter Cartridges\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e20 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003col\u003e\n\u003cli\u003eLuo, Q., Wu, X., Zhao, P., Nan, Y., Chang, W., Zhu, X., ... \u0026amp; Liu, Z. (2021). OTUD1 Activates Caspase‐Independent and Caspase‐Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. \u003cem\u003eAdvanced Science\u003c\/em\u003e, 2002874.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Cat #: NM-033","offers":[{"title":"20 Preps","offer_id":17154482897007,"sku":"Cat #: NM-033","price":629.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/NM-033_1024x1024_598eb002-92ff-469a-a68f-4910134a554b.png?v=1589474740"},{"product_id":"minute™-total-protein-extraction-kit-for-skin-tissue","title":"Minute™ Total Protein Extraction Kit for Skin Tissue (50 Preps)","description":"\u003ch2 class=\"p1\"\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-01-SK_v2.pdf?v=1613417962\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-7_a9161227-8f44-49cc-86c3-15f9f52aece9.pdf?v=1706714461\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eSkin tissue is made up of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal skin tissues by using a combination of mechanical extraction and chemical lysis of skin tissues. The kit features a simple and fast single-tube protocol and optimized buffers for skin tissues. The researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for skin tissue. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-5 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping and storage: \u003c\/strong\u003eThis kit is shipped and stored at room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-01_grande.jpg?v=1541178029\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges  \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SA-01-SK","offers":[{"title":"50 Preps","offer_id":17155113058415,"sku":"Cat #: SA-01-SK","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SA-01-SK_1024x1024_2fb41b3f-8a34-4805-867f-390b4897d813.png?v=1541101102"},{"product_id":"minute™-total-protein-extraction-kit-for-bone-tissue","title":"Minute™ Total Protein Extraction Kit for Bone Tissue (50 Preps)","description":"\u003ch2 class=\"p1\"\u003e\u003cb\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-02-BT_v2.pdf?v=1613417970\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-7_a9161227-8f44-49cc-86c3-15f9f52aece9.pdf?v=1706714461\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eBone tissues are commonly used for research. There are two types of bone tissues: compact and spongy. Cells in bone tissues are tightly packed and, because of their unique structures, it is very difficult to efficiently extract total protein from them. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for extracting proteins from human or animal bone tissue by a combination of mechanical extraction and chemical lysis of cells in tissues. The kit features a simple and fast single-tube protocol and optimized buffers for bone tissue. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for bone tissue. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 0.5-2 mg\/ml depending upon type of bones. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping and storage: \u003c\/strong\u003eThis kit is shipped and stored at room temperature.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-02_grande.jpg?v=1541178115\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges  \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cstrong\u003eReferences (10):\u003c\/strong\u003e\u003c\/h5\u003e\n\u003cp\u003e1. \u003cspan style=\"color: #000000;\"\u003eRen, H., Wang, Z., Xu, J., Chen, J., \u0026amp; Lan, J. (2020). The Impact of Frizzled-9 on Dental Implant Osseointegration in Hyperlipidemic Rats. \u003cem\u003eJournal of Hard Tissue Biology\u003c\/em\u003e, \u003cem\u003e29\u003c\/em\u003e(1), 37-44.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e2. \u003c\/span\u003ePedersen, K. B., Osborn, M. L., Robertson, A. C., Williams, A. E., Watt, J., Denys, A., ... \u0026amp; Ronis, M. J. (2020). Chronic Ethanol Feeding in Mice Decreases Expression of Genes for Major Structural Bone Proteins in a Nox4-Independent Manner. \u003cem\u003eJournal of Pharmacology and Experimental Therapeutics\u003c\/em\u003e, \u003cem\u003e373\u003c\/em\u003e(3), 337-346.\u003c\/p\u003e\n\u003cp\u003e3.  \u003cspan data-mce-fragment=\"1\"\u003eLiu, H. D., Ren, M. X., Li, Y., Zhang, R. T., Ma, N. F., Li, T. L., ... \u0026amp; Yang, M. (2022). Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells and promotes osteogenesis by activating SIRT1. \u003c\/span\u003e\u003ci data-mce-fragment=\"1\"\u003eFree Radical Research\u003c\/i\u003e\u003cspan data-mce-fragment=\"1\"\u003e, (just-accepted),\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e 1-19.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e4.  \u003c\/span\u003eJiang, C., Lin, Y., Shan, H., Xia, W., Pan, C., Wang, N., ... \u0026amp; Yu, X. (2022). miR-146a Protects against Staphylococcus aureus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis. \u003cem\u003eACS Infectious Diseases\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e5. Song, D., Ha, C. Z., Xu, Q., \u0026amp; Hu, Y. H. (2022). mRNA expression profiling of the cancellous bone in patients with idiopathic osteonecrosis of the femoral head by whole-transcriptome sequencing. \u003cem\u003eMedicine\u003c\/em\u003e, \u003cem\u003e101\u003c\/em\u003e(35), e30213.\u003c\/p\u003e\n\u003cp\u003e6. Deng, J., Cohen, D. J., Sabalewski, E. L., Van Duyn, C., Wilson, D. S., Schwartz, Z., \u0026amp; Boyan, B. D. (2022). Semaphorin 3A Delivered by a Rapidly Polymerizing Click Hydrogel Overcomes Impaired Implant Osseointegration in a Rat Type 2 Diabetes Model. \u003cem\u003eActa Biomaterialia\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e7.  He, H., Zhang, Y., Sun, Y., Zhang, Y., Xu, J., Yang, Y., \u0026amp; Chen, J. (2022). Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway. \u003ci\u003eFrontiers in cell and developmental biology\u003c\/i\u003e, \u003ci\u003e9\u003c\/i\u003e, 3661.\u003c\/p\u003e\n\u003cp\u003e8.  Choi, R. Y., Kim, I. W., Ji, M., Paik, M. J., Ban, E. J., Lee, J. H., ... \u0026amp; Seo, M. (2023). Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice. \u003ci\u003eBiomedicine \u0026amp; Pharmacotherapy\u003c\/i\u003e, \u003ci\u003e165\u003c\/i\u003e, 115112.\u003c\/p\u003e\n\u003cp\u003e9.  Liu, Z., Yao, X., Jiang, W., Zhou, Z., \u0026amp; Yang, M. (2023). Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα\/NOX4\/ROS\/NF-κB pathways. \u003ci\u003eJournal of Orthopaedic Surgery and Research\u003c\/i\u003e, \u003ci\u003e18\u003c\/i\u003e(1), 1-14.\u003c\/p\u003e\n\u003cp\u003e10.  Zhang, C. X., \u0026amp; Man, Q. W. (2024). Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling. \u003ci\u003eBritish Journal of Oral and Maxillofacial Surgery\u003c\/i\u003e.\u003c\/p\u003e","brand":"Cat #: SA-02-BT","offers":[{"title":"50 Preps","offer_id":17155455680623,"sku":"Cat #: SA-02-BT","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SA-02-BT_1024x1024_be409286-1c33-414b-b188-5791d66cd1c8.png?v=1541101436"},{"product_id":"minute™-total-protein-extraction-kit-for-blood-vessels","title":"Minute™ Total Protein Extraction Kit for Blood Vessels (50 Preps)","description":"\u003ch2 class=\"p1\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-03-BV_v2.pdf?v=1613417978\"\u003e\u003cb\u003eManual \u0026amp; Protocol\u003c\/b\u003e\u003c\/a\u003e\u003cspan class=\"s1\"\u003e\u003cb\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-7_a9161227-8f44-49cc-86c3-15f9f52aece9.pdf?v=1706714461\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp class=\"p1\"\u003e\u003cspan class=\"s1\"\u003eBlood vessels (arteries and veins) are made of specialized connective tissues. There are three layers of tissues in blood vessels: tunica, tunica media and endothelium. Due to their unique structures, blood vessels are notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased with solution-based (incomplete protein profile) methods. This kit provides a highly efficient method for total protein extraction from human or animal blood vessels by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than the RIPA buffer. The kit features a simple, fast single-tube protocol and optimized buffers for blood vessels. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for blood vessels. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping and storage: \u003c\/strong\u003eThis kit is shipped and stored at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-03_grande.jpg?v=1541178181\"\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges  \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cbr\u003e\n\u003c\/div\u003e\nCollection Tubes\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003e References (3)\u003cbr\u003e\n\u003c\/h5\u003e\n\u003cp\u003e1. Chen, D., Li, H., Wang, X., Zhang, L., Ji, Z., \u0026amp; Zhang, J. (2022). Hypertriglyceridemia impairs urethral spontaneous tone through down-regulation of ANO1 in mouse urethral smooth muscle cells. \u003cem\u003eUrology\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e2.  Xia, M. (2023). \u003cem\u003eSenescence signaling pathway in the process of vascular calcification both in vitro and ex vivo\u003c\/em\u003e (Doctoral dissertation).\u003c\/p\u003e\n\u003cp\u003e3.  \u003cspan data-mce-fragment=\"1\"\u003eDu, F., Zhu, Z., Lai, Z., Li, K., Chen, J., Zhang, E., ... \u0026amp; Liu, B. (2023). Imbalance of helper T cell subtypes and adipokine secretion in perivascular adipose tissue as a trigger of atherosclerosis in chronic Porphyromonas gingivalis W83 infection. \u003c\/span\u003e\u003ci data-mce-fragment=\"1\"\u003eMicrobes and Infection\u003c\/i\u003e\u003cspan data-mce-fragment=\"1\"\u003e, 105181.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cat #: SA-03-BV","offers":[{"title":"50 Preps","offer_id":17156035084399,"sku":"Cat #: SA-03-BV","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SA-03-BV_1024x1024_02e560a6-c96b-416b-aa34-3ab61810fa67.png?v=1541102267"},{"product_id":"minute™-total-protein-extraction-kit-for-tendons","title":"Minute™ Total Protein Extraction Kit for Tendons (50 Preps)","description":"\u003ch2 class=\"p1\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-04-TD_v2.pdf?v=1613417985\"\u003e\u003cb\u003eManual \u0026amp; Protocol\u003c\/b\u003e\u003c\/a\u003e\u003cspan class=\"s1\"\u003e\u003cb\u003e\u003cspan style=\"color: rgb(246, 146, 30);\"\u003e |\u003c\/span\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-7_a9161227-8f44-49cc-86c3-15f9f52aece9.pdf?v=1706714461\"\u003eMSDS\u003c\/a\u003e\u003c\/b\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eA tendon\/ligament is a fibrous connective tissue that attaches muscles to bone. Histologically tendons consist of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also very difficult to lyse the tendon tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased (incomplete protein profile) with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal tendons by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for tendons. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for tendons. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping and storage: \u003c\/strong\u003eThis kit is shipped and stored at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-04_grande.jpg?v=1541178228\"\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eItems\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDenaturing Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNative Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e25 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProtein Extraction Powder\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ePlastic Rods\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e2 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFilter Cartridges  \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eCollection Tubes\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cp\u003e50 units\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Cat #: SA-04-TD","offers":[{"title":"50 Preps","offer_id":17156242145391,"sku":"Cat #: SA-04-TD","price":457.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SA-04-TD_1024x1024_2af8929c-b9cf-4391-80b3-4ede9515a22b.png?v=1541102472"}],"url":"https:\/\/inventbiotech.com\/collections\/all-products.oembed?page=3","provider":"Invent Biotechnologies Inc.","version":"1.0","type":"link"}